FastQCFastQC Report
Thu 2 Feb 2017
SRR4062662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405699
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31300.2226650228818545No Hit
GTACATGGAAGCAGTGGTATCAACG22260.15835538049041792No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21980.15636348891192212No Hit
TATCAACGCAGAGTACTTTTTTTTT20860.14839592259793882No Hit
TCCATGTACTCTGCGTTGATACCAC19330.13751165790115807No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15110.10749100625382817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1350.015.4723682
GCGTTAT1300.015.3360281
GTCTTAA551.9709437E-413.8090631
AAGGCGT655.447241E-513.1550066
TCCCCGA1750.013.0297213
TGTGCGA604.087315E-412.66823510
GTCCTAA3450.012.3831271
CGCGTAT853.9386578E-612.2952017
ACGCGTA853.9386578E-612.2952016
GTTTAGA853.971745E-612.2860041
TAACCTG1103.8131475E-812.0906835
ATCCCCG1900.012.00148512
CGTCTTA1459.640644E-1111.79498415
TGGCGAG6050.011.77873818
GCATCGC2100.011.76294114
TCTAGGG1052.7296846E-711.7583313
CGCGGTC1302.6084308E-911.69375610
CGCATCG2050.011.58638713
ATCGTAA1406.87578E-1011.5281021
CGTCGTA2000.011.40141110
Produced by FastQC (version 0.11.5)