##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062662_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1405699
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.121595732799125	32.0	32.0	32.0	32.0	32.0
2	30.733743852702464	32.0	32.0	32.0	32.0	32.0
3	30.71612272613127	32.0	32.0	32.0	32.0	32.0
4	30.80276858701614	32.0	32.0	32.0	32.0	32.0
5	30.57216658758383	32.0	32.0	32.0	32.0	32.0
6	34.22050239773949	36.0	36.0	36.0	32.0	36.0
7	34.10196564129305	36.0	36.0	36.0	32.0	36.0
8	34.07921610529708	36.0	36.0	36.0	32.0	36.0
9	34.27964379287458	36.0	36.0	36.0	32.0	36.0
10	33.89562986101576	36.0	36.0	36.0	32.0	36.0
11	34.26300722985504	36.0	36.0	36.0	32.0	36.0
12	34.018854676570164	36.0	36.0	36.0	32.0	36.0
13	34.14545076862116	36.0	36.0	36.0	32.0	36.0
14	34.0419172240999	36.0	36.0	36.0	32.0	36.0
15	33.97759264252162	36.0	36.0	36.0	32.0	36.0
16	33.983399006472936	36.0	36.0	36.0	32.0	36.0
17	33.90489855936442	36.0	36.0	36.0	32.0	36.0
18	33.92928856035325	36.0	36.0	36.0	32.0	36.0
19	33.90464174762876	36.0	36.0	36.0	32.0	36.0
20	33.89143337229378	36.0	36.0	36.0	32.0	36.0
21	33.8682363720825	36.0	36.0	36.0	32.0	36.0
22	33.81913268772333	36.0	36.0	36.0	32.0	36.0
23	33.7910349228391	36.0	36.0	36.0	32.0	36.0
24	33.75979921732889	36.0	36.0	36.0	32.0	36.0
25	33.30548289498677	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	75.0
5	126.0
6	255.0
7	51.0
8	193.0
9	274.0
10	157.0
11	67.0
12	87.0
13	81.0
14	308.0
15	365.0
16	523.0
17	694.0
18	933.0
19	1342.0
20	2144.0
21	3144.0
22	4559.0
23	6855.0
24	9396.0
25	13095.0
26	18208.0
27	23260.0
28	31338.0
29	41304.0
30	53483.0
31	72573.0
32	103020.0
33	148573.0
34	321129.0
35	548074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.429197923662734	17.456557992873975	10.885545323118603	26.228698760344688
2	16.609468684816356	20.183844558081564	37.21504164639126	25.99164511071082
3	19.351529401720953	24.03133148123775	28.322762634149466	28.29437648289183
4	12.697663928098535	15.33334898635981	35.66599002902213	36.30299705651952
5	14.54308019670218	36.429257669890475	33.65357928222208	15.374082851185268
6	34.12947518408912	35.086396977707174	16.99064643643605	13.793481401767654
7	29.78405025894748	30.569186139635875	21.00247379380306	18.64428980761359
8	27.916541170824583	33.21499342437389	19.72844479856226	19.14002060623926
9	27.341832364060252	14.301044564724346	18.787282358612764	39.56984071260264
10	16.118748357384664	26.672440600536607	31.762785334302933	25.446025707775792
11	37.24015370863027	21.03835236884373	22.625796410853624	19.095697511672384
12	24.708740941674105	23.676556593122047	28.99013334340111	22.62456912180274
13	29.60090942308473	19.584648357119505	25.339484475062445	25.474957744733317
14	23.255860334916985	20.14865413517768	25.0630017166098	31.532483813295535
15	24.91230819086011	27.708190147892015	22.488560002563986	24.890941658683893
16	25.407535444135114	25.66406786927814	24.494715873602583	24.43368081298416
17	23.612403078689493	26.04756213434152	25.707527347372526	24.63250743959646
18	24.47977251244251	24.669749126869757	27.15212446851781	23.69835389216993
19	25.387383062066053	25.210368965760416	25.240643006376068	24.161604965797455
20	25.24745528995556	24.674089791173877	25.58762841072343	24.49082650814713
21	26.19177364011759	24.35354698818181	24.472861474812536	24.981817896888064
22	25.37498040668595	25.127890904427375	24.92882283369195	24.568305855194723
23	24.101911263625293	24.726247407801065	25.74560225570284	25.4262390728708
24	24.64683032397365	25.43434295943758	25.481287403288388	24.43753931330038
25	24.62454312260164	24.934197544847176	25.63563925081723	24.805620081733952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	289.0
1	289.0
2	607.5
3	926.0
4	926.0
5	926.0
6	2467.5
7	4009.0
8	4009.0
9	4009.0
10	4035.0
11	4061.0
12	4061.0
13	4061.0
14	4330.5
15	4600.0
16	4600.0
17	4600.0
18	7198.5
19	9797.0
20	9797.0
21	9797.0
22	16169.5
23	22542.0
24	22542.0
25	22542.0
26	33720.5
27	44899.0
28	44899.0
29	44899.0
30	56210.0
31	67521.0
32	67521.0
33	67521.0
34	79791.5
35	92062.0
36	92062.0
37	92062.0
38	102322.0
39	112582.0
40	112582.0
41	112582.0
42	126466.5
43	140351.0
44	140351.0
45	140351.0
46	161895.5
47	183440.0
48	183440.0
49	183440.0
50	183841.5
51	184243.0
52	184243.0
53	184243.0
54	171347.0
55	158451.0
56	158451.0
57	158451.0
58	145437.0
59	132423.0
60	132423.0
61	132423.0
62	117393.5
63	102364.0
64	102364.0
65	102364.0
66	84613.5
67	66863.0
68	66863.0
69	66863.0
70	50783.5
71	34704.0
72	34704.0
73	34704.0
74	26860.0
75	19016.0
76	19016.0
77	19016.0
78	15490.0
79	11964.0
80	11964.0
81	11964.0
82	8352.5
83	4741.0
84	4741.0
85	4741.0
86	3488.0
87	2235.0
88	2235.0
89	2235.0
90	1526.5
91	818.0
92	818.0
93	818.0
94	492.5
95	167.0
96	167.0
97	167.0
98	399.0
99	631.0
100	631.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010457430787103072
2	0.0039126441720453665
3	0.005975674735487469
4	0.015650576688181466
5	0.0376325230365818
6	0.047876536868846034
7	0.06928937133767613
8	0.09034651088177484
9	0.09831407719575812
10	0.12008260658931962
11	0.12406638974631126
12	0.13374129169900525
13	0.12321272192695591
14	0.12584486436996825
15	0.11624110140222053
16	0.11296870809469169
17	0.12271474903233195
18	0.13103801027104664
19	0.13203395606029456
20	0.12150538628824521
21	0.13132256621083177
22	0.1535890684990172
23	0.14000152237427785
24	0.13601773921728622
25	0.13345673575922015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1405699.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.9678435566604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11567024888656	41.11470774374469
2	12.344510422843689	12.83035172875809
3	3.7044376685777385	5.775349116781432
4	1.542174423414343	3.205739166923182
5	0.8309143759211398	2.1590414148424957
6	0.4970403239642383	1.5498068278275206
7	0.33557253048282903	1.220728653623102
8	0.24052417572117774	0.9999618188378289
9	0.18083176001042436	0.8457692952867564
>10	0.9842310226324357	9.486616627114982
>50	0.10457592753901378	3.7759253415274654
>100	0.10799080999068889	11.800051235077587
>500	0.008781950488095111	3.129547882745362
>1k	0.002744359527529722	2.106403146909455
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3130	0.2226650228818545	No Hit
GTACATGGAAGCAGTGGTATCAACG	2226	0.15835538049041792	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2198	0.15636348891192212	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2086	0.14839592259793882	No Hit
TCCATGTACTCTGCGTTGATACCAC	1933	0.13751165790115807	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1511	0.10749100625382817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.422779698925588E-4
7	0.0	0.0	0.0	0.0	1.422779698925588E-4
8	0.0	7.11389849462794E-5	0.0	0.0	2.134169548388382E-4
9	0.0	7.11389849462794E-5	0.0	0.0	2.134169548388382E-4
10	0.0	7.11389849462794E-5	0.0	7.11389849462794E-5	2.134169548388382E-4
11	0.0	7.11389849462794E-5	0.0	7.11389849462794E-5	2.134169548388382E-4
12	0.0	7.11389849462794E-5	0.0	7.11389849462794E-5	3.5569492473139697E-4
13	0.0	7.11389849462794E-5	0.0	7.11389849462794E-5	3.5569492473139697E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	135	0.0	15.472368	2
GCGTTAT	130	0.0	15.336028	1
GTCTTAA	55	1.9709437E-4	13.809063	1
AAGGCGT	65	5.447241E-5	13.155006	6
TCCCCGA	175	0.0	13.02972	13
TGTGCGA	60	4.087315E-4	12.668235	10
GTCCTAA	345	0.0	12.383127	1
CGCGTAT	85	3.9386578E-6	12.295201	7
ACGCGTA	85	3.9386578E-6	12.295201	6
GTTTAGA	85	3.971745E-6	12.286004	1
TAACCTG	110	3.8131475E-8	12.090683	5
ATCCCCG	190	0.0	12.001485	12
CGTCTTA	145	9.640644E-11	11.794984	15
TGGCGAG	605	0.0	11.778738	18
GCATCGC	210	0.0	11.762941	14
TCTAGGG	105	2.7296846E-7	11.758331	3
CGCGGTC	130	2.6084308E-9	11.693756	10
CGCATCG	205	0.0	11.586387	13
ATCGTAA	140	6.87578E-10	11.528102	1
CGTCGTA	200	0.0	11.401411	10
>>END_MODULE