##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062661_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1430
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.192307692307693	32.0	32.0	32.0	21.0	32.0
2	28.988811188811187	32.0	32.0	32.0	14.0	32.0
3	29.154545454545456	32.0	32.0	32.0	14.0	32.0
4	28.698601398601397	32.0	32.0	32.0	14.0	32.0
5	28.54965034965035	32.0	32.0	32.0	14.0	32.0
6	32.17062937062937	36.0	32.0	36.0	14.0	36.0
7	32.158041958041956	36.0	32.0	36.0	14.0	36.0
8	32.08811188811189	36.0	32.0	36.0	14.0	36.0
9	32.72867132867133	36.0	32.0	36.0	21.0	36.0
10	31.377622377622377	36.0	32.0	36.0	14.0	36.0
11	32.625174825174824	36.0	32.0	36.0	21.0	36.0
12	31.79090909090909	36.0	32.0	36.0	14.0	36.0
13	32.315384615384616	36.0	32.0	36.0	14.0	36.0
14	32.11048951048951	36.0	32.0	36.0	14.0	36.0
15	31.579020979020978	36.0	32.0	36.0	14.0	36.0
16	31.82937062937063	36.0	32.0	36.0	14.0	36.0
17	31.55944055944056	36.0	32.0	36.0	14.0	36.0
18	31.986713286713286	36.0	32.0	36.0	14.0	36.0
19	32.13776223776224	36.0	32.0	36.0	14.0	36.0
20	31.8027972027972	36.0	32.0	36.0	14.0	36.0
21	31.80979020979021	36.0	32.0	36.0	14.0	36.0
22	31.63986013986014	36.0	32.0	36.0	14.0	36.0
23	31.62097902097902	36.0	32.0	36.0	14.0	36.0
24	31.55944055944056	36.0	32.0	36.0	14.0	36.0
25	30.799300699300698	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	4.0
17	10.0
18	8.0
19	5.0
20	11.0
21	33.0
22	18.0
23	26.0
24	35.0
25	32.0
26	55.0
27	44.0
28	41.0
29	76.0
30	86.0
31	98.0
32	144.0
33	145.0
34	265.0
35	290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86713286713287	18.041958041958043	10.76923076923077	28.321678321678323
2	16.083916083916083	19.51048951048951	39.37062937062937	25.034965034965033
3	19.72027972027972	23.356643356643357	28.391608391608393	28.53146853146853
4	12.587412587412588	15.454545454545453	35.94405594405594	36.01398601398601
5	14.055944055944055	35.66433566433567	33.98601398601399	16.293706293706293
6	34.21973407977607	35.61931420573828	17.704688593421974	12.45626312106368
7	29.531140657802656	30.230930720783768	20.85374387683695	19.384184744576626
8	26.941917424772566	33.939818054583625	19.52414275717285	19.59412176347096
9	28.621413575927225	13.925822253324002	17.774667599720082	39.67809657102869
10	16.864940517844644	26.452064380685798	28.691392582225333	27.991602519244225
11	34.35969209237229	20.713785864240727	22.673198040587824	22.25332400279916
12	25.192442267319805	22.953114065780266	31.00069979006298	20.85374387683695
13	28.761371588523442	19.52414275717285	25.192442267319805	26.522043386983906
14	23.932820153953813	19.59412176347096	25.052484254723584	31.420573827851644
15	23.862841147655704	27.01189643107068	23.023093072078378	26.102169349195243
16	25.962211336599022	27.921623512946113	22.953114065780266	23.163051084674596
17	22.043386983904828	26.38208537438768	27.641707487753674	23.932820153953813
18	23.093072078376487	26.452064380685798	28.621413575927225	21.833449965010495
19	23.862841147655704	24.002799160251925	29.391182645206438	22.743177046885936
20	28.691392582225333	22.18334499650105	24.282715185444367	24.84254723582925
21	28.551434569629112	25.40237928621414	22.533240027991603	23.51294611616515
22	25.40237928621414	25.052484254723584	25.472358292512247	24.072778166550034
23	24.002799160251925	23.862841147655704	26.312106368089573	25.822253324002798
24	23.792862141357592	23.792862141357592	27.641707487753674	24.772568229531142
25	23.30300909727082	24.56263121063681	26.662001399580127	25.472358292512247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.5
7	5.0
8	5.0
9	5.0
10	4.0
11	3.0
12	3.0
13	3.0
14	4.0
15	5.0
16	5.0
17	5.0
18	10.0
19	15.0
20	15.0
21	15.0
22	14.0
23	13.0
24	13.0
25	13.0
26	35.5
27	58.0
28	58.0
29	58.0
30	63.5
31	69.0
32	69.0
33	69.0
34	85.0
35	101.0
36	101.0
37	101.0
38	109.5
39	118.0
40	118.0
41	118.0
42	123.0
43	128.0
44	128.0
45	128.0
46	156.0
47	184.0
48	184.0
49	184.0
50	189.0
51	194.0
52	194.0
53	194.0
54	179.0
55	164.0
56	164.0
57	164.0
58	151.0
59	138.0
60	138.0
61	138.0
62	118.0
63	98.0
64	98.0
65	98.0
66	79.5
67	61.0
68	61.0
69	61.0
70	46.0
71	31.0
72	31.0
73	31.0
74	27.0
75	23.0
76	23.0
77	23.0
78	15.0
79	7.0
80	7.0
81	7.0
82	6.5
83	6.0
84	6.0
85	6.0
86	4.5
87	3.0
88	3.0
89	3.0
90	2.0
91	1.0
92	1.0
93	1.0
94	1.5
95	2.0
96	2.0
97	2.0
98	2.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.06993006993006994
7	0.06993006993006994
8	0.06993006993006994
9	0.06993006993006994
10	0.06993006993006994
11	0.06993006993006994
12	0.06993006993006994
13	0.06993006993006994
14	0.06993006993006994
15	0.06993006993006994
16	0.06993006993006994
17	0.06993006993006994
18	0.06993006993006994
19	0.06993006993006994
20	0.06993006993006994
21	0.06993006993006994
22	0.06993006993006994
23	0.06993006993006994
24	0.06993006993006994
25	0.06993006993006994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1430.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.84615384615384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.93293591654248	89.0909090909091
2	4.023845007451564	7.5524475524475525
3	0.6706408345752608	1.8881118881118881
4	0.29806259314456035	1.118881118881119
5	0.07451564828614009	0.34965034965034963
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5	0.34965034965034963	No Hit
GTATCTGATCGTCTTCGAACCTCCG	4	0.27972027972027974	No Hit
GTACAGTGAAACTGCGAATGGCTCA	4	0.27972027972027974	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4	0.27972027972027974	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4	0.27972027972027974	No Hit
TCCATGTACTCTGCGTTGATACCAC	3	0.2097902097902098	No Hit
GTATCAACGCAGAGTACATGGAAGC	3	0.2097902097902098	No Hit
CCATTGGAGGGCAAGTCTGGTGCCA	3	0.2097902097902098	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	3	0.2097902097902098	No Hit
GTATTGCGCCGCTAGAGGTGAAATT	3	0.2097902097902098	No Hit
CAGTTATCCAAGTAGGAGAGGAGCG	3	0.2097902097902098	No Hit
CTCTAATTTTTTCAAAGTAAACGCT	3	0.2097902097902098	No Hit
TCGTAGTTCCGACCATAAACGATGC	3	0.2097902097902098	No Hit
ATTGGAATGAGTCCACTTTAAATCC	3	0.2097902097902098	No Hit
GCGAATGGCTCATTAAATCAGTTAT	2	0.13986013986013987	No Hit
GTATTGTTATTTTTCGTCACTACCT	2	0.13986013986013987	No Hit
GTATCAACGCAGAGTACATGGGCTT	2	0.13986013986013987	No Hit
TAACAATACAGGACTCTTTCGAGGC	2	0.13986013986013987	No Hit
GTATCAACGCAGAGTACATGGGGTA	2	0.13986013986013987	No Hit
GAATAATTGCAATCCCCGATCCCCA	2	0.13986013986013987	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2	0.13986013986013987	No Hit
AGCTTGGTGATAGCTGGTTACCCAA	2	0.13986013986013987	No Hit
CCCACGGAATCGAGAAAGAGCTATC	2	0.13986013986013987	No Hit
CCATTATTCCTAGCTGCGGTATCCA	2	0.13986013986013987	No Hit
CTCTAGATAACCTCGGGCCGATCGC	2	0.13986013986013987	No Hit
GGCCCGAGCCGCCTGGATACCGCAG	2	0.13986013986013987	No Hit
GTTGAACCCCATTCGTGATGGGGAT	2	0.13986013986013987	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2	0.13986013986013987	No Hit
ACACTGACTGGCTCAGCGTGTGCCT	2	0.13986013986013987	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2	0.13986013986013987	No Hit
GGTATCAACGCAGAGTACATGGGAA	2	0.13986013986013987	No Hit
ACTTCTTAGAGGGACAAGTGGCGTT	2	0.13986013986013987	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	2	0.13986013986013987	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2	0.13986013986013987	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	2	0.13986013986013987	No Hit
CTATTTCATCATCTCCCACCCAGCT	2	0.13986013986013987	No Hit
TGCCAAGAATGTTTTCATTAATCAA	2	0.13986013986013987	No Hit
GGTGACTCTAGATAACCTCGGGCCG	2	0.13986013986013987	No Hit
CTCCAATAGCGTATATTAAAGTTGC	2	0.13986013986013987	No Hit
ATTGATAGCTCTTTCTCGATTCCGT	2	0.13986013986013987	No Hit
ATAAATAATCCACCTATAACTTCTC	2	0.13986013986013987	No Hit
CTAGTTACGCGACCCCCGAGCGGTC	2	0.13986013986013987	No Hit
GTTTAGAAAACATCCCAGAAAAATG	2	0.13986013986013987	No Hit
GTGGTATCAACGCAGAGTACATGGA	2	0.13986013986013987	No Hit
AAGGTATGCTGTGTGTGAGCAACAA	2	0.13986013986013987	No Hit
TATCCAAGTAGGAGAGGAGCGAGCG	2	0.13986013986013987	No Hit
ATTCCTAGCTGCGGTATCCAGGCGG	2	0.13986013986013987	No Hit
CCTCTGTGGTCTCTTCACTAGCTCC	2	0.13986013986013987	No Hit
GTTCTACAGTGAGCACCCAGGCTCA	2	0.13986013986013987	No Hit
CAGATGCTCCTTGCTGCTCCTGGTA	2	0.13986013986013987	No Hit
TTCCCGTGTTGGGTCAAATTAAGCC	2	0.13986013986013987	No Hit
GTCCTGAGAGATGGGCGAGTGCCGT	2	0.13986013986013987	No Hit
CCCTCTCCGGAATCGAACCCTGATT	2	0.13986013986013987	No Hit
CCAATGGATCCTCGTTAAAGGATTT	2	0.13986013986013987	No Hit
CGGCCGGTACAGTGAAACTGCGAAT	2	0.13986013986013987	No Hit
GTACATGGGTGGTATCAACGCAAAA	2	0.13986013986013987	No Hit
GTATTAGCTCTAGAATTACCACAGT	2	0.13986013986013987	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	2	0.13986013986013987	No Hit
GTACATGGGCTTTCTGCTCTTTGAC	2	0.13986013986013987	No Hit
CCCCGGAACCCAAAGACTTTGGTTT	2	0.13986013986013987	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	2	0.13986013986013987	No Hit
CTCCTACTTGGATAACTGTGGTAAT	2	0.13986013986013987	No Hit
GTACATGGAAGCAGTGGTATCAACG	2	0.13986013986013987	No Hit
GCTCGGGCCTGCTTTGAACACTCTA	2	0.13986013986013987	No Hit
AGCTAATACATGCCGACGGGCGCTG	2	0.13986013986013987	No Hit
GTTTCTAGCTGTAATTCTTTTAGTT	2	0.13986013986013987	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2	0.13986013986013987	No Hit
CACCAGGAGTGGAGCCTGCGGCTTA	2	0.13986013986013987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE