##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1430
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.46993006993007	32.0	32.0	32.0	21.0	32.0
2	30.148951048951048	32.0	32.0	32.0	21.0	32.0
3	30.754545454545454	32.0	32.0	32.0	32.0	32.0
4	31.155944055944055	32.0	32.0	32.0	32.0	32.0
5	31.068531468531468	32.0	32.0	32.0	32.0	32.0
6	34.5979020979021	36.0	36.0	36.0	32.0	36.0
7	34.757342657342654	36.0	36.0	36.0	32.0	36.0
8	34.46573426573426	36.0	36.0	36.0	32.0	36.0
9	34.76783216783217	36.0	36.0	36.0	32.0	36.0
10	34.52167832167832	36.0	36.0	36.0	32.0	36.0
11	34.955244755244756	36.0	36.0	36.0	32.0	36.0
12	34.886013986013985	36.0	36.0	36.0	32.0	36.0
13	34.90839160839161	36.0	36.0	36.0	36.0	36.0
14	34.70839160839161	36.0	36.0	36.0	32.0	36.0
15	34.602797202797206	36.0	36.0	36.0	32.0	36.0
16	34.84965034965035	36.0	36.0	36.0	32.0	36.0
17	34.746153846153845	36.0	36.0	36.0	32.0	36.0
18	34.71818181818182	36.0	36.0	36.0	32.0	36.0
19	34.58881118881119	36.0	36.0	36.0	32.0	36.0
20	34.74965034965035	36.0	36.0	36.0	32.0	36.0
21	34.66433566433567	36.0	36.0	36.0	32.0	36.0
22	34.55734265734266	36.0	36.0	36.0	32.0	36.0
23	34.55734265734266	36.0	36.0	36.0	32.0	36.0
24	34.652447552447555	36.0	36.0	36.0	32.0	36.0
25	34.462937062937065	36.0	36.0	36.0	32.0	36.0
26	34.512587412587415	36.0	36.0	36.0	32.0	36.0
27	34.24545454545454	36.0	36.0	36.0	32.0	36.0
28	34.303496503496504	36.0	36.0	36.0	32.0	36.0
29	34.48881118881119	36.0	36.0	36.0	32.0	36.0
30	34.466433566433565	36.0	36.0	36.0	32.0	36.0
31	34.344055944055945	36.0	36.0	36.0	32.0	36.0
32	34.14055944055944	36.0	36.0	36.0	32.0	36.0
33	34.246153846153845	36.0	36.0	36.0	32.0	36.0
34	34.26083916083916	36.0	36.0	36.0	32.0	36.0
35	34.137062937062936	36.0	36.0	36.0	32.0	36.0
36	34.21468531468531	36.0	36.0	36.0	32.0	36.0
37	34.18951048951049	36.0	36.0	36.0	32.0	36.0
38	34.065734265734264	36.0	36.0	36.0	32.0	36.0
39	34.17132867132867	36.0	36.0	36.0	32.0	36.0
40	34.07902097902098	36.0	36.0	36.0	32.0	36.0
41	34.17762237762238	36.0	36.0	36.0	32.0	36.0
42	34.04545454545455	36.0	36.0	36.0	32.0	36.0
43	33.6986013986014	36.0	36.0	36.0	32.0	36.0
44	34.025874125874125	36.0	36.0	36.0	32.0	36.0
45	33.878321678321676	36.0	36.0	36.0	32.0	36.0
46	33.67412587412588	36.0	36.0	36.0	27.0	36.0
47	33.78671328671329	36.0	36.0	36.0	32.0	36.0
48	33.91398601398601	36.0	36.0	36.0	32.0	36.0
49	33.792307692307695	36.0	36.0	36.0	32.0	36.0
50	33.12657342657343	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	2.0
25	12.0
26	7.0
27	14.0
28	16.0
29	33.0
30	56.0
31	69.0
32	105.0
33	165.0
34	284.0
35	666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47552447552447	16.643356643356643	11.958041958041958	26.923076923076923
2	17.762237762237763	19.79020979020979	36.08391608391609	26.36363636363636
3	19.58041958041958	22.937062937062937	30.13986013986014	27.342657342657343
4	14.895104895104897	15.664335664335665	37.20279720279721	32.23776223776223
5	17.552447552447553	33.00699300699301	33.63636363636363	15.804195804195803
6	34.89510489510489	35.10489510489511	16.853146853146853	13.146853146853147
7	28.74125874125874	30.55944055944056	19.93006993006993	20.76923076923077
8	30.349650349650346	33.14685314685315	17.062937062937063	19.44055944055944
9	26.013986013986013	14.195804195804197	20.909090909090907	38.88111888111888
10	17.622377622377623	24.545454545454547	29.790209790209794	28.041958041958043
11	37.06293706293706	19.79020979020979	22.447552447552447	20.6993006993007
12	23.006993006993007	23.776223776223777	28.391608391608393	24.825174825174827
13	29.58041958041958	18.53146853146853	25.874125874125873	26.013986013986013
14	22.587412587412587	19.72027972027972	24.055944055944057	33.63636363636363
15	25.734265734265733	28.18181818181818	22.097902097902097	23.986013986013983
16	25.664335664335663	24.125874125874127	24.965034965034967	25.244755244755247
17	22.867132867132867	25.874125874125873	26.083916083916087	25.174825174825177
18	24.825174825174827	24.615384615384617	26.223776223776223	24.335664335664337
19	25.944055944055943	23.916083916083917	25.384615384615383	24.755244755244753
20	26.573426573426573	24.335664335664337	23.216783216783217	25.874125874125873
21	27.762237762237763	23.426573426573427	24.055944055944057	24.755244755244753
22	25.524475524475527	23.566433566433567	27.62237762237762	23.286713286713287
23	24.545454545454547	23.636363636363637	26.923076923076923	24.895104895104893
24	24.895104895104893	25.454545454545453	24.335664335664337	25.314685314685313
25	23.006993006993007	25.664335664335663	24.755244755244753	26.573426573426573
26	23.636363636363637	23.986013986013983	26.153846153846157	26.223776223776223
27	23.706293706293707	23.986013986013983	27.27272727272727	25.034965034965033
28	24.615384615384617	23.216783216783217	26.36363636363636	25.804195804195807
29	26.293706293706293	23.496503496503497	25.944055944055943	24.265734265734267
30	21.74825174825175	23.496503496503497	29.09090909090909	25.664335664335663
31	24.335664335664337	23.916083916083917	25.454545454545453	26.293706293706293
32	23.986013986013983	25.874125874125873	25.314685314685313	24.825174825174827
33	23.496503496503497	23.846153846153847	26.713286713286717	25.944055944055943
34	26.013986013986013	25.734265734265733	27.342657342657343	20.909090909090907
35	25.104895104895103	24.265734265734267	26.36363636363636	24.265734265734267
36	25.384615384615383	24.545454545454547	26.713286713286717	23.356643356643357
37	24.965034965034967	25.384615384615383	22.727272727272727	26.923076923076923
38	24.545454545454547	25.454545454545453	25.104895104895103	24.895104895104893
39	26.293706293706293	23.916083916083917	25.174825174825177	24.615384615384617
40	25.244755244755247	25.804195804195807	25.594405594405593	23.356643356643357
41	23.566433566433567	25.524475524475527	25.454545454545453	25.454545454545453
42	25.454545454545453	23.776223776223777	26.083916083916087	24.685314685314687
43	24.615384615384617	23.426573426573427	26.083916083916087	25.874125874125873
44	24.755244755244753	24.125874125874127	26.783216783216783	24.335664335664337
45	26.433566433566437	25.174825174825177	23.566433566433567	24.825174825174827
46	23.916083916083917	24.825174825174827	27.482517482517483	23.776223776223777
47	24.335664335664337	25.104895104895103	27.062937062937063	23.496503496503497
48	24.755244755244753	23.916083916083917	25.454545454545453	25.874125874125873
49	25.454545454545453	23.286713286713287	25.244755244755247	26.013986013986013
50	22.30769230769231	26.153846153846157	25.594405594405593	25.944055944055943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	1.5
12	2.0
13	2.0
14	2.0
15	2.5
16	3.0
17	2.5
18	2.0
19	3.5
20	5.0
21	5.5
22	6.0
23	6.0
24	6.0
25	9.5
26	13.0
27	13.5
28	14.0
29	19.5
30	25.0
31	29.5
32	34.0
33	31.5
34	29.0
35	35.0
36	41.0
37	51.5
38	62.0
39	65.0
40	68.0
41	70.5
42	73.0
43	74.5
44	76.0
45	81.5
46	87.0
47	94.0
48	101.0
49	102.0
50	103.0
51	90.5
52	78.0
53	79.5
54	81.0
55	96.0
56	111.0
57	102.0
58	93.0
59	86.5
60	80.0
61	71.0
62	62.0
63	51.5
64	41.0
65	36.0
66	31.0
67	27.0
68	23.0
69	23.5
70	24.0
71	17.5
72	11.0
73	13.0
74	15.0
75	9.5
76	4.0
77	2.5
78	1.0
79	2.5
80	4.0
81	2.5
82	1.0
83	1.5
84	2.0
85	3.0
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1430.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.03496503496504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.95290654893304	91.18881118881119
2	3.164091243561442	6.013986013986014
3	0.7358351729212657	2.097902097902098
4	0.07358351729212656	0.27972027972027974
5	0.0	0.0
6	0.07358351729212656	0.4195804195804196
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6	0.4195804195804196	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG	4	0.27972027972027974	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3	0.2097902097902098	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3	0.2097902097902098	No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA	3	0.2097902097902098	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3	0.2097902097902098	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC	3	0.2097902097902098	No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC	3	0.2097902097902098	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3	0.2097902097902098	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	3	0.2097902097902098	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	3	0.2097902097902098	No Hit
GCTTCATTTTGGCCAACTCCCGTCTCGTGTGCTCGTCATTCCGAAGGTCC	3	0.2097902097902098	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	2	0.13986013986013987	No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA	2	0.13986013986013987	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC	2	0.13986013986013987	No Hit
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGT	2	0.13986013986013987	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2	0.13986013986013987	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAA	2	0.13986013986013987	No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC	2	0.13986013986013987	No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC	2	0.13986013986013987	No Hit
ACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT	2	0.13986013986013987	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG	2	0.13986013986013987	No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC	2	0.13986013986013987	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG	2	0.13986013986013987	No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA	2	0.13986013986013987	No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAA	2	0.13986013986013987	No Hit
GTTCCATATATCAGTTCACCACCAAAAGCAGTGAGGGCAGAAACCTGAAG	2	0.13986013986013987	No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCA	2	0.13986013986013987	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2	0.13986013986013987	No Hit
CCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGC	2	0.13986013986013987	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	2	0.13986013986013987	No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA	2	0.13986013986013987	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2	0.13986013986013987	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT	2	0.13986013986013987	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA	2	0.13986013986013987	No Hit
GGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC	2	0.13986013986013987	No Hit
ACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGAC	2	0.13986013986013987	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	2	0.13986013986013987	No Hit
GGATATTCTTCCTTAATGACATCAGATGCTTCCTCAAAAATTGGATGCAA	2	0.13986013986013987	No Hit
GGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCT	2	0.13986013986013987	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT	2	0.13986013986013987	No Hit
CACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGAT	2	0.13986013986013987	No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA	2	0.13986013986013987	No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA	2	0.13986013986013987	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	2	0.13986013986013987	No Hit
TCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAA	2	0.13986013986013987	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	2	0.13986013986013987	No Hit
CGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGG	2	0.13986013986013987	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2	0.13986013986013987	No Hit
GAAAAGGACCGTCCCCATGAGGGGACTCGGCCAGTGCACGCTGTGTTTGT	2	0.13986013986013987	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	2	0.13986013986013987	No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2	0.13986013986013987	No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG	2	0.13986013986013987	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC	2	0.13986013986013987	No Hit
ATTTATAACCATAGGGCCGATGACAATCGGACATGCTCTTTCATAACAAG	2	0.13986013986013987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.13986013986013987	0.0
33	0.0	0.0	0.0	0.2097902097902098	0.0
34	0.0	0.0	0.0	0.27972027972027974	0.0
35	0.0	0.0	0.0	0.48951048951048953	0.0
36	0.0	0.0	0.0	0.5594405594405595	0.0
37	0.0	0.0	0.0	0.6993006993006993	0.0
38	0.0	0.0	0.0	1.048951048951049	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE