FastQCFastQC Report
Thu 2 Feb 2017
SRR4062660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050483
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG71900.3506490909702738No Hit
TCCATGTACTCTGCGTTGATACCAC60790.2964667349107503No Hit
GTATCAACGCAGAGTACTTTTTTTT52280.25496431816308646No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41690.20331794996593486No Hit
TATCAACGCAGAGTACTTTTTTTTT37840.18454188598491184No Hit
GAGTACATGGAAGCAGTGGTATCAA31470.1534760346708556No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30730.1498671288667109No Hit
CATGTACTCTGCGTTGATACCACTG29620.1444537701604939No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25180.1228003353356258No Hit
GTCTTGCGCCGGTCCAAGAATTTCA24060.11733820763205546No Hit
GAATAGGACCGCGGTTCTATTTTGT23950.1168017486611691No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23610.11514360275115669No Hit
GCGCAAGACGGACCAGAGCGAAAGC23580.11499729575909677No Hit
GAATAACGCCGCCGCATCGCCAGTC22020.10738933217198095No Hit
GTATCTGATCGTCTTCGAACCTCCG21950.1070479491905078No Hit
GAACTACGACGGTATCTGATCGTCT20720.1010493625160511No Hit
CCCATGTACTCTGCGTTGATACCAC20640.10065921053722465No Hit
GTCCTATTCCATTATTCCTAGCTGC20640.10065921053722465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1250.014.4446616
AACCGGT602.5671481E-514.25111714
AATCGTA400.00527797114.2507713
GCGTTAT1950.014.1189671
AAGACGG4950.013.4327725
CAAGACG5050.013.3545484
TCGCGTA1850.013.3520739
CGTTATT2050.012.96744252
CGCCAGT4000.012.82976518
GCGTAAC2000.012.82506711
CGCGCCA905.4058364E-712.66704210
CGCAAGA4550.012.51958752
GTTATTC2500.012.1556093
AGGCGTA951.0396197E-612.000648512
GCAAGAC5950.011.9704673
ACGGTAT3900.011.9365429
ACGAACG1603.6379788E-1211.87564115
ATCGCCA4300.011.71308116
TCCAACG1552.5465852E-1111.64948818
CTAGACA1552.5465852E-1111.6435164
Produced by FastQC (version 0.11.5)