##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062660_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2050483
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.119170946552593	32.0	32.0	32.0	32.0	32.0
2	30.780441486225442	32.0	32.0	32.0	32.0	32.0
3	30.77522808040837	32.0	32.0	32.0	32.0	32.0
4	30.838361985932096	32.0	32.0	32.0	32.0	32.0
5	30.637794607416886	32.0	32.0	32.0	32.0	32.0
6	34.29187513380994	36.0	36.0	36.0	32.0	36.0
7	34.150398710937864	36.0	36.0	36.0	32.0	36.0
8	34.141293539132	36.0	36.0	36.0	32.0	36.0
9	34.29880374526392	36.0	36.0	36.0	32.0	36.0
10	33.96000600834047	36.0	36.0	36.0	32.0	36.0
11	34.31040979125406	36.0	36.0	36.0	32.0	36.0
12	34.073509022020666	36.0	36.0	36.0	32.0	36.0
13	34.20070637015767	36.0	36.0	36.0	32.0	36.0
14	34.1097604808233	36.0	36.0	36.0	32.0	36.0
15	34.04719717256861	36.0	36.0	36.0	32.0	36.0
16	34.04674313320325	36.0	36.0	36.0	32.0	36.0
17	33.96345885335309	36.0	36.0	36.0	32.0	36.0
18	33.99013500721537	36.0	36.0	36.0	32.0	36.0
19	33.970584979246354	36.0	36.0	36.0	32.0	36.0
20	33.95310080600522	36.0	36.0	36.0	32.0	36.0
21	33.91786666848738	36.0	36.0	36.0	32.0	36.0
22	33.874808520724145	36.0	36.0	36.0	32.0	36.0
23	33.835923048374454	36.0	36.0	36.0	32.0	36.0
24	33.80577454190062	36.0	36.0	36.0	32.0	36.0
25	33.33096104673874	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	65.0
5	214.0
6	330.0
7	97.0
8	307.0
9	400.0
10	236.0
11	81.0
12	166.0
13	119.0
14	435.0
15	550.0
16	757.0
17	1074.0
18	1455.0
19	1986.0
20	3142.0
21	4566.0
22	6871.0
23	9592.0
24	13375.0
25	18683.0
26	25345.0
27	32962.0
28	43692.0
29	57845.0
30	74485.0
31	102162.0
32	146063.0
33	211480.0
34	462382.0
35	829561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.41921923445158	17.054646565098828	10.780165642355515	25.745968558094074
2	16.578099532681563	20.065966908275467	37.27027899622614	26.085654562816824
3	19.567684565116775	24.076851308597686	28.047196621538912	28.30826750474663
4	12.442468466183456	14.902350037557676	35.66813645631115	36.987045039947716
5	14.396228265276203	36.77543806070233	33.5934759605088	15.234857713512667
6	34.55556493427604	34.92140008792144	16.908580732324108	13.614454245478413
7	30.054829083083018	30.38077785558991	20.93285084390914	18.631542217417934
8	28.31386051815131	32.82889174819932	19.737263431654128	19.119984301995245
9	27.251527249330472	14.12318753557561	18.77332856876454	39.85195664632938
10	15.860954023403817	26.680263393035887	31.72102915858171	25.73775342497859
11	37.51797480963181	20.977536675935852	22.134125649107304	19.370362865325035
12	24.47447711412903	23.625553651044797	29.191901434243107	22.708067800583073
13	29.718821182772338	19.30170386990139	25.52381975453922	25.45565519278706
14	23.368877890335	19.903942779741264	25.100515345619463	31.626663984304276
15	25.10354511725511	27.528622297000062	22.822354515631492	24.545478070113337
16	25.63625462488734	25.68053831784462	24.593659278239276	24.089547779028766
17	23.61022337696835	25.994678640782865	25.920068282150204	24.475029700098585
18	24.84864495852691	24.495198341271802	27.304166255663304	23.351990444537986
19	25.148453737127497	25.525789930148612	25.323277545981593	24.0024787867423
20	25.152356626567208	24.3238373606134	26.027897583534305	24.49590842928509
21	26.24192923017723	24.506036169159234	24.3977748065994	24.85425979406413
22	25.112326211240195	24.971317070311642	25.19477284438442	24.721583874063743
23	23.676568694770545	24.644289834801235	25.759208124266824	25.919933346161393
24	24.235408333239732	25.6562520296895	25.593401854837094	24.514937782233666
25	24.654851128912224	24.811213138706094	25.4337800540284	25.100155678353286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	403.0
1	403.0
2	926.0
3	1449.0
4	1449.0
5	1449.0
6	3891.0
7	6333.0
8	6333.0
9	6333.0
10	6447.5
11	6562.0
12	6562.0
13	6562.0
14	6324.5
15	6087.0
16	6087.0
17	6087.0
18	9938.0
19	13789.0
20	13789.0
21	13789.0
22	22501.5
23	31214.0
24	31214.0
25	31214.0
26	46480.0
27	61746.0
28	61746.0
29	61746.0
30	77577.5
31	93409.0
32	93409.0
33	93409.0
34	111358.5
35	129308.0
36	129308.0
37	129308.0
38	145456.5
39	161605.0
40	161605.0
41	161605.0
42	185876.5
43	210148.0
44	210148.0
45	210148.0
46	248907.0
47	287666.0
48	287666.0
49	287666.0
50	280493.5
51	273321.0
52	273321.0
53	273321.0
54	248666.0
55	224011.0
56	224011.0
57	224011.0
58	206764.0
59	189517.0
60	189517.0
61	189517.0
62	168673.5
63	147830.0
64	147830.0
65	147830.0
66	123210.5
67	98591.0
68	98591.0
69	98591.0
70	73883.0
71	49175.0
72	49175.0
73	49175.0
74	38364.5
75	27554.0
76	27554.0
77	27554.0
78	22738.5
79	17923.0
80	17923.0
81	17923.0
82	12607.0
83	7291.0
84	7291.0
85	7291.0
86	5279.5
87	3268.0
88	3268.0
89	3268.0
90	2222.0
91	1176.0
92	1176.0
93	1176.0
94	700.5
95	225.0
96	225.0
97	225.0
98	553.5
99	882.0
100	882.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007900577571235655
2	0.0029749088385516973
3	0.003999057782971134
4	0.014777006198051872
5	0.03462598812084763
6	0.045160091549161833
7	0.06769136832638943
8	0.08851573019625132
9	0.09895229562985892
10	0.11904512253942119
11	0.12084957544149355
12	0.1323102898195206
13	0.12182495538855967
14	0.12372694628533863
15	0.11421699180144385
16	0.11338791884643765
17	0.12241018335679935
18	0.12918907398890897
19	0.13201767583540075
20	0.12153234140443983
21	0.12977430195714865
22	0.15108635380054358
23	0.14089363335370253
24	0.13440735670571274
25	0.1302619919306817
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2050483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.79768673447082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2522566721119	32.082793903182065
2	14.773949309595816	12.941296073852582
3	5.085536403348264	6.682041908117843
4	2.2803756825806625	3.9950071913029177
5	1.2346537426176252	2.703748892235436
6	0.7365758540368484	1.935619110676872
7	0.485836236878199	1.4894952314931866
8	0.3232791623875715	1.1327103585626381
9	0.23408754899337478	0.9227243825326729
>10	1.3519713395235877	10.74591796380369
>50	0.10948744146391208	3.3316432733481847
>100	0.10642026510441996	10.028016667940916
>500	0.016637471014507296	4.987906736903959
>1k	0.008597887705483635	6.116484701524053
>5k	3.349826378759858E-4	0.9045936045230265
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7190	0.3506490909702738	No Hit
TCCATGTACTCTGCGTTGATACCAC	6079	0.2964667349107503	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5228	0.25496431816308646	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4169	0.20331794996593486	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3784	0.18454188598491184	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3147	0.1534760346708556	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3073	0.1498671288667109	No Hit
CATGTACTCTGCGTTGATACCACTG	2962	0.1444537701604939	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2518	0.1228003353356258	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2406	0.11733820763205546	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2395	0.1168017486611691	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2361	0.11514360275115669	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2358	0.11499729575909677	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2202	0.10738933217198095	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2195	0.1070479491905078	No Hit
GAACTACGACGGTATCTGATCGTCT	2072	0.1010493625160511	No Hit
CCCATGTACTCTGCGTTGATACCAC	2064	0.10065921053722465	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2064	0.10065921053722465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.753799470661303E-5
2	0.0	0.0	0.0	0.0	9.753799470661303E-5
3	0.0	0.0	0.0	0.0	9.753799470661303E-5
4	0.0	0.0	0.0	0.0	9.753799470661303E-5
5	0.0	0.0	0.0	0.0	9.753799470661303E-5
6	0.0	0.0	0.0	0.0	1.9507598941322606E-4
7	0.0	0.0	0.0	9.753799470661303E-5	1.9507598941322606E-4
8	0.0	0.0	0.0	9.753799470661303E-5	1.9507598941322606E-4
9	0.0	0.0	0.0	1.4630699205991953E-4	1.9507598941322606E-4
10	0.0	0.0	0.0	1.4630699205991953E-4	1.9507598941322606E-4
11	0.0	0.0	0.0	1.4630699205991953E-4	1.9507598941322606E-4
12	0.0	0.0	0.0	1.4630699205991953E-4	2.9261398411983906E-4
13	0.0	0.0	0.0	1.4630699205991953E-4	3.413829814731456E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	125	0.0	14.44466	16
AACCGGT	60	2.5671481E-5	14.251117	14
AATCGTA	40	0.005277971	14.25077	13
GCGTTAT	195	0.0	14.118967	1
AAGACGG	495	0.0	13.432772	5
CAAGACG	505	0.0	13.354548	4
TCGCGTA	185	0.0	13.352073	9
CGTTATT	205	0.0	12.9674425	2
CGCCAGT	400	0.0	12.829765	18
GCGTAAC	200	0.0	12.825067	11
CGCGCCA	90	5.4058364E-7	12.667042	10
CGCAAGA	455	0.0	12.5195875	2
GTTATTC	250	0.0	12.155609	3
AGGCGTA	95	1.0396197E-6	12.0006485	12
GCAAGAC	595	0.0	11.970467	3
ACGGTAT	390	0.0	11.936542	9
ACGAACG	160	3.6379788E-12	11.875641	15
ATCGCCA	430	0.0	11.713081	16
TCCAACG	155	2.5465852E-11	11.649488	18
CTAGACA	155	2.5465852E-11	11.643516	4
>>END_MODULE