FastQCFastQC Report
Thu 2 Feb 2017
SRR4062660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050483
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32090.15649971250676062No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28740.14016209839340293No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24720.1205569614573737No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24430.11914266053412782No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24340.11870373955794805No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23100.11265638388613805No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21740.10602380024608835No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20510.10002521357163165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA755.4752054E-520.53324110
TGTAGGA12250.019.7554952
TTAACGG1452.9649527E-1019.7240535
GTAGGAC12250.019.3958343
TAGGACG8400.018.8570584
ATTTCGT5750.018.36513542
GATTTCG5800.017.82750741
CTGTAGG13300.017.702191
AGGACGT8800.017.4999245
TCGTATA902.2163938E-417.11103444
TACACCG902.2163938E-417.1110345
TTAGGAC5850.016.9230023
GTATTAC2109.094947E-1216.7646921
ATGCGTA1053.7765545E-516.7618322
TTAGTAC1053.7765545E-516.761833
CGAGCCG8450.016.66264715
GTCCTAG3600.016.5027431
AATCACG6850.016.37948834
GGACGTG9200.016.2607986
TATACCG953.3478483E-416.2104555
Produced by FastQC (version 0.11.5)