##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2050483
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26283660971586	32.0	32.0	32.0	32.0	32.0
2	31.422789654925204	32.0	32.0	32.0	32.0	32.0
3	31.515272255366174	32.0	32.0	32.0	32.0	32.0
4	31.616544004510157	32.0	32.0	32.0	32.0	32.0
5	31.556946826674494	32.0	32.0	32.0	32.0	32.0
6	35.16080796573295	36.0	36.0	36.0	36.0	36.0
7	35.17346059440629	36.0	36.0	36.0	36.0	36.0
8	35.118847120410166	36.0	36.0	36.0	36.0	36.0
9	35.24048772898873	36.0	36.0	36.0	36.0	36.0
10	35.12030872726085	36.0	36.0	36.0	36.0	36.0
11	35.24673357447977	36.0	36.0	36.0	36.0	36.0
12	35.14894295636687	36.0	36.0	36.0	36.0	36.0
13	35.18903058450131	36.0	36.0	36.0	36.0	36.0
14	35.152682563083914	36.0	36.0	36.0	36.0	36.0
15	35.12359722075238	36.0	36.0	36.0	36.0	36.0
16	35.13830985187393	36.0	36.0	36.0	36.0	36.0
17	35.10751759463502	36.0	36.0	36.0	36.0	36.0
18	35.1210870804586	36.0	36.0	36.0	36.0	36.0
19	35.098429004288256	36.0	36.0	36.0	36.0	36.0
20	35.08182706220924	36.0	36.0	36.0	36.0	36.0
21	35.06827123170492	36.0	36.0	36.0	36.0	36.0
22	35.053689301496284	36.0	36.0	36.0	36.0	36.0
23	35.01474725710967	36.0	36.0	36.0	36.0	36.0
24	34.989848245510935	36.0	36.0	36.0	36.0	36.0
25	34.95905501289209	36.0	36.0	36.0	36.0	36.0
26	34.91715366574607	36.0	36.0	36.0	32.0	36.0
27	34.899081338396854	36.0	36.0	36.0	32.0	36.0
28	34.87543471465016	36.0	36.0	36.0	32.0	36.0
29	34.846561517457104	36.0	36.0	36.0	32.0	36.0
30	34.82417703536191	36.0	36.0	36.0	32.0	36.0
31	34.81277923299047	36.0	36.0	36.0	32.0	36.0
32	34.775369510500695	36.0	36.0	36.0	32.0	36.0
33	34.74705423063737	36.0	36.0	36.0	32.0	36.0
34	34.72477704033635	36.0	36.0	36.0	32.0	36.0
35	34.68330290960715	36.0	36.0	36.0	32.0	36.0
36	34.6514206652774	36.0	36.0	36.0	32.0	36.0
37	34.625411671298906	36.0	36.0	36.0	32.0	36.0
38	34.57910209448213	36.0	36.0	36.0	32.0	36.0
39	34.53717733821738	36.0	36.0	36.0	32.0	36.0
40	34.505129766986606	36.0	36.0	36.0	32.0	36.0
41	34.47691446356785	36.0	36.0	36.0	32.0	36.0
42	34.41836728224521	36.0	36.0	36.0	32.0	36.0
43	34.41758356445774	36.0	36.0	36.0	32.0	36.0
44	34.37496872688045	36.0	36.0	36.0	32.0	36.0
45	34.30325342858244	36.0	36.0	36.0	32.0	36.0
46	34.31873319603235	36.0	36.0	36.0	32.0	36.0
47	34.26781787510552	36.0	36.0	36.0	32.0	36.0
48	34.23671398397353	36.0	36.0	36.0	32.0	36.0
49	34.22297234358929	36.0	36.0	36.0	32.0	36.0
50	33.69167849721261	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	20.0
21	56.0
22	187.0
23	552.0
24	1539.0
25	3587.0
26	7241.0
27	13713.0
28	22856.0
29	34559.0
30	47913.0
31	68362.0
32	100005.0
33	157588.0
34	345861.0
35	1246439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.909941307105825	17.638890176049415	11.3500731405259	26.101095376318856
2	15.99991026136864	20.500107540044645	36.9344210565846	26.56556114200212
3	18.93130895291862	24.35386796497	28.502670849770613	28.212152232340763
4	12.206080728493552	15.342227519632006	35.83466879430065	36.617022957573795
5	14.1092610862904	36.96650984182751	33.49230400837266	15.431925063509427
6	34.778189770242804	35.1500071447069	16.596837113850537	13.47496597119976
7	29.990007232442306	30.480184424840388	20.904928253489544	18.62488008922776
8	28.321487338099043	32.92962337635736	19.65191598853343	19.096973297010166
9	27.3984227130876	13.818158941088516	18.637803873526384	40.1456144722975
10	15.872894337578025	26.830605276903054	31.714283902865816	25.582216482653113
11	37.78382946847157	20.770520896783832	22.20091558915631	19.24473404558828
12	24.50003243138324	23.597171983381475	29.21145895869412	22.691336626541162
13	29.937190408308677	19.112911445742295	25.541738214849868	25.40815993109916
14	23.38473423091047	19.77792549365198	25.158706509636996	31.678633765800544
15	25.188796981003986	27.525514720190316	22.76726995542026	24.518418343385438
16	25.650883230926567	25.58782491734874	24.592839833346584	24.168452018378108
17	23.640625160023273	25.96685756477864	25.93545033048311	24.457066944714978
18	24.95373041376105	24.313393478512136	27.39491134527816	23.337964762448653
19	25.349344520291073	25.326618167524433	25.388164642184304	23.93587267000019
20	25.330470918315346	24.19429958697536	26.039669677827128	24.43555981688217
21	26.35832630653363	24.438047035747186	24.449068829149034	24.75455782857015
22	25.205037057122638	24.83956219095696	25.372802408018014	24.582598343902387
23	23.758646133618274	24.35904126003483	25.895459752653398	25.9868528536935
24	24.331047855554033	25.46346397409781	25.619671072620452	24.585817097727706
25	24.884429668522003	24.492570774788184	25.540031299942502	25.08296825674731
26	23.84087066315595	25.43971347238675	26.368860409961947	24.350555454495357
27	25.062972967832458	25.04751319567146	25.551101862341703	24.33841197415438
28	24.14382367471469	24.703789302325355	26.065029556450845	25.087357466509108
29	23.89002981248808	24.982309046210087	26.1580320344036	24.96962910689823
30	24.251213006886672	25.064240961763645	26.404266702040445	24.28027932930924
31	24.525538616999018	24.93631988170592	25.239321662262014	25.298819839033047
32	24.120268248993042	25.275166875316696	25.303209048794844	25.30135582689542
33	23.887298748636297	24.76660377091641	25.966516181797168	25.379581298650123
34	24.579282052082362	25.2362004464314	26.214360226346674	23.970157275139563
35	25.370168882160936	24.935490808750913	25.900336652388727	23.794003656699424
36	24.03687326351889	25.61518432486395	25.67224405176732	24.67569835984985
37	25.17884810554391	25.284920674787354	25.338907954857465	24.197323264811267
38	24.352067293413306	25.12890865225413	25.485605098896215	25.033418955436353
39	24.940611553473012	24.75265583767337	25.349588365277842	24.957144243575783
40	25.55446692315908	24.973628164681198	25.494042135438335	23.977862776721388
41	23.937384508918143	25.182847163326883	26.515606322998043	24.364162004756928
42	25.71652630136412	25.721451970096805	25.53203318437656	23.02998854416252
43	24.336705059247016	24.41493053100172	25.873903855823237	25.374460553928024
44	23.910220177392354	25.237175826378465	25.826841773377296	25.02576222285188
45	24.21112489106225	25.439274551410566	25.5702193093042	24.77938124822298
46	23.869277501230197	24.88011349532134	25.978148541732406	25.272460461716058
47	24.578062827148532	24.589035851553025	26.27746730892185	24.555434012376594
48	24.97645676652769	25.852250420998367	24.75665489545634	24.4146379170176
49	23.936799280949902	25.456733852463053	25.7855832016164	24.820883664970644
50	23.784249857228758	26.37958958937967	24.635220092046605	25.200940461344963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	45.5
2	58.0
3	204.0
4	350.0
5	414.0
6	478.0
7	433.0
8	388.0
9	564.5
10	741.0
11	1090.0
12	1439.0
13	2397.0
14	3355.0
15	4655.0
16	5955.0
17	6889.0
18	7823.0
19	8396.0
20	8969.0
21	10097.0
22	11225.0
23	12066.5
24	12908.0
25	13904.5
26	14901.0
27	18855.0
28	22809.0
29	25970.5
30	29132.0
31	32158.5
32	35185.0
33	41140.0
34	47095.0
35	54722.0
36	62349.0
37	71791.0
38	81233.0
39	84956.0
40	88679.0
41	95250.0
42	101821.0
43	104904.5
44	107988.0
45	121693.0
46	135398.0
47	146685.0
48	157972.0
49	159710.5
50	161449.0
51	151173.0
52	140897.0
53	137599.0
54	134301.0
55	136516.5
56	138732.0
57	135240.5
58	131749.0
59	120774.0
60	109799.0
61	97172.0
62	84545.0
63	73418.5
64	62292.0
65	53224.5
66	44157.0
67	38518.5
68	32880.0
69	30129.5
70	27379.0
71	21186.5
72	14994.0
73	13499.0
74	12004.0
75	8622.0
76	5240.0
77	4785.0
78	4330.0
79	3643.5
80	2957.0
81	2364.5
82	1772.0
83	1547.5
84	1323.0
85	1083.0
86	843.0
87	619.5
88	396.0
89	246.0
90	96.0
91	66.0
92	36.0
93	23.5
94	11.0
95	9.0
96	7.0
97	6.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015606079153058085
2	0.004096595777677747
3	5.852279682396782E-4
4	2.4384498676653258E-4
5	0.0
6	6.827659629462912E-4
7	0.0
8	4.876899735330651E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.753799470661301E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2050483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.69208040490825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.00066925096081	35.292458679338445
2	14.619874487352336	13.945044591209488
3	4.870128395813016	6.967996651060236
4	2.179678527221218	4.158136143083453
5	1.2109343584342633	2.8875989393756436
6	0.7036484088563315	2.0135073895177147
7	0.45088555931998414	1.50525692439505
8	0.3231763797440471	1.2330363110176208
9	0.23021015457080019	0.9881281081635264
>10	1.207508898395489	10.275429329297475
>50	0.09608194755156739	3.2057460955563983
>100	0.08839597992528234	9.085449746484345
>500	0.013155078892993239	4.299848278836105
>1k	0.005652572961833032	4.14236281266454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3209	0.15649971250676062	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2874	0.14016209839340293	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2472	0.1205569614573737	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2443	0.11914266053412782	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2434	0.11870373955794805	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2310	0.11265638388613805	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2174	0.10602380024608835	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2051	0.10002521357163165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.8768997353306514E-5
7	0.0	0.0	0.0	0.0	4.8768997353306514E-5
8	0.0	0.0	0.0	0.0	4.8768997353306514E-5
9	0.0	0.0	0.0	0.0	4.8768997353306514E-5
10	0.0	0.0	0.0	4.8768997353306514E-5	4.8768997353306514E-5
11	0.0	0.0	0.0	4.8768997353306514E-5	4.8768997353306514E-5
12	0.0	0.0	0.0	4.8768997353306514E-5	1.9507598941322606E-4
13	0.0	0.0	0.0	4.8768997353306514E-5	3.413829814731456E-4
14	0.0	0.0	0.0	4.8768997353306514E-5	3.413829814731456E-4
15	0.0	0.0	0.0	9.753799470661303E-5	3.413829814731456E-4
16	0.0	0.0	0.0	2.4384498676653256E-4	3.413829814731456E-4
17	0.0	0.0	0.0	2.9261398411983906E-4	3.413829814731456E-4
18	0.0	0.0	0.0	3.901519788264521E-4	3.413829814731456E-4
19	0.0	0.0	0.0	5.852279682396781E-4	3.413829814731456E-4
20	0.0	0.0	0.0	9.266109497128238E-4	3.901519788264521E-4
21	0.0	0.0	0.0	0.0013167629285392758	4.3892097617975864E-4
22	0.0	0.0	0.0	0.0024384498676653255	5.364589708863717E-4
23	0.0	0.0	0.0	0.004828130737977345	5.364589708863717E-4
24	0.0	0.0	0.0	0.0073641186003492835	5.364589708863717E-4
25	0.0	0.0	0.0	0.008875957518301785	5.852279682396781E-4
26	0.0	0.0	0.0	0.01175332836214687	6.827659629462912E-4
27	0.0	0.0	0.0	0.015411003163644858	6.827659629462912E-4
28	0.0	0.0	0.0	0.02350665672429374	7.315349602995977E-4
29	0.0	0.0	0.0	0.03584521305468029	7.315349602995977E-4
30	0.0	0.0	0.0	0.06378984853812492	7.315349602995977E-4
31	0.0	0.0	0.0	0.11802097359500176	7.315349602995977E-4
32	0.0	0.0	0.0	0.17937237226546135	7.315349602995977E-4
33	0.0	0.0	0.0	0.23643209916882998	7.315349602995977E-4
34	0.0	0.0	0.0	0.3028554735640335	7.803039576529042E-4
35	0.0	0.0	0.0	0.3785449574563652	7.803039576529042E-4
36	0.0	0.0	0.0	0.4906648823716168	7.803039576529042E-4
37	0.0	0.0	0.0	0.6483350508148568	7.803039576529042E-4
38	0.0	0.0	0.0	0.8516530007807916	7.803039576529042E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGA	75	5.4752054E-5	20.533241	10
TGTAGGA	1225	0.0	19.755495	2
TTAACGG	145	2.9649527E-10	19.72405	35
GTAGGAC	1225	0.0	19.395834	3
TAGGACG	840	0.0	18.857058	4
ATTTCGT	575	0.0	18.365135	42
GATTTCG	580	0.0	17.827507	41
CTGTAGG	1330	0.0	17.70219	1
AGGACGT	880	0.0	17.499924	5
TCGTATA	90	2.2163938E-4	17.111034	44
TACACCG	90	2.2163938E-4	17.111034	5
TTAGGAC	585	0.0	16.923002	3
GTATTAC	210	9.094947E-12	16.764692	1
ATGCGTA	105	3.7765545E-5	16.76183	22
TTAGTAC	105	3.7765545E-5	16.76183	3
CGAGCCG	845	0.0	16.662647	15
GTCCTAG	360	0.0	16.502743	1
AATCACG	685	0.0	16.379488	34
GGACGTG	920	0.0	16.260798	6
TATACCG	95	3.3478483E-4	16.210455	5
>>END_MODULE