##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062659_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42401
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.53972783660763	32.0	32.0	32.0	14.0	32.0
2	28.177401476380275	32.0	32.0	32.0	14.0	32.0
3	28.1357043465956	32.0	32.0	32.0	14.0	32.0
4	28.179618405226293	32.0	32.0	32.0	14.0	32.0
5	27.281290535600576	32.0	21.0	32.0	14.0	32.0
6	31.092285559302848	36.0	32.0	36.0	14.0	36.0
7	30.73875616141129	36.0	32.0	36.0	14.0	36.0
8	30.645692318577392	36.0	32.0	36.0	14.0	36.0
9	31.27987547463503	36.0	32.0	36.0	14.0	36.0
10	29.92504893752506	36.0	21.0	36.0	14.0	36.0
11	31.444753661470248	36.0	32.0	36.0	14.0	36.0
12	30.434423716421783	36.0	27.0	36.0	14.0	36.0
13	30.92129902596637	36.0	32.0	36.0	14.0	36.0
14	30.605221574962854	36.0	32.0	36.0	14.0	36.0
15	30.35798683993302	36.0	27.0	36.0	14.0	36.0
16	30.38968420556119	36.0	27.0	36.0	14.0	36.0
17	30.167637555718027	36.0	27.0	36.0	14.0	36.0
18	30.258484469705902	36.0	27.0	36.0	14.0	36.0
19	30.245182896629796	36.0	27.0	36.0	14.0	36.0
20	30.299214641164124	36.0	27.0	36.0	14.0	36.0
21	30.291172378009954	36.0	27.0	36.0	14.0	36.0
22	30.211433692601588	36.0	27.0	36.0	14.0	36.0
23	29.96174618523148	36.0	27.0	36.0	14.0	36.0
24	29.99591990754935	36.0	27.0	36.0	14.0	36.0
25	29.43836230277588	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	4.0
6	8.0
7	2.0
8	8.0
9	7.0
10	4.0
11	3.0
12	3.0
13	2.0
14	23.0
15	61.0
16	168.0
17	357.0
18	591.0
19	700.0
20	882.0
21	1059.0
22	1129.0
23	1188.0
24	1243.0
25	1315.0
26	1650.0
27	1749.0
28	1969.0
29	2372.0
30	2768.0
31	3490.0
32	4193.0
33	4850.0
34	6628.0
35	3974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.105901221755744	18.439549035331858	10.755224303033161	25.69932543987924
2	20.69811320754717	19.15566037735849	36.00471698113207	24.141509433962263
3	21.854289016250384	22.51704049623812	29.123328380386333	26.50534210712517
4	14.996344080949124	13.812298039955657	36.490789442649245	34.70056843644598
5	18.299127152630337	34.142486435480066	33.08563340410474	14.472753007784856
6	36.23516644254134	34.75358010710831	16.497982872107013	12.51327057824333
7	32.530973451327434	28.984070796460177	21.267256637168142	17.217699115044248
8	30.76995704102346	31.54652315536043	19.411792475097954	18.27172732851815
9	29.766541557491205	13.214361589122584	19.040200174680734	37.978896678705475
10	18.697612919982056	24.371355039784667	31.768705876797394	25.162326163435882
11	39.675606761733874	20.34186419869676	22.103125885352725	17.87940315421664
12	26.89020070838253	22.861865407319954	28.649350649350648	21.598583234946872
13	31.47356739782306	18.97150142847024	24.860104360966165	24.69482681274054
14	25.877824742024607	18.992183994899527	25.195400127511864	29.934591135564006
15	27.79063178770422	25.916044952308997	22.029936726791956	24.263386533194826
16	28.386259001298548	24.47881005784441	24.098689647031048	23.036241293825995
17	25.99116862263572	25.4079199036577	25.474037167347518	23.126874306359063
18	27.708254627880617	23.33537967510389	26.336418587079713	22.619947109935776
19	28.095114406479492	24.161137216935465	25.185954804127608	22.557793572457438
20	28.246960217211665	23.140125132806045	24.925038366190535	23.68787628379176
21	29.025783906309027	23.62344163203627	23.87608613524745	23.474688326407254
22	28.28263744568298	23.897128282637446	24.718968448894767	23.10126582278481
23	26.80882214036082	23.422593747048268	25.517143666761122	24.251440445829793
24	27.062573789846518	24.460448642266826	25.322314049586776	23.15466351829988
25	28.146031596098897	23.08782204170307	25.15644555694618	23.609700805251848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	5.0
2	10.0
3	15.0
4	15.0
5	15.0
6	47.5
7	80.0
8	80.0
9	80.0
10	93.5
11	107.0
12	107.0
13	107.0
14	159.0
15	211.0
16	211.0
17	211.0
18	315.0
19	419.0
20	419.0
21	419.0
22	642.5
23	866.0
24	866.0
25	866.0
26	1129.0
27	1392.0
28	1392.0
29	1392.0
30	1737.0
31	2082.0
32	2082.0
33	2082.0
34	2525.5
35	2969.0
36	2969.0
37	2969.0
38	3233.0
39	3497.0
40	3497.0
41	3497.0
42	3780.0
43	4063.0
44	4063.0
45	4063.0
46	4494.0
47	4925.0
48	4925.0
49	4925.0
50	4912.5
51	4900.0
52	4900.0
53	4900.0
54	4592.0
55	4284.0
56	4284.0
57	4284.0
58	3802.0
59	3320.0
60	3320.0
61	3320.0
62	2999.0
63	2678.0
64	2678.0
65	2678.0
66	2327.5
67	1977.0
68	1977.0
69	1977.0
70	1606.0
71	1235.0
72	1235.0
73	1235.0
74	1030.0
75	825.0
76	825.0
77	825.0
78	732.5
79	640.0
80	640.0
81	640.0
82	554.0
83	468.0
84	468.0
85	468.0
86	442.5
87	417.0
88	417.0
89	417.0
90	378.0
91	339.0
92	339.0
93	339.0
94	321.0
95	303.0
96	303.0
97	303.0
98	343.5
99	384.0
100	384.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007075304827716328
2	0.0023584349425721093
3	0.004716869885144219
4	0.009433739770288437
5	0.0259427843682932
6	0.03301808919600953
7	0.061319308506874844
8	0.08254522299002383
9	0.08962052781774014
10	0.11320487724346125
11	0.10612957241574492
12	0.12028018207117758
13	0.11320487724346125
14	0.12263861701374969
15	0.10612957241574492
16	0.10848800735831703
17	0.12263861701374969
18	0.11556331218603336
19	0.12263861701374969
20	0.10848800735831703
21	0.11556331218603336
22	0.1344307917266102
23	0.1249970519563218
24	0.12028018207117758
25	0.1273554868988939
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	42401.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.43671139831608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.32698287071061	78.70333246857385
2	2.9001074113856067	4.839508502157968
3	0.8423313923907513	2.1084408386594653
4	0.49183108146305615	1.641470720030188
5	0.3589801571598168	1.4976061885332892
6	0.20068969416021254	1.0046932855357185
7	0.1780767708745548	1.0400698096743
8	0.14133077053536097	0.9433739770288437
9	0.0734920006783877	0.5518737765618735
>10	0.4776980044095201	6.91021438173628
>50	0.005653230821414439	0.3183887172472347
>100	0.0028266154107072193	0.44102733426098445
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	187	0.44102733426098445	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	81	0.19103323034834083	No Hit
GTATCAACGCAGAGTACTTTTTTTT	54	0.1273554868988939	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	48	0.11320487724346125	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	47	0.11084644230088914	No Hit
GAACTACGACGGTATCTGATCGTCT	44	0.1037711374731728	No Hit
ATCAGATACCGTCGTAGTTCCGACC	43	0.1014127025306007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE