FastQCFastQC Report
Thu 2 Feb 2017
SRR4062659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42401
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG2960.6980967430013444No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC880.2075422749463456No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG630.14858140138204287No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC490.11556331218603336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAC404.000849E-738.543
CGGAACT451.0062322E-634.2222241
CGGCGCA451.0062322E-634.2222233
GGTCGGA451.0062322E-634.2222238
TCGGAAC451.0062322E-634.2222240
GGCGCAA451.0062322E-634.2222234
TGGTCGG451.0062322E-634.2222237
GTCGGAA451.0062322E-634.2222239
TGGACCG353.1661885E-431.42857228
TATGGTC502.2913227E-630.80000135
ATCGTTT502.2913227E-630.80000129
TGCGCCG502.2913227E-630.8000015
TTGCGCC502.2913227E-630.8000014
CGTTTAT502.2913227E-630.80000131
GAAATTC453.9787803E-529.33333420
GTATTGC300.005683512429.3333321
TATTGCG300.005683512429.3333322
ATTGCGC300.005683512429.3333323
TTATGGT554.8145776E-628.034
GGAACTA554.8145776E-628.042
Produced by FastQC (version 0.11.5)