##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062659_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42401
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.310110610598805	32.0	32.0	32.0	21.0	32.0
2	28.329119596235937	32.0	32.0	32.0	14.0	32.0
3	30.174712860545743	32.0	32.0	32.0	21.0	32.0
4	30.88903563595198	32.0	32.0	32.0	32.0	32.0
5	30.42588618192967	32.0	32.0	32.0	21.0	32.0
6	34.048418669371	36.0	36.0	36.0	32.0	36.0
7	34.105917313270915	36.0	36.0	36.0	32.0	36.0
8	33.93092144053206	36.0	36.0	36.0	32.0	36.0
9	34.44777245819674	36.0	36.0	36.0	32.0	36.0
10	33.752553005825334	36.0	36.0	36.0	32.0	36.0
11	34.62236739699535	36.0	36.0	36.0	32.0	36.0
12	34.178392019056155	36.0	36.0	36.0	32.0	36.0
13	34.42951817174123	36.0	36.0	36.0	32.0	36.0
14	34.28383764533855	36.0	36.0	36.0	32.0	36.0
15	34.200891488408296	36.0	36.0	36.0	32.0	36.0
16	34.24919223603217	36.0	36.0	36.0	32.0	36.0
17	34.17591566236646	36.0	36.0	36.0	32.0	36.0
18	34.247022475885004	36.0	36.0	36.0	32.0	36.0
19	34.26886158345322	36.0	36.0	36.0	32.0	36.0
20	34.24900356123676	36.0	36.0	36.0	32.0	36.0
21	34.240324520648095	36.0	36.0	36.0	32.0	36.0
22	34.23480578288248	36.0	36.0	36.0	32.0	36.0
23	34.162802764085754	36.0	36.0	36.0	32.0	36.0
24	34.12829886087592	36.0	36.0	36.0	32.0	36.0
25	34.080540553288834	36.0	36.0	36.0	32.0	36.0
26	33.95153416193014	36.0	36.0	36.0	32.0	36.0
27	33.93934105327705	36.0	36.0	36.0	32.0	36.0
28	33.88290370510129	36.0	36.0	36.0	32.0	36.0
29	33.78858989174783	36.0	36.0	36.0	32.0	36.0
30	33.83019268413481	36.0	36.0	36.0	32.0	36.0
31	33.82335322280135	36.0	36.0	36.0	32.0	36.0
32	33.73847314921818	36.0	36.0	36.0	32.0	36.0
33	33.731114832197356	36.0	36.0	36.0	32.0	36.0
34	33.683380108959696	36.0	36.0	36.0	32.0	36.0
35	33.78231645480059	36.0	36.0	36.0	32.0	36.0
36	33.64274427489918	36.0	36.0	36.0	27.0	36.0
37	33.62630598334945	36.0	36.0	36.0	27.0	36.0
38	33.60840546213533	36.0	36.0	36.0	27.0	36.0
39	33.58878328341313	36.0	36.0	36.0	27.0	36.0
40	33.59255677932124	36.0	36.0	36.0	27.0	36.0
41	33.48553100162732	36.0	36.0	36.0	21.0	36.0
42	33.491993113369965	36.0	36.0	36.0	21.0	36.0
43	33.51501143840947	36.0	36.0	36.0	21.0	36.0
44	33.417773165727226	36.0	36.0	36.0	21.0	36.0
45	33.35223225867315	36.0	36.0	36.0	21.0	36.0
46	33.32084148958751	36.0	36.0	36.0	21.0	36.0
47	33.32751586047499	36.0	36.0	36.0	21.0	36.0
48	33.30928515836891	36.0	36.0	36.0	21.0	36.0
49	33.23027758779274	36.0	36.0	36.0	21.0	36.0
50	32.48612061036296	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	3.0
22	8.0
23	22.0
24	63.0
25	118.0
26	245.0
27	430.0
28	776.0
29	1353.0
30	2064.0
31	3342.0
32	4901.0
33	7556.0
34	11080.0
35	10436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.14150943396226	18.193396226415096	11.481132075471697	22.183962264150946
2	20.867924528301888	21.599056603773583	35.6061320754717	21.92688679245283
3	22.785377358490567	23.860849056603772	30.125	23.22877358490566
4	17.45477700997618	17.6222258908988	37.635904813565716	27.2870922855593
5	21.454682672578475	31.75396806679088	31.685573453456286	15.10577580717436
6	33.31053512888847	37.994386924836675	15.471333223273037	13.223744723001815
7	29.02997570812009	30.890780877809487	20.3957453833636	19.683498030706822
8	28.24697530718615	33.15723685762128	18.433527511143605	20.16226032404896
9	26.037121765996083	13.74495884531025	18.09391287941322	42.12400650928044
10	14.877007617744864	26.0441970708238	31.558217966557393	27.520577344873942
11	38.24438102874932	20.754227494634563	21.987688969599773	19.013702507016344
12	23.49237046296078	23.798967005495154	28.558288719605667	24.150373811938397
13	28.794132213862884	19.584443763118795	25.001768826206927	26.619655196811397
14	23.541897596754794	19.90283248036603	24.71168132827056	31.84358859460862
15	25.284781019315584	26.617296761868825	21.836749133275156	26.261173085540435
16	25.36025093747789	26.805971557274592	23.082002782953232	24.751774722294286
17	23.24001792410556	26.112591684158392	25.704582439093414	24.942807952642625
18	24.8013018560883	25.178651446899835	25.50175703403222	24.518289662979647
19	25.409778071271905	26.070139855192092	25.308365368741303	23.2117167047947
20	24.84611211999717	25.23761232046414	24.78007594160515	25.136199617933542
21	25.864956015188323	24.947524822527768	24.268295559067003	24.919223603216906
22	25.032428480460368	25.824862621164595	24.999410391264355	24.143298507110682
23	24.537157142520226	25.041862220230655	25.942784368293196	24.47819626895592
24	24.25650338435414	25.136199617933542	26.2894743048513	24.317822692861018
25	24.14094007216811	25.178651446899835	25.923916888752625	24.75649159217943
26	24.843753685054597	25.308365368741303	25.544208862998513	24.303672083205583
27	25.23761232046414	24.928657342987194	25.23997075540671	24.593759581141956
28	23.600858470319096	25.645621565529115	25.881465059786322	24.872054904365463
29	24.270653994009574	25.810712011509164	25.31308223862645	24.605551755854815
30	23.888587533312894	25.48288955449164	26.76823659819344	23.86028631400203
31	24.348482347114455	25.82722105610717	25.129124313105823	24.695172283672555
32	24.912148298389187	25.461663640008492	24.862621164595176	24.76356689700715
33	23.66217777882597	25.143274922761254	26.633805806466825	24.560741491945944
34	24.232919034928422	25.591377561849953	26.209287516803847	23.966415886417774
35	24.93809108275748	25.11025683356525	26.374377962783896	23.577274120893374
36	23.803683875380298	26.180986297492986	25.176293011957263	24.839036815169454
37	24.940449517700053	25.25176293011957	26.011178981627793	23.796608570552582
38	24.239994339756137	25.15978396735926	25.98759463220207	24.61262706068253
39	24.638569845050824	25.622037216103394	24.75649159217943	24.982901346666353
40	24.511214358151932	26.025329591283224	26.0441970708238	23.419258979741045
41	23.308412537440155	25.669205914954834	26.45456475083135	24.567816796773663
42	25.1479917926464	26.2894743048513	25.400344331501618	23.162189571000685
43	24.423952265276764	25.206952666210704	26.096082639560386	24.27301242895215
44	23.869720053772316	26.53239310393623	25.289497889200725	24.308388953090727
45	24.190467205962126	26.919176434518054	25.544208862998513	23.346147496521308
46	24.150373811938397	25.78476922714087	24.758850027122	25.30600693379873
47	24.32253956274616	25.006485696092074	26.492299709912505	24.178675031249263
48	24.643286714935968	26.487582840027358	25.15270866253154	23.71642178250513
49	23.83906039951888	25.817787316336876	25.692790264380555	24.650362019763687
50	24.638569845050824	25.930992193580337	25.296573194028444	24.133864767340395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	2.5
4	3.0
5	2.5
6	2.0
7	3.5
8	5.0
9	6.5
10	8.0
11	12.5
12	17.0
13	17.0
14	17.0
15	36.5
16	56.0
17	96.0
18	136.0
19	139.5
20	143.0
21	192.0
22	241.0
23	286.0
24	331.0
25	381.5
26	432.0
27	582.5
28	733.0
29	840.5
30	948.0
31	968.5
32	989.0
33	1075.5
34	1162.0
35	1299.0
36	1436.0
37	1650.0
38	1864.0
39	1969.0
40	2074.0
41	2202.5
42	2331.0
43	2313.0
44	2295.0
45	2390.0
46	2485.0
47	2561.0
48	2637.0
49	2719.5
50	2802.0
51	2647.0
52	2492.0
53	2452.5
54	2413.0
55	2428.0
56	2443.0
57	2649.0
58	2855.0
59	2453.5
60	2052.0
61	1840.5
62	1629.0
63	1481.5
64	1334.0
65	1157.0
66	980.0
67	889.0
68	798.0
69	781.0
70	764.0
71	603.5
72	443.0
73	390.5
74	338.0
75	264.5
76	191.0
77	166.0
78	141.0
79	133.5
80	126.0
81	107.5
82	89.0
83	74.0
84	59.0
85	52.5
86	46.0
87	35.5
88	25.0
89	18.5
90	12.0
91	12.5
92	13.0
93	7.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0023584349425721093
2	0.0023584349425721093
3	0.0023584349425721093
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	42401.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.54993985990896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.48322952974117	77.17034975590198
2	3.5912233586652196	5.929105445626282
3	0.9456602479858294	2.341925897974104
4	0.5799668590366265	1.9150491733685528
5	0.27427004171190217	1.1320487724346124
6	0.23712930689674874	1.1745006014009103
7	0.17141877606993886	0.9905426758802859
8	0.09999428604079766	0.6603617839201906
9	0.06571053082680989	0.48819603311242665
>10	0.542826124221473	7.143699441050918
>50	0.005713959202331295	0.3561236763283885
>100	0.0028569796011656476	0.6980967430013444
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	296	0.6980967430013444	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	88	0.2075422749463456	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	63	0.14858140138204287	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	49	0.11556331218603336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.009433739770288437	0.0
31	0.0	0.0	0.0	0.014150609655432654	0.0
32	0.0	0.0	0.0	0.018867479540576874	0.0
33	0.0	0.0	0.0	0.0259427843682932	0.0
34	0.0	0.0	0.0	0.03537652413858164	0.0
35	0.0	0.0	0.0	0.05424400367915851	0.0
36	0.0	0.0	0.0	0.08962052781774014	0.0
37	0.0	0.0	0.0	0.11320487724346123	0.0
38	0.0	0.0	0.0	0.15565670620975922	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTAC	40	4.000849E-7	38.5	43
CGGAACT	45	1.0062322E-6	34.22222	41
CGGCGCA	45	1.0062322E-6	34.22222	33
GGTCGGA	45	1.0062322E-6	34.22222	38
TCGGAAC	45	1.0062322E-6	34.22222	40
GGCGCAA	45	1.0062322E-6	34.22222	34
TGGTCGG	45	1.0062322E-6	34.22222	37
GTCGGAA	45	1.0062322E-6	34.22222	39
TGGACCG	35	3.1661885E-4	31.428572	28
TATGGTC	50	2.2913227E-6	30.800001	35
ATCGTTT	50	2.2913227E-6	30.800001	29
TGCGCCG	50	2.2913227E-6	30.800001	5
TTGCGCC	50	2.2913227E-6	30.800001	4
CGTTTAT	50	2.2913227E-6	30.800001	31
GAAATTC	45	3.9787803E-5	29.333334	20
GTATTGC	30	0.0056835124	29.333332	1
TATTGCG	30	0.0056835124	29.333332	2
ATTGCGC	30	0.0056835124	29.333332	3
TTATGGT	55	4.8145776E-6	28.0	34
GGAACTA	55	4.8145776E-6	28.0	42
>>END_MODULE