FastQCFastQC Report
Thu 2 Feb 2017
SRR4062658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826621
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG22840.27630558623601387No Hit
GTATCAACGCAGAGTACTTTTTTTT22240.26904712074820264No Hit
TCCATGTACTCTGCGTTGATACCAC19740.2388035145489892No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16760.2027531359595268No Hit
TATCAACGCAGAGTACTTTTTTTTT15720.19017179578065402No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12920.15629895683753497No Hit
GAGTACATGGAAGCAGTGGTATCAA10510.12714412046149323No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10130.12254709231921279No Hit
CATGTACTCTGCGTTGATACCACTG8400.1016185168293571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG555.8942896E-717.27470815
ATAACGA601.4621037E-615.83610912
CGAACGA551.1251594E-515.54818116
GATAACG551.1251594E-515.54818111
TATACTG551.12904145E-515.5425315
TAACGAA602.5611467E-514.25249913
CCGATAA602.5626101E-514.2516359
CGATAAC602.5626101E-514.25163510
TCGAACG707.284996E-613.5655913
GCGTTAT707.294113E-613.5639491
TCTAGCC500.001503375813.294283
ATTCGAA655.4747015E-513.1465961
TTTTCGG751.4739371E-512.66888716
AACGTCT751.4748555E-512.668126
CGACCTG604.0844607E-412.66811910
AAGACGG1352.7284841E-1112.6642855
TTTATAC604.1032842E-412.6612193
CAAGACG1301.9826984E-1012.420744
CCGTCAA701.0879243E-412.21642718
TAGTACT701.0916217E-412.2119894
Produced by FastQC (version 0.11.5)