##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062658_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	826621
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04881439014978	32.0	32.0	32.0	32.0	32.0
2	30.656038256952097	32.0	32.0	32.0	32.0	32.0
3	30.668647421248675	32.0	32.0	32.0	32.0	32.0
4	30.73249530316796	32.0	32.0	32.0	32.0	32.0
5	30.50354757500717	32.0	32.0	32.0	32.0	32.0
6	34.12929504573438	36.0	36.0	36.0	32.0	36.0
7	33.999721758822965	36.0	36.0	36.0	32.0	36.0
8	33.96986164155036	36.0	36.0	36.0	32.0	36.0
9	34.15902209113971	36.0	36.0	36.0	32.0	36.0
10	33.78947788648002	36.0	36.0	36.0	32.0	36.0
11	34.18988266690539	36.0	36.0	36.0	32.0	36.0
12	33.91291535056574	36.0	36.0	36.0	32.0	36.0
13	34.06654198235951	36.0	36.0	36.0	32.0	36.0
14	33.961557957032305	36.0	36.0	36.0	32.0	36.0
15	33.899273064681395	36.0	36.0	36.0	32.0	36.0
16	33.89451030157714	36.0	36.0	36.0	32.0	36.0
17	33.819231546258806	36.0	36.0	36.0	32.0	36.0
18	33.82592264169432	36.0	36.0	36.0	32.0	36.0
19	33.81487162798913	36.0	36.0	36.0	32.0	36.0
20	33.800357116502	36.0	36.0	36.0	32.0	36.0
21	33.76531687435959	36.0	36.0	36.0	32.0	36.0
22	33.72735993883533	36.0	36.0	36.0	32.0	36.0
23	33.68842673970296	36.0	36.0	36.0	27.0	36.0
24	33.67671883487112	36.0	36.0	36.0	27.0	36.0
25	33.18706880178461	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	21.0
5	82.0
6	156.0
7	34.0
8	126.0
9	147.0
10	92.0
11	23.0
12	66.0
13	50.0
14	193.0
15	227.0
16	343.0
17	491.0
18	676.0
19	985.0
20	1463.0
21	2160.0
22	3077.0
23	4417.0
24	6011.0
25	8318.0
26	11182.0
27	14134.0
28	19296.0
29	25125.0
30	32320.0
31	43950.0
32	62762.0
33	89341.0
34	190519.0
35	308825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75949864801922	17.66309969572871	10.882870675627446	25.694530980624634
2	16.72667136058224	20.485070203072336	36.76606153868488	26.022196897660542
3	19.164969918253625	24.543390181966437	28.2849472707454	28.00669262903454
4	12.471611979774783	15.33527809672919	36.16001587430262	36.0330940491934
5	14.240661429862852	36.98239567437262	33.68468567680506	15.092257218959473
6	33.72866503837933	35.38378119303919	17.216838976260732	13.670714792320746
7	29.735501232971735	30.791123571974193	21.147553486277516	18.325821708776555
8	28.205379683612126	32.564824400491595	19.935947498743772	19.29384841715251
9	27.450721587565297	14.275058184733197	19.013632543732395	39.260587683969106
10	15.685673695646118	26.843063328638195	32.11546403738993	25.355798938325762
11	37.05423108040676	21.55423834739091	22.221953074660995	19.169577497541336
12	24.263034977978265	24.289805074932044	28.80692582381642	22.640234123273267
13	29.30561056585308	19.85724373579069	25.611704414833763	25.225441283522464
14	23.276846696373525	19.98866310652683	25.484391786566025	31.25009841053362
15	24.90989332532385	27.182906860702833	23.154750947088974	24.752448866884343
16	25.32608932606995	26.022352106228215	24.265284718067466	24.386273849634367
17	23.525550106405785	26.067903485046866	25.74583975785265	24.660706650694696
18	24.40021803646054	24.87117679123009	27.01362727878384	23.71497789352553
19	24.80582782997156	25.83228757346564	25.8448851440726	23.5169994524902
20	24.867068630134664	24.73068528392203	26.043768637644227	24.358477448299084
21	26.06363649577743	24.739693106773068	24.86481968690495	24.33185071054455
22	24.96671226188212	25.101074778980326	25.440070561523857	24.492142397613705
23	23.851449490428188	24.826983017503395	25.912268609092457	25.40929888297595
24	24.05567924953423	25.843050511560072	25.672490109192577	24.428780129713125
25	24.282393583968158	25.182513139613615	25.734988619804568	24.800104656613655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	208.0
1	208.0
2	467.0
3	726.0
4	726.0
5	726.0
6	1831.0
7	2936.0
8	2936.0
9	2936.0
10	3030.5
11	3125.0
12	3125.0
13	3125.0
14	3250.0
15	3375.0
16	3375.0
17	3375.0
18	5098.5
19	6822.0
20	6822.0
21	6822.0
22	10692.0
23	14562.0
24	14562.0
25	14562.0
26	20736.5
27	26911.0
28	26911.0
29	26911.0
30	32517.5
31	38124.0
32	38124.0
33	38124.0
34	45771.0
35	53418.0
36	53418.0
37	53418.0
38	59870.0
39	66322.0
40	66322.0
41	66322.0
42	75661.5
43	85001.0
44	85001.0
45	85001.0
46	98891.5
47	112782.0
48	112782.0
49	112782.0
50	110730.5
51	108679.0
52	108679.0
53	108679.0
54	99641.0
55	90603.0
56	90603.0
57	90603.0
58	82704.5
59	74806.0
60	74806.0
61	74806.0
62	66570.0
63	58334.0
64	58334.0
65	58334.0
66	48111.0
67	37888.0
68	37888.0
69	37888.0
70	28606.5
71	19325.0
72	19325.0
73	19325.0
74	15071.5
75	10818.0
76	10818.0
77	10818.0
78	8855.0
79	6892.0
80	6892.0
81	6892.0
82	4815.5
83	2739.0
84	2739.0
85	2739.0
86	2028.5
87	1318.0
88	1318.0
89	1318.0
90	874.0
91	430.0
92	430.0
93	430.0
94	256.0
95	82.0
96	82.0
97	82.0
98	238.5
99	395.0
100	395.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0067745677886238065
2	0.00229851407114022
3	0.004838976991874148
4	0.015484726373997273
5	0.03508258319108757
6	0.04778489779475721
7	0.0687134732846129
8	0.08903717665048433
9	0.09714246311187352
10	0.11419985700822988
11	0.11722421762815124
12	0.12980555780702402
13	0.12315196444319707
14	0.12073247594725998
15	0.1128691383354645
16	0.11214329178668339
17	0.12194222019522853
18	0.1295636089574303
19	0.1288377624086492
20	0.12182124577043167
21	0.12786996701027437
22	0.1502502355976923
23	0.13597525347166356
24	0.13391868825011705
25	0.12871678798385233
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	826621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.3730606309136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81941420878512	47.760496263952476
2	11.953731433584851	13.955517794766125
3	3.0152981940651915	5.280365529073551
4	1.1275481325264447	2.6327374201695823
5	0.518189878218701	1.5124164589792983
6	0.3010455559550665	1.0543770294259314
7	0.20134876765584603	0.8227340675634104
8	0.12901049486130353	0.602458995085043
9	0.1003783278997561	0.527345119846997
>10	0.6298833770004372	7.303399519809852
>50	0.1035349059556279	4.241361825871307
>100	0.09311734037775986	10.546171665043218
>500	0.005832853533105152	2.169353639583954
>1k	0.0016665295808871864	1.591264670829351
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2284	0.27630558623601387	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2224	0.26904712074820264	No Hit
TCCATGTACTCTGCGTTGATACCAC	1974	0.2388035145489892	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1676	0.2027531359595268	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1572	0.19017179578065402	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1292	0.15629895683753497	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1051	0.12714412046149323	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1013	0.12254709231921279	No Hit
CATGTACTCTGCGTTGATACCACTG	840	0.1016185168293571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.419488495937074E-4	0.0
12	0.0	0.0	0.0	2.419488495937074E-4	1.209744247968537E-4
13	0.0	0.0	0.0	2.419488495937074E-4	1.209744247968537E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAACG	55	5.8942896E-7	17.274708	15
ATAACGA	60	1.4621037E-6	15.836109	12
CGAACGA	55	1.1251594E-5	15.548181	16
GATAACG	55	1.1251594E-5	15.548181	11
TATACTG	55	1.12904145E-5	15.542531	5
TAACGAA	60	2.5611467E-5	14.252499	13
CCGATAA	60	2.5626101E-5	14.251635	9
CGATAAC	60	2.5626101E-5	14.251635	10
TCGAACG	70	7.284996E-6	13.565591	3
GCGTTAT	70	7.294113E-6	13.563949	1
TCTAGCC	50	0.0015033758	13.29428	3
ATTCGAA	65	5.4747015E-5	13.146596	1
TTTTCGG	75	1.4739371E-5	12.668887	16
AACGTCT	75	1.4748555E-5	12.66812	6
CGACCTG	60	4.0844607E-4	12.668119	10
AAGACGG	135	2.7284841E-11	12.664285	5
TTTATAC	60	4.1032842E-4	12.661219	3
CAAGACG	130	1.9826984E-10	12.42074	4
CCGTCAA	70	1.0879243E-4	12.216427	18
TAGTACT	70	1.0916217E-4	12.211989	4
>>END_MODULE