FastQCFastQC Report
Thu 2 Feb 2017
SRR4062658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826621
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9360.11323206160985506No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9210.11141744523790226No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8520.10307020992691934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA250.002352411635.1998522
CGTCTTA2300.022.95642915
TCTCGTC2350.022.46799312
TAGGACG1407.2759576E-1221.999914
CCACGCG500.002579816221.9999089
GTACGAA500.002579816221.99990819
CTAGCGG2550.020.70579729
TTCTCGT2600.020.3076111
CGGTCCA2850.020.07009310
CGCAATA2850.020.07009336
ACGCATT550.004482539419.9999220
TCTAGCG2650.019.92444628
GTTAGGC1001.1226002E-619.79991716
CAATACG2800.019.64277638
GTCTTAG909.99799E-619.5590231
TTACCCC901.0013671E-519.5554754
ATAGGGT3200.019.249923
CTCGTCT2900.018.96543913
GTCGATA1051.7913608E-618.85706536
TCGATAT951.596171E-518.5262437
Produced by FastQC (version 0.11.5)