##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062658_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	826621
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.213626317260267	32.0	32.0	32.0	32.0	32.0
2	31.345858622028718	32.0	32.0	32.0	32.0	32.0
3	31.449723633926553	32.0	32.0	32.0	32.0	32.0
4	31.569286287186024	32.0	32.0	32.0	32.0	32.0
5	31.493528473145492	32.0	32.0	32.0	32.0	32.0
6	35.05814030855737	36.0	36.0	36.0	36.0	36.0
7	35.085158736591495	36.0	36.0	36.0	36.0	36.0
8	35.01549319458373	36.0	36.0	36.0	36.0	36.0
9	35.159520505769876	36.0	36.0	36.0	36.0	36.0
10	35.01940913671441	36.0	36.0	36.0	36.0	36.0
11	35.168283893102156	36.0	36.0	36.0	36.0	36.0
12	35.07323549728352	36.0	36.0	36.0	36.0	36.0
13	35.12072037850478	36.0	36.0	36.0	36.0	36.0
14	35.078787013637445	36.0	36.0	36.0	36.0	36.0
15	35.04050828614323	36.0	36.0	36.0	36.0	36.0
16	35.04641183807331	36.0	36.0	36.0	36.0	36.0
17	35.020115627355224	36.0	36.0	36.0	36.0	36.0
18	35.04044175020959	36.0	36.0	36.0	36.0	36.0
19	35.014116505629545	36.0	36.0	36.0	36.0	36.0
20	34.999304397057415	36.0	36.0	36.0	36.0	36.0
21	34.992977434640544	36.0	36.0	36.0	36.0	36.0
22	34.97830928563393	36.0	36.0	36.0	36.0	36.0
23	34.929525139090345	36.0	36.0	36.0	32.0	36.0
24	34.91726801036993	36.0	36.0	36.0	32.0	36.0
25	34.88967737330651	36.0	36.0	36.0	32.0	36.0
26	34.84186223190555	36.0	36.0	36.0	32.0	36.0
27	34.815246648705994	36.0	36.0	36.0	32.0	36.0
28	34.79921995690891	36.0	36.0	36.0	32.0	36.0
29	34.780043091090114	36.0	36.0	36.0	32.0	36.0
30	34.75870320255595	36.0	36.0	36.0	32.0	36.0
31	34.75101648760435	36.0	36.0	36.0	32.0	36.0
32	34.705173229327585	36.0	36.0	36.0	32.0	36.0
33	34.676746658988826	36.0	36.0	36.0	32.0	36.0
34	34.65313729024547	36.0	36.0	36.0	32.0	36.0
35	34.62007497994849	36.0	36.0	36.0	32.0	36.0
36	34.57405993798851	36.0	36.0	36.0	32.0	36.0
37	34.55667107416821	36.0	36.0	36.0	32.0	36.0
38	34.51614948083826	36.0	36.0	36.0	32.0	36.0
39	34.48692810852857	36.0	36.0	36.0	32.0	36.0
40	34.4577321408481	36.0	36.0	36.0	32.0	36.0
41	34.423964549654556	36.0	36.0	36.0	32.0	36.0
42	34.37569575416061	36.0	36.0	36.0	32.0	36.0
43	34.37603206306155	36.0	36.0	36.0	32.0	36.0
44	34.34029621797656	36.0	36.0	36.0	32.0	36.0
45	34.26697361910718	36.0	36.0	36.0	32.0	36.0
46	34.27078552323253	36.0	36.0	36.0	32.0	36.0
47	34.219393168090335	36.0	36.0	36.0	32.0	36.0
48	34.1819564225927	36.0	36.0	36.0	32.0	36.0
49	34.166597509620495	36.0	36.0	36.0	32.0	36.0
50	33.61526140758582	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	9.0
21	25.0
22	88.0
23	198.0
24	635.0
25	1505.0
26	3165.0
27	5810.0
28	9513.0
29	14505.0
30	20886.0
31	29520.0
32	43178.0
33	69185.0
34	148074.0
35	480320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11218820477578	18.32223437515124	11.470361909924211	26.095215510148773
2	16.12033360674182	20.922523778069735	36.4014362413834	26.555706373805048
3	18.65492887887755	24.776136014458917	28.69995572319983	27.868979383463703
4	12.15566058849361	15.815992615703244	36.310077556891386	35.71826923891176
5	13.977143092178881	37.129349484225536	33.68472371255993	15.20878371103565
6	33.991841435442815	35.68367899968062	16.779617137824577	13.544862427051982
7	29.660630446117388	30.848720272047288	21.187097835646565	18.303551446188763
8	28.259262709270633	32.624624828065095	19.829885763850662	19.286226698813604
9	27.495067267828908	13.993837562800849	18.86414693069738	39.64694823867286
10	15.673930374379552	26.81252956312506	32.11266106232482	25.400879000170573
11	37.21705594220326	21.339767559740196	22.283489047580453	19.159687450476095
12	24.21133748114311	24.062176015368593	29.06604114824085	22.660445355247447
13	29.518485496981086	19.585759374610614	25.658191601713483	25.237563526694824
14	23.25285711347764	19.81803027022057	25.608350138697176	31.320762477604607
15	25.097717091629658	27.098634077769617	23.18837774506092	24.615271085539806
16	25.57096904143495	25.79477172730913	24.216297432559784	24.41796179869614
17	23.676872472390613	25.89747901396166	25.8036028603193	24.62204565332843
18	24.68664599616995	24.551154640397474	27.098634077769617	23.66356528566296
19	24.929441666737233	25.656739908615922	25.94175565343731	23.47206277120954
20	24.956418963466934	24.48788501622872	26.20439112967128	24.35130489063307
21	26.21781929082373	24.589140609783687	24.94758783045676	24.245452268935825
22	24.970693945592963	24.997913191172255	25.590083000552855	24.44130986268193
23	23.953057084201827	24.57232516473692	26.125273855854136	25.349343895207117
24	24.213152097515064	25.547983900723548	25.767310532880245	24.471553468881144
25	24.49465958401734	24.750157569188296	25.931351852904776	24.82383099388958
26	23.84309133206149	25.31414033759123	26.561991529370776	24.280776800976504
27	24.850566341769685	25.283049910418438	25.687709361363915	24.17867438644796
28	24.076330023069822	24.576559269604815	26.480696715907293	24.866413991418074
29	23.888335766935512	25.34680343228638	26.396256567399085	24.36860423337902
30	24.29710834832408	25.084893802601194	26.56852414830981	24.04947370076492
31	24.285373829118786	25.14804245234515	25.65069118737608	24.91589253115999
32	23.896199104547307	25.38406355512381	25.744083443319248	24.975653897009632
33	23.730706091425212	24.9616208637332	26.1996731271042	25.107999917737388
34	24.368120335679833	25.133283572519932	26.596590214862676	23.902005876937558
35	25.23296649855254	24.91274719611527	26.148621919839925	23.705664385492263
36	24.056732166252733	25.479028478589345	25.868324177585617	24.59591517757231
37	25.18022164934111	25.190625449873643	25.5497985170955	24.079354383689743
38	24.108630194490583	25.168850053410207	25.961595459103993	24.760924292995217
39	24.352756583730635	25.236716705721246	25.577622634798775	24.832904075749347
40	24.809435037338755	25.115984229773986	26.023534364599982	24.05104636828728
41	23.680622679559317	25.512417419833273	26.483116204403228	24.323843696204186
42	25.102072170922344	25.70137947136596	25.75267262747982	23.443875730231873
43	24.16294771122437	24.75124633901147	25.95506284016496	25.1307431095992
44	23.83377630135213	25.309422335024152	26.179712347012718	24.677089016611
45	24.155084373612574	25.403298488666508	25.90389065847589	24.537726479245023
46	23.849560680313424	25.2548029987213	26.053720033050293	24.84191628791499
47	24.240129394244764	24.888189387881507	26.320284628626663	24.55139658924707
48	24.692331794135402	26.076763111510598	25.1498570687171	24.0810480256369
49	23.620861313709668	25.586453767808948	25.93316646927673	24.859518449204653
50	23.438673829965605	26.308671083846164	25.298897560066823	24.953757526121404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	39.5
2	55.0
3	138.0
4	221.0
5	266.0
6	311.0
7	263.0
8	215.0
9	293.0
10	371.0
11	546.5
12	722.0
13	1227.5
14	1733.0
15	2284.5
16	2836.0
17	3362.5
18	3889.0
19	4119.5
20	4350.0
21	4980.5
22	5611.0
23	5803.0
24	5995.0
25	6807.0
26	7619.0
27	8946.5
28	10274.0
29	11468.0
30	12662.0
31	13593.0
32	14524.0
33	16618.5
34	18713.0
35	21326.0
36	23939.0
37	28526.5
38	33114.0
39	35122.5
40	37131.0
41	40536.0
42	43941.0
43	44789.0
44	45637.0
45	50057.5
46	54478.0
47	57939.5
48	61401.0
49	61878.5
50	62356.0
51	59015.0
52	55674.0
53	54340.0
54	53006.0
55	53499.5
56	53993.0
57	52897.5
58	51802.0
59	47344.0
60	42886.0
61	37929.5
62	32973.0
63	28754.5
64	24536.0
65	21169.0
66	17802.0
67	15365.5
68	12929.0
69	11717.0
70	10505.0
71	8277.0
72	6049.0
73	5441.0
74	4833.0
75	3586.5
76	2340.0
77	2120.0
78	1900.0
79	1639.5
80	1379.0
81	1047.5
82	716.0
83	645.5
84	575.0
85	467.5
86	360.0
87	260.5
88	161.0
89	104.0
90	47.0
91	35.0
92	23.0
93	14.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014154007701231883
2	0.00229851407114022
3	3.629232743905611E-4
4	2.419488495937074E-4
5	0.0
6	6.048721239842685E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.419488495937074E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	826621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.776298679275975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38947782243345	50.89716989988232
2	11.701888780927458	14.457987528844866
3	2.9219385843374894	5.415196521256006
4	1.0578650172443809	2.6140394107058516
5	0.5033967640755354	1.5548994425855647
6	0.29637306222655385	1.098529848755954
7	0.1800686756131967	0.7786783401223749
8	0.12140097713653328	0.5999762418833946
9	0.0910756798197517	0.5063686559066779
>10	0.566934299790262	7.077063603235949
>50	0.08311717965332022	3.657519005974933
>100	0.08173624157591497	9.430749033983336
>500	0.004726915166191255	1.9118224668629225
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	936	0.11323206160985506	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	921	0.11141744523790226	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	852	0.10307020992691934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.419488495937074E-4	0.0
12	0.0	0.0	0.0	2.419488495937074E-4	1.209744247968537E-4
13	0.0	0.0	0.0	2.419488495937074E-4	1.209744247968537E-4
14	0.0	0.0	0.0	2.419488495937074E-4	1.209744247968537E-4
15	0.0	0.0	0.0	3.629232743905611E-4	1.209744247968537E-4
16	0.0	0.0	0.0	3.629232743905611E-4	2.419488495937074E-4
17	0.0	0.0	0.0	6.048721239842685E-4	2.419488495937074E-4
18	0.0	0.0	0.0	7.258465487811222E-4	2.419488495937074E-4
19	0.0	0.0	0.0	8.468209735779759E-4	2.419488495937074E-4
20	0.0	0.0	0.0	0.001209744247968537	2.419488495937074E-4
21	0.0	0.0	0.0	0.0014516930975622443	3.629232743905611E-4
22	0.0	0.0	0.0	0.001572667522359098	3.629232743905611E-4
23	0.0	0.0	0.0	0.0038711815934993183	3.629232743905611E-4
24	0.0	0.0	0.0	0.007016516638217515	3.629232743905611E-4
25	0.0	0.0	0.0	0.008589184160576612	3.629232743905611E-4
26	0.0	0.0	0.0	0.011976468054888516	3.629232743905611E-4
27	0.0	0.0	0.0	0.015847649648387835	3.629232743905611E-4
28	0.0	0.0	0.0	0.023710987260183326	3.629232743905611E-4
29	0.0	0.0	0.0	0.040284483457352284	3.629232743905611E-4
30	0.0	0.0	0.0	0.06750372903664437	3.629232743905611E-4
31	0.0	0.0	0.0	0.12339391329279077	3.629232743905611E-4
32	0.0	0.0	0.0	0.18472794666479558	3.629232743905611E-4
33	0.0	0.0	0.0	0.24932828950631547	3.629232743905611E-4
34	0.0	0.0	0.0	0.3204612512868654	3.629232743905611E-4
35	0.0	0.0	0.0	0.40115119262636684	3.629232743905611E-4
36	0.0	0.0	0.0	0.511842791315488	3.629232743905611E-4
37	0.0	0.0	0.0	0.6754002136408342	3.629232743905611E-4
38	0.0	0.0	0.0	0.8777904263259704	3.629232743905611E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	25	0.0023524116	35.199852	2
CGTCTTA	230	0.0	22.956429	15
TCTCGTC	235	0.0	22.467993	12
TAGGACG	140	7.2759576E-12	21.99991	4
CCACGCG	50	0.0025798162	21.999908	9
GTACGAA	50	0.0025798162	21.999908	19
CTAGCGG	255	0.0	20.705797	29
TTCTCGT	260	0.0	20.30761	11
CGGTCCA	285	0.0	20.070093	10
CGCAATA	285	0.0	20.070093	36
ACGCATT	55	0.0044825394	19.99992	20
TCTAGCG	265	0.0	19.924446	28
GTTAGGC	100	1.1226002E-6	19.799917	16
CAATACG	280	0.0	19.642776	38
GTCTTAG	90	9.99799E-6	19.559023	1
TTACCCC	90	1.0013671E-5	19.555475	4
ATAGGGT	320	0.0	19.24992	3
CTCGTCT	290	0.0	18.965439	13
GTCGATA	105	1.7913608E-6	18.857065	36
TCGATAT	95	1.596171E-5	18.52624	37
>>END_MODULE