FastQCFastQC Report
Thu 2 Feb 2017
SRR4062657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4713
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT330.7001909611712286No Hit
GTCCTAAAGTGTGTATTTCTCATTT330.7001909611712286No Hit
CTTTAGGACGTGAAATATGGCGAGG250.5304476978569913No Hit
CTGTAGGACCTGGAATATGGCGAGA230.488011882028432No Hit
CTGTAGGACGTGGAATATGGCAAGA210.4455760661998727No Hit
GTCCTTCAGTGTGCATTTCTCATTT190.40314025037131335No Hit
GTACATGGAAGCAGTGGTATCAACG190.40314025037131335No Hit
CTGAAGGACCTGGAATATGGCGAGA140.29705071079991513No Hit
GTCCTACAGTGTGCATTTCTCATTT140.29705071079991513No Hit
TATCAACGCAGAGTACTTTTTTTTT140.29705071079991513No Hit
GTATCAACGCAGAGTACTTTTTTTT130.2758328028856355No Hit
ATTTAGAAATGTCCACTGTAGGACG130.2758328028856355No Hit
TTTCTAAATTTTCCACCTTTTTCAG120.2546148949713558No Hit
TCCATGTACTCTGCGTTGATACCAC100.21217907914279655No Hit
GATATACACTGTTCTACAAATCCCG90.19096117122851686No Hit
CATGTACTCTGCGTTGATACCACTG90.19096117122851686No Hit
ATACACACTTTAGGACGTGAAATAT90.19096117122851686No Hit
CTACAGTGGACATTTCTAAATTTTC90.19096117122851686No Hit
CTGTAGGACATGGAATATGGCAAGA80.1697432633142372No Hit
TTCCAGGTCCTTCAGTGTGCATTTC70.14852535539995756No Hit
GTGTATTTCTCATTTTCCGTGATTT70.14852535539995756No Hit
CCTAAAGTGTGTATTTCTCATTTTC70.14852535539995756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG60.1273074474856779No Hit
GTACTTTTTTTTTTTTTTTTTTTTT60.1273074474856779No Hit
GTGTATATCAATGAGTTACAATGAG60.1273074474856779No Hit
GTATCAACGCAGAGTACATGGGAAA60.1273074474856779No Hit
TCCTAAAGTGTGTATTTCTCATTTT60.1273074474856779No Hit
TTTTTCAAGTCGTCAAGTGGATGTT50.10608953957139827No Hit
ACACTGAAGGACCTGGAATATGGCG50.10608953957139827No Hit
CCACTGTAGGACGTGGAATATGGCA50.10608953957139827No Hit
GAGTACATGGAAGCAGTGGTATCAA50.10608953957139827No Hit
GGAATATGGCGAGAAAACTGAAAAT50.10608953957139827No Hit
GGTATCAACGCAGAGTACTTTTTTT50.10608953957139827No Hit
GTATATCAATGAGTTACAATGAGAA50.10608953957139827No Hit
CTCCGAGGTCACCCCAACCGAAATT50.10608953957139827No Hit
ACCTGGAATATGGCGAGAAAACTGA50.10608953957139827No Hit
GCTTCCATGTACTCTGCGTTGATAC50.10608953957139827No Hit
TTGTAGAACAGTGTATATCAATGAG50.10608953957139827No Hit
GAATATGGCAAGAAAACTGAAAATC50.10608953957139827No Hit
CCTTCAGTGTGCATTTCTCATTTTT50.10608953957139827No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)