##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062657_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4713
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.069382558879695	32.0	32.0	32.0	14.0	32.0
2	27.687884574580945	32.0	21.0	32.0	14.0	32.0
3	28.17525991937195	32.0	27.0	32.0	14.0	32.0
4	27.74050498620836	32.0	21.0	32.0	14.0	32.0
5	27.898578400169743	32.0	21.0	32.0	14.0	32.0
6	31.24867388075536	36.0	32.0	36.0	14.0	36.0
7	30.983874389985147	36.0	27.0	36.0	14.0	36.0
8	30.57606619987269	36.0	21.0	36.0	14.0	36.0
9	31.413324846170166	36.0	32.0	36.0	14.0	36.0
10	30.23912582219393	36.0	21.0	36.0	14.0	36.0
11	31.697008274984086	36.0	32.0	36.0	14.0	36.0
12	30.939528962444303	36.0	32.0	36.0	14.0	36.0
13	31.36940377678761	36.0	32.0	36.0	14.0	36.0
14	30.622533418204966	36.0	27.0	36.0	14.0	36.0
15	30.086356885211117	36.0	21.0	36.0	14.0	36.0
16	30.57436876723955	36.0	27.0	36.0	14.0	36.0
17	30.207511139401657	36.0	21.0	36.0	14.0	36.0
18	30.388075535752176	36.0	21.0	36.0	14.0	36.0
19	30.635476342032675	36.0	27.0	36.0	14.0	36.0
20	30.51368555060471	36.0	27.0	36.0	14.0	36.0
21	30.272650116698493	36.0	21.0	36.0	14.0	36.0
22	30.06131975387227	36.0	21.0	36.0	14.0	36.0
23	30.1060895395714	36.0	21.0	36.0	14.0	36.0
24	30.261192446424783	36.0	21.0	36.0	14.0	36.0
25	29.197538722681944	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	1.0
10	1.0
11	0.0
12	0.0
13	1.0
14	7.0
15	7.0
16	26.0
17	57.0
18	88.0
19	100.0
20	123.0
21	133.0
22	141.0
23	143.0
24	129.0
25	144.0
26	169.0
27	145.0
28	177.0
29	234.0
30	218.0
31	283.0
32	306.0
33	385.0
34	707.0
35	984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29280712921705	21.196690006365372	12.709526840653512	25.800976023764054
2	12.17907914279652	21.939316783365157	46.89157649055803	18.990027583280288
3	18.67175896456609	28.389560789306174	31.12667091024825	21.81200933587948
4	10.37555697008275	17.759388924252068	42.32972628898791	29.535327816677277
5	10.16553480475382	41.341256366723265	38.05178268251274	10.441426146010187
6	28.14689025684568	40.37359371683294	20.165569942687327	11.313946083634049
7	25.870858113848765	33.94222599830076	23.300764655904842	16.886151231945625
8	22.078198045048875	43.391415214619634	21.121971950701234	13.40841478963026
9	25.568544102019132	15.32412327311371	23.421891604675878	35.68544102019129
10	13.660505629912894	30.78393881453155	38.21967282770342	17.335882727852134
11	31.122448979591837	21.53486394557823	31.122448979591837	16.220238095238095
12	24.160645983850404	24.925626859328517	35.35911602209944	15.554611134721632
13	29.528061224489793	24.022108843537417	27.06207482993197	19.387755102040817
14	17.687074829931973	26.87074829931973	29.209183673469386	26.232993197278915
15	18.66496598639456	39.13690476190476	25.042517006802722	17.15561224489796
16	16.486084554918207	29.21181219460378	35.71276821754833	18.58933503292968
17	17.488312792180196	32.68168295792605	32.04419889502763	17.785805354866127
18	16.259298618490966	27.630180658873538	39.76620616365569	16.344314558979807
19	23.0605738575983	26.31243358129649	30.010626992561107	20.6163655685441
20	21.657810839532413	28.862911795961743	33.81509032943677	15.664187035069075
21	19.85122210414453	28.034006376195535	29.160467587672688	22.954303931987248
22	21.114182436742503	33.31915798426536	29.045290240272166	16.521369338719964
23	17.049319727891156	32.88690476190476	31.207482993197278	18.856292517006803
24	21.1734693877551	29.272959183673468	33.269557823129254	16.284013605442176
25	19.23894557823129	31.653911564625854	32.525510204081634	16.581632653061224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	2.5
3	5.0
4	5.0
5	5.0
6	8.0
7	11.0
8	11.0
9	11.0
10	12.5
11	14.0
12	14.0
13	14.0
14	27.0
15	40.0
16	40.0
17	40.0
18	65.5
19	91.0
20	91.0
21	91.0
22	148.0
23	205.0
24	205.0
25	205.0
26	286.5
27	368.0
28	368.0
29	368.0
30	497.0
31	626.0
32	626.0
33	626.0
34	675.0
35	724.0
36	724.0
37	724.0
38	700.5
39	677.0
40	677.0
41	677.0
42	646.5
43	616.0
44	616.0
45	616.0
46	610.5
47	605.0
48	605.0
49	605.0
50	510.5
51	416.0
52	416.0
53	416.0
54	291.5
55	167.0
56	167.0
57	167.0
58	122.5
59	78.0
60	78.0
61	78.0
62	59.0
63	40.0
64	40.0
65	40.0
66	29.5
67	19.0
68	19.0
69	19.0
70	10.0
71	1.0
72	1.0
73	1.0
74	1.5
75	2.0
76	2.0
77	2.0
78	1.0
79	0.0
80	0.0
81	0.0
82	0.5
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	3.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.02121790791427965
6	0.0424358158285593
7	0.10608953957139827
8	0.14852535539995756
9	0.1697432633142372
10	0.1273074474856779
11	0.19096117122851686
12	0.14852535539995756
13	0.19096117122851686
14	0.19096117122851686
15	0.19096117122851686
16	0.1273074474856779
17	0.14852535539995756
18	0.1697432633142372
19	0.1697432633142372
20	0.1697432633142372
21	0.1697432633142372
22	0.21217907914279655
23	0.19096117122851686
24	0.19096117122851686
25	0.19096117122851686
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4713.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.20835985571823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.4622200344573	81.4343305750053
2	3.1257691361063253	5.389348610227032
3	0.8368200836820083	2.1642266072565244
4	0.5906965296578882	2.0369191597708465
5	0.31996062023135613	1.3791640144281774
6	0.12306177701206006	0.6365372374283895
7	0.07383706620723603	0.4455760661998727
8	0.02461235540241201	0.1697432633142372
9	0.09844942160964804	0.7638446849140674
>10	0.34457297563376815	5.580309781455548
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	33	0.7001909611712286	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	33	0.7001909611712286	No Hit
CTTTAGGACGTGAAATATGGCGAGG	25	0.5304476978569913	No Hit
CTGTAGGACCTGGAATATGGCGAGA	23	0.488011882028432	No Hit
CTGTAGGACGTGGAATATGGCAAGA	21	0.4455760661998727	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	19	0.40314025037131335	No Hit
GTACATGGAAGCAGTGGTATCAACG	19	0.40314025037131335	No Hit
CTGAAGGACCTGGAATATGGCGAGA	14	0.29705071079991513	No Hit
GTCCTACAGTGTGCATTTCTCATTT	14	0.29705071079991513	No Hit
TATCAACGCAGAGTACTTTTTTTTT	14	0.29705071079991513	No Hit
GTATCAACGCAGAGTACTTTTTTTT	13	0.2758328028856355	No Hit
ATTTAGAAATGTCCACTGTAGGACG	13	0.2758328028856355	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	12	0.2546148949713558	No Hit
TCCATGTACTCTGCGTTGATACCAC	10	0.21217907914279655	No Hit
GATATACACTGTTCTACAAATCCCG	9	0.19096117122851686	No Hit
CATGTACTCTGCGTTGATACCACTG	9	0.19096117122851686	No Hit
ATACACACTTTAGGACGTGAAATAT	9	0.19096117122851686	No Hit
CTACAGTGGACATTTCTAAATTTTC	9	0.19096117122851686	No Hit
CTGTAGGACATGGAATATGGCAAGA	8	0.1697432633142372	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	7	0.14852535539995756	No Hit
GTGTATTTCTCATTTTCCGTGATTT	7	0.14852535539995756	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	7	0.14852535539995756	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	6	0.1273074474856779	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6	0.1273074474856779	No Hit
GTGTATATCAATGAGTTACAATGAG	6	0.1273074474856779	No Hit
GTATCAACGCAGAGTACATGGGAAA	6	0.1273074474856779	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	6	0.1273074474856779	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	5	0.10608953957139827	No Hit
ACACTGAAGGACCTGGAATATGGCG	5	0.10608953957139827	No Hit
CCACTGTAGGACGTGGAATATGGCA	5	0.10608953957139827	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5	0.10608953957139827	No Hit
GGAATATGGCGAGAAAACTGAAAAT	5	0.10608953957139827	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.10608953957139827	No Hit
GTATATCAATGAGTTACAATGAGAA	5	0.10608953957139827	No Hit
CTCCGAGGTCACCCCAACCGAAATT	5	0.10608953957139827	No Hit
ACCTGGAATATGGCGAGAAAACTGA	5	0.10608953957139827	No Hit
GCTTCCATGTACTCTGCGTTGATAC	5	0.10608953957139827	No Hit
TTGTAGAACAGTGTATATCAATGAG	5	0.10608953957139827	No Hit
GAATATGGCAAGAAAACTGAAAATC	5	0.10608953957139827	No Hit
CCTTCAGTGTGCATTTCTCATTTTT	5	0.10608953957139827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE