FastQCFastQC Report
Thu 2 Feb 2017
SRR4062657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4713
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA180.3819223424570337No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA150.31826861871419476No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA120.2546148949713558No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC120.2546148949713558No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA70.14852535539995756No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGA70.14852535539995756No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA60.1273074474856779No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCA60.1273074474856779No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA50.10608953957139827No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA50.10608953957139827No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC50.10608953957139827No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG50.10608953957139827No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAATATTTA50.10608953957139827No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA50.10608953957139827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAT250.002128096535.1829812
AAAAATG300.0052013929.31914913
Produced by FastQC (version 0.11.5)