##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4713
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.16380224909824	32.0	32.0	32.0	21.0	32.0
2	30.06153193295141	32.0	32.0	32.0	21.0	32.0
3	31.162741353702526	32.0	32.0	32.0	32.0	32.0
4	31.356885211118183	32.0	32.0	32.0	32.0	32.0
5	31.206238064926797	32.0	32.0	32.0	32.0	32.0
6	34.83407596011033	36.0	36.0	36.0	32.0	36.0
7	34.80140038192234	36.0	36.0	36.0	32.0	36.0
8	34.57012518565669	36.0	36.0	36.0	32.0	36.0
9	34.930617441120305	36.0	36.0	36.0	32.0	36.0
10	34.67027371101209	36.0	36.0	36.0	32.0	36.0
11	34.9484404837683	36.0	36.0	36.0	36.0	36.0
12	34.78187990664121	36.0	36.0	36.0	32.0	36.0
13	34.928283471249735	36.0	36.0	36.0	32.0	36.0
14	34.76766390833864	36.0	36.0	36.0	32.0	36.0
15	34.75429662635264	36.0	36.0	36.0	32.0	36.0
16	34.783577339274345	36.0	36.0	36.0	32.0	36.0
17	34.81667727562063	36.0	36.0	36.0	32.0	36.0
18	34.751326119244645	36.0	36.0	36.0	32.0	36.0
19	34.76766390833864	36.0	36.0	36.0	32.0	36.0
20	34.643751326119244	36.0	36.0	36.0	32.0	36.0
21	34.68215573944409	36.0	36.0	36.0	32.0	36.0
22	34.75938892425207	36.0	36.0	36.0	32.0	36.0
23	34.63971992361553	36.0	36.0	36.0	32.0	36.0
24	34.653723742838956	36.0	36.0	36.0	32.0	36.0
25	34.73986844897093	36.0	36.0	36.0	32.0	36.0
26	34.7010396774878	36.0	36.0	36.0	32.0	36.0
27	34.5786123488224	36.0	36.0	36.0	32.0	36.0
28	34.6098026734564	36.0	36.0	36.0	32.0	36.0
29	34.523657967324425	36.0	36.0	36.0	32.0	36.0
30	34.412476129853594	36.0	36.0	36.0	32.0	36.0
31	34.4551241247613	36.0	36.0	36.0	32.0	36.0
32	34.511563759813285	36.0	36.0	36.0	32.0	36.0
33	34.484404837683	36.0	36.0	36.0	32.0	36.0
34	34.41268830893274	36.0	36.0	36.0	32.0	36.0
35	34.31423721621048	36.0	36.0	36.0	32.0	36.0
36	34.420963293019305	36.0	36.0	36.0	32.0	36.0
37	34.36261404625504	36.0	36.0	36.0	32.0	36.0
38	34.34924676426904	36.0	36.0	36.0	32.0	36.0
39	33.91343093570974	36.0	36.0	36.0	32.0	36.0
40	34.0352217271377	36.0	36.0	36.0	32.0	36.0
41	34.034373010821135	36.0	36.0	36.0	32.0	36.0
42	34.14597920645024	36.0	36.0	36.0	32.0	36.0
43	33.91894759176745	36.0	36.0	36.0	32.0	36.0
44	33.81094844048377	36.0	36.0	36.0	32.0	36.0
45	33.888605983450034	36.0	36.0	36.0	32.0	36.0
46	33.69149161892637	36.0	36.0	36.0	21.0	36.0
47	33.78697220454063	36.0	36.0	36.0	27.0	36.0
48	33.694674305113516	36.0	36.0	36.0	27.0	36.0
49	33.478463823467	36.0	36.0	36.0	21.0	36.0
50	33.11754720984511	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	4.0
25	9.0
26	21.0
27	33.0
28	50.0
29	106.0
30	168.0
31	247.0
32	370.0
33	573.0
34	1021.0
35	2109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.91364311478888	23.636749416507534	12.900488011882027	24.549119456821558
2	12.01188455008489	24.490662139219015	45.73429541595925	17.763157894736842
3	20.050922978994272	27.66815192022067	32.86653935921918	19.41438574156588
4	11.648631444939529	20.517716953108422	42.56312327604498	25.270528325907065
5	11.775938892425208	40.20793549755994	36.19775090176109	11.818374708253767
6	26.967960959049435	41.67197114364524	20.072140886908553	11.287927010396775
7	24.824952259707192	33.842563123276044	23.870146403564608	17.462338213452153
8	21.34521536176533	44.53638871207299	21.090600466793973	13.027795459367706
9	24.90982389136431	16.210481646509653	21.790791427965203	37.08890303416083
10	14.343305750053045	30.08699342244855	37.492043284532144	18.077657542966264
11	30.36282622533418	22.745597284107788	29.705071079991512	17.186505410566518
12	24.039889666878846	24.549119456821558	35.624867388075536	15.78612348822406
13	28.55930405262041	23.80649267982177	27.625716104392104	20.00848716316571
14	18.31105453002334	27.201357946106512	28.0500742626777	26.437513261192446
15	18.990027583280288	39.48652662847444	23.530659876936134	17.992785911309145
16	17.398684489709314	30.829620199448332	34.01230638659028	17.759388924252068
17	17.63208147676639	33.09993634627626	31.232760449819647	18.035221727137703
18	17.50477402928071	28.516868236791854	37.279864205389345	16.698493528538087
19	22.915340547422026	28.219817525991935	29.492892000848713	19.371949925737322
20	21.91809887545088	30.723530659876936	31.253978357733924	16.104392106938256
21	20.241884150222788	28.79270103967749	27.859113091449185	23.106301718650542
22	21.621048164650965	32.633142372162105	28.898790579248885	16.847018883938045
23	18.014003819223426	32.50583492467642	31.08423509441969	18.39592616168046
24	21.599830256736684	29.83237852747719	32.145130490133674	16.422660725652452
25	19.159770846594526	31.65711860810524	32.50583492467642	16.677275620623806
26	15.998302567366856	31.996605134733713	34.160831741990236	17.844260555909187
27	17.63208147676639	32.9514109908763	31.466157436876724	17.950350095480587
28	16.889454699766603	33.2696796095905	29.344366645448762	20.496499045194145
29	19.732654360280076	30.956927646934012	27.922766815192023	21.387651177593888
30	16.54996817313813	31.27519626564821	31.487375344791	20.68746021642266
31	19.56291109696584	29.556545724591555	31.169106726076805	19.711436452365795
32	18.586887332908976	31.57224697644812	32.484617016762144	17.356248673880756
33	17.016762147252283	32.61192446424783	33.290897517504774	17.080415870995118
34	18.438361977509018	31.529811160619563	33.396987057076174	16.63483980479525
35	17.165287502652237	35.53999575641842	30.426479949077024	16.868236791852322
36	16.019520475281137	32.39974538510503	32.39974538510503	19.180988754508803
37	16.847018883938045	33.778909399533205	28.007638446849143	21.36643326967961
38	18.141311266709103	34.73371525567579	29.089751750477404	18.035221727137703
39	20.72989603225122	30.299172501591343	29.705071079991512	19.265860386165922
40	22.363674941650753	30.829620199448332	30.46891576490558	16.33778909399533
41	20.09335879482283	29.386802461277316	32.65436028007638	17.865478463823468
42	19.52047528113728	33.078718438361975	31.423721621048166	15.97708465945258
43	16.295353278166772	34.01230638659028	32.20878421387651	17.483556121366433
44	16.422660725652452	34.05474220241884	30.808402291534055	18.714194780394653
45	16.613621896880968	33.991088478676005	29.23827710587736	20.15701251856567
46	18.629323148737534	29.95968597496287	31.487375344791	19.92361553150859
47	21.111818374708253	30.40526204116274	31.08423509441969	17.398684489709314
48	17.14406959473796	30.808402291534055	30.935709739019735	21.111818374708253
49	19.56291109696584	29.344366645448762	31.423721621048166	19.669000636537238
50	17.335030765966476	34.18204964990451	28.53808614470613	19.944833439422872
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	1.5
8	1.0
9	1.5
10	2.0
11	3.5
12	5.0
13	5.5
14	6.0
15	8.5
16	11.0
17	12.0
18	13.0
19	22.0
20	31.0
21	39.0
22	47.0
23	49.5
24	52.0
25	82.5
26	113.0
27	143.0
28	173.0
29	201.0
30	229.0
31	305.5
32	382.0
33	451.5
34	521.0
35	571.0
36	621.0
37	583.5
38	546.0
39	509.0
40	472.0
41	412.5
42	353.0
43	304.5
44	256.0
45	237.5
46	219.0
47	207.5
48	196.0
49	162.5
50	129.0
51	106.5
52	84.0
53	84.5
54	85.0
55	66.5
56	48.0
57	41.5
58	35.0
59	32.5
60	30.0
61	25.0
62	20.0
63	17.0
64	14.0
65	9.5
66	5.0
67	3.5
68	2.0
69	2.0
70	2.0
71	1.5
72	1.0
73	1.5
74	2.0
75	1.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02121790791427965
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4713.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.90770210057289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.31723675385648	92.36155315085932
2	1.7661524703778224	3.3524294504561847
3	0.4247708473060586	1.20942075111394
4	0.1788508830762352	0.6789730532569488
5	0.1341381623071764	0.6365372374283895
6	0.0447127207690588	0.2546148949713558
7	0.0447127207690588	0.29705071079991513
8	0.0	0.0
9	0.0	0.0
>10	0.0894254415381176	1.20942075111394
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	18	0.3819223424570337	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	15	0.31826861871419476	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	12	0.2546148949713558	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	12	0.2546148949713558	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	7	0.14852535539995756	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGA	7	0.14852535539995756	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	6	0.1273074474856779	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCA	6	0.1273074474856779	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	5	0.10608953957139827	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	5	0.10608953957139827	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	5	0.10608953957139827	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	5	0.10608953957139827	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAATATTTA	5	0.10608953957139827	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	5	0.10608953957139827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.021217907914279653	0.0
23	0.0	0.0	0.0	0.042435815828559306	0.0
24	0.0	0.0	0.0	0.042435815828559306	0.0
25	0.0	0.0	0.0	0.042435815828559306	0.0
26	0.0	0.0	0.0	0.042435815828559306	0.0
27	0.0	0.0	0.0	0.042435815828559306	0.0
28	0.0	0.0	0.0	0.042435815828559306	0.0
29	0.0	0.0	0.0	0.042435815828559306	0.0
30	0.0	0.0	0.0	0.06365372374283895	0.0
31	0.0	0.0	0.0	0.1273074474856779	0.0
32	0.0	0.0	0.0	0.16974326331423722	0.0
33	0.0	0.0	0.0	0.21217907914279652	0.0
34	0.0	0.0	0.0	0.21217907914279652	0.0
35	0.0	0.0	0.0	0.29705071079991513	0.0
36	0.0	0.0	0.0	0.33948652662847445	0.0
37	0.0	0.0	0.0	0.42435815828559303	0.0
38	0.0	0.0	0.0	0.488011882028432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAAAT	25	0.0021280965	35.18298	12
AAAAATG	30	0.00520139	29.319149	13
>>END_MODULE