FastQCFastQC Report
Thu 2 Feb 2017
SRR4062656_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062656_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285095
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG84720.6592508725035892No Hit
TCCATGTACTCTGCGTTGATACCAC77880.6060252354884269No Hit
GTATCAACGCAGAGTACTTTTTTTT39450.3069811959427124No Hit
GAGTACATGGAAGCAGTGGTATCAA37690.2932857103949514No Hit
CATGTACTCTGCGTTGATACCACTG35140.2734428194024566No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34730.27025239379189864No Hit
TATCAACGCAGAGTACTTTTTTTTT27730.2157817126360308No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24820.1931374723269486No Hit
CCCATGTACTCTGCGTTGATACCAC24510.19072519930433157No Hit
GCTTCCATGTACTCTGCGTTGATAC23610.18372182601286285No Hit
GCGCAAGACGGACCAGAGCGAAAGC22690.17656282220380592No Hit
GCGTTGATACCACTGCTTCCATGTA22680.17648500694501185No Hit
GTACATGGGAAGCAGTGGTATCAAC21690.16878129632439626No Hit
GAATAGGACCGCGGTTCTATTTTGT20850.1622448145856921No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20330.15819842112839907No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20200.1571868227640758No Hit
CATGGAAGCAGTGGTATCAACGCAG19830.15430765818869424No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18950.1474599154148137No Hit
GAACTACGACGGTATCTGATCGTCT18390.14310226092234427No Hit
GTATCAACGCAGAGTACATGGAAGC18350.14279099988716787No Hit
GTATCTGATCGTCTTCGAACCTCCG17580.13679922496002242No Hit
GTCCTATTCCATTATTCCTAGCTGC17310.1346982129725818No Hit
GGGTAGGCACACGCTGAGCCAGTCA17080.13290846202031756No Hit
GTACATGGGGAATAATTGCAATCCC16780.13057400425649465No Hit
GAATAACGCCGCCGCATCGCCAGTC16480.12823954649267175No Hit
CTATTGGAGCTGGAATTACCGCGGC15950.1241153377765846No Hit
GATTAAGAGGGACGGCCGGGGGCAT15510.12069146638964434No Hit
CTCTTAATCATGGCCTCAGTTCCGA15070.11726759500270408No Hit
GTGCATGGCCGTTCTTAGTTGGTGG15030.11695633396752768No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG14830.11540002879164575No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC14620.11376590835696973No Hit
ACTCTGCGTTGATACCACTGCTTCC14540.11314338628661694No Hit
ACGCAGAGTACATGGAAGCAGTGGT14210.11057548274641174No Hit
CTTTAATATACGCTATTGGAGCTGG14130.10995296067605896No Hit
TCGTAGTTCCGACCATAAACGATGC13870.10792976394741244No Hit
CAGTGGTATCAACGCAGAGTACATG13780.10722942661826557No Hit
GTACATGGGGTGGTATCAACGCAAA13770.10715161135947147No Hit
GGTATCAACGCAGAGTACTTTTTTT13760.10707379610067737No Hit
ATCAGATACCGTCGTAGTTCCGACC13610.10590656721876593No Hit
GCTTTGAACACTCTAATTTTTTCAA13000.10115983643232603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTA508.7093074E-515.20056257
CAACGGA400.00527714614.25052614
TTATACA1101.8735591E-1013.8149234
CGGAATT500.001498441313.3010115
CAAGACG3750.013.1702264
AAGACGG3850.013.0753475
CGCAAGA3450.012.9374662
CGAACGA1550.012.87445416
CTTATAC604.0993106E-412.6636793
CGAGGTT1001.4460784E-712.3470884
TAGAGTG1850.012.3218985
GCGCAAG3700.012.3190181
GTTCAAA3850.012.0857041
CCGTCGT2800.011.8749759
TCGTTCA1052.7087117E-711.7650916
ACGAACG1701.8189894E-1211.73618515
GCAACAC1302.5993359E-911.6959319
TAGCACA658.037637E-411.689554
CGTTATT1302.6266207E-911.6881852
CGGACCA4000.011.6374779
Produced by FastQC (version 0.11.5)