##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062656_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1285095
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.018306039631312	32.0	32.0	32.0	32.0	32.0
2	30.716601496387426	32.0	32.0	32.0	32.0	32.0
3	30.71976390850482	32.0	32.0	32.0	32.0	32.0
4	30.750374096856653	32.0	32.0	32.0	32.0	32.0
5	30.59095553247036	32.0	32.0	32.0	32.0	32.0
6	34.18606017453963	36.0	36.0	36.0	32.0	36.0
7	34.03465580365654	36.0	36.0	36.0	32.0	36.0
8	34.01913477213747	36.0	36.0	36.0	32.0	36.0
9	34.171990397597064	36.0	36.0	36.0	32.0	36.0
10	33.870043070745744	36.0	36.0	36.0	32.0	36.0
11	34.198919924207935	36.0	36.0	36.0	32.0	36.0
12	33.95196074998346	36.0	36.0	36.0	32.0	36.0
13	34.103112999428056	36.0	36.0	36.0	32.0	36.0
14	34.00677459643062	36.0	36.0	36.0	32.0	36.0
15	33.96390850481871	36.0	36.0	36.0	32.0	36.0
16	33.951669720915575	36.0	36.0	36.0	32.0	36.0
17	33.86275255914932	36.0	36.0	36.0	32.0	36.0
18	33.89724417260981	36.0	36.0	36.0	32.0	36.0
19	33.886636396530996	36.0	36.0	36.0	32.0	36.0
20	33.866194328045786	36.0	36.0	36.0	32.0	36.0
21	33.824679887479135	36.0	36.0	36.0	32.0	36.0
22	33.78813550749167	36.0	36.0	36.0	32.0	36.0
23	33.749374948933735	36.0	36.0	36.0	32.0	36.0
24	33.74223150817644	36.0	36.0	36.0	32.0	36.0
25	33.234272174430686	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	46.0
5	112.0
6	224.0
7	44.0
8	158.0
9	229.0
10	138.0
11	46.0
12	87.0
13	74.0
14	311.0
15	358.0
16	550.0
17	806.0
18	1063.0
19	1619.0
20	2522.0
21	3649.0
22	5142.0
23	7067.0
24	9342.0
25	12656.0
26	17075.0
27	21337.0
28	28433.0
29	37461.0
30	48081.0
31	65108.0
32	93632.0
33	133637.0
34	289676.0
35	504410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25020136359144	17.288630486331012	11.235816026440695	25.225352123636856
2	16.36995943345351	20.334477517977913	36.382737156326336	26.912825892242243
3	19.425577877246607	24.658845428341976	27.69312897261425	28.22244772179716
4	12.433148421399977	15.123788266497321	35.03769837563894	37.40536493646376
5	14.558270054839198	36.69976219481343	32.89386727279096	15.848100477556407
6	34.52745172224272	34.433177169209976	17.04041739260408	13.998953715943221
7	30.046781294334384	30.494894391695233	21.00704366825148	18.451280645718903
8	28.933487533536596	31.501232433442755	20.171956590306415	19.393323442714237
9	27.26382720876293	14.60893458799926	18.72964296228681	39.397595240951
10	16.415691981222473	26.04930269806226	30.658148328765673	26.876856991949595
11	37.58356113586275	21.39242981487815	21.273237610827326	19.750771438431773
12	24.421152195017054	24.017801352242078	28.24219437817589	23.318852074564976
13	29.385717535391432	18.90041164061064	25.943252178953042	25.77061864504489
14	24.134962586133692	19.599802121352585	24.520592233652614	31.744643058861104
15	25.84587557031118	26.150924377341322	23.2423665742536	24.760833478093897
16	26.39501895234727	25.388667228563904	23.933940203124294	24.282373615964527
17	23.900449978809863	25.476700196943636	25.828285094607732	24.794564729638772
18	25.657288883002344	23.772720544124407	26.94413063972015	23.6258599331531
19	25.037593047662533	25.9615639331132	25.153371843157906	23.847471176066357
20	25.102444967965496	23.553305661506243	26.168885398748536	25.175363971779724
21	26.834927251124828	24.48873220233342	23.907364484525036	24.768976062016712
22	25.135784228924805	24.475915735073865	24.95538796495871	25.43291207104262
23	23.677802858471903	23.992121008591933	25.4787040492811	26.851372083655068
24	23.997145219060027	26.065848514290657	24.956893898606967	24.98011236804235
25	25.140376451192935	24.252734487301648	24.65795048433479	25.948938577170626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	324.0
1	324.0
2	737.5
3	1151.0
4	1151.0
5	1151.0
6	3136.0
7	5121.0
8	5121.0
9	5121.0
10	4926.0
11	4731.0
12	4731.0
13	4731.0
14	4191.5
15	3652.0
16	3652.0
17	3652.0
18	5891.5
19	8131.0
20	8131.0
21	8131.0
22	12944.0
23	17757.0
24	17757.0
25	17757.0
26	26368.0
27	34979.0
28	34979.0
29	34979.0
30	42958.5
31	50938.0
32	50938.0
33	50938.0
34	62846.5
35	74755.0
36	74755.0
37	74755.0
38	83803.0
39	92851.0
40	92851.0
41	92851.0
42	110391.0
43	127931.0
44	127931.0
45	127931.0
46	162447.5
47	196964.0
48	196964.0
49	196964.0
50	185518.0
51	174072.0
52	174072.0
53	174072.0
54	156018.0
55	137964.0
56	137964.0
57	137964.0
58	127955.0
59	117946.0
60	117946.0
61	117946.0
62	106953.0
63	95960.0
64	95960.0
65	95960.0
66	80389.5
67	64819.0
68	64819.0
69	64819.0
70	48899.5
71	32980.0
72	32980.0
73	32980.0
74	26097.0
75	19214.0
76	19214.0
77	19214.0
78	16359.5
79	13505.0
80	13505.0
81	13505.0
82	9351.5
83	5198.0
84	5198.0
85	5198.0
86	3847.5
87	2497.0
88	2497.0
89	2497.0
90	1690.0
91	883.0
92	883.0
93	883.0
94	523.5
95	164.0
96	164.0
97	164.0
98	386.0
99	608.0
100	608.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008248417432174275
2	0.0035795019045284588
3	0.0036573171633225558
4	0.012372626148261413
5	0.03346056128146169
6	0.04272057707795922
7	0.06388632746995358
8	0.08170602173380177
9	0.0887872102840646
10	0.11150926585194092
11	0.11314338628661694
12	0.12427096829417279
13	0.11290994051023466
14	0.11516658301526346
15	0.10629564351273642
16	0.10497278411323677
17	0.11508876775646938
18	0.1217808800127617
19	0.12551601243487837
20	0.11508876775646938
21	0.12216995630673219
22	0.14076780315852136
23	0.13088526529167105
24	0.12715013286955437
25	0.12310373941226135
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1285095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.339332907995825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.43654804961388	27.771689303801818
2	14.490992675172306	11.111499846815203
3	5.337266039392987	6.138816585084843
4	2.4850077418470384	3.8109415637448225
5	1.3365341490963916	2.5620913842555746
6	0.840938188808683	1.934460550546989
7	0.5409471632177371	1.4517687363368545
8	0.39524570661926367	1.2122765381225598
9	0.27728328783097833	0.9567770653777957
>10	1.5109989379104163	10.731479688624129
>50	0.15571063484115488	4.166531674315611
>100	0.15792947440858052	13.433396653761276
>500	0.02279394434676183	5.994907670095995
>1k	0.011396972173380916	7.454640495285774
>5k	4.070347204778899E-4	1.2687222438307568
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8472	0.6592508725035892	No Hit
TCCATGTACTCTGCGTTGATACCAC	7788	0.6060252354884269	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3945	0.3069811959427124	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3769	0.2932857103949514	No Hit
CATGTACTCTGCGTTGATACCACTG	3514	0.2734428194024566	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3473	0.27025239379189864	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2773	0.2157817126360308	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2482	0.1931374723269486	No Hit
CCCATGTACTCTGCGTTGATACCAC	2451	0.19072519930433157	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2361	0.18372182601286285	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2269	0.17656282220380592	No Hit
GCGTTGATACCACTGCTTCCATGTA	2268	0.17648500694501185	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2169	0.16878129632439626	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2085	0.1622448145856921	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2033	0.15819842112839907	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2020	0.1571868227640758	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1983	0.15430765818869424	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1895	0.1474599154148137	No Hit
GAACTACGACGGTATCTGATCGTCT	1839	0.14310226092234427	No Hit
GTATCAACGCAGAGTACATGGAAGC	1835	0.14279099988716787	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1758	0.13679922496002242	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1731	0.1346982129725818	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1708	0.13290846202031756	No Hit
GTACATGGGGAATAATTGCAATCCC	1678	0.13057400425649465	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1648	0.12823954649267175	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1595	0.1241153377765846	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1551	0.12069146638964434	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1507	0.11726759500270408	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1503	0.11695633396752768	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1483	0.11540002879164575	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1462	0.11376590835696973	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1454	0.11314338628661694	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1421	0.11057548274641174	No Hit
CTTTAATATACGCTATTGGAGCTGG	1413	0.10995296067605896	No Hit
TCGTAGTTCCGACCATAAACGATGC	1387	0.10792976394741244	No Hit
CAGTGGTATCAACGCAGAGTACATG	1378	0.10722942661826557	No Hit
GTACATGGGGTGGTATCAACGCAAA	1377	0.10715161135947147	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1376	0.10707379610067737	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1361	0.10590656721876593	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1300	0.10115983643232603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.781525879409694E-5
2	0.0	0.0	0.0	0.0	7.781525879409694E-5
3	0.0	0.0	0.0	0.0	7.781525879409694E-5
4	0.0	0.0	0.0	0.0	7.781525879409694E-5
5	0.0	0.0	0.0	0.0	1.5563051758819387E-4
6	0.0	0.0	0.0	0.0	1.5563051758819387E-4
7	0.0	0.0	0.0	0.0	1.5563051758819387E-4
8	0.0	0.0	0.0	0.0	1.5563051758819387E-4
9	0.0	0.0	0.0	0.0	1.5563051758819387E-4
10	0.0	0.0	0.0	1.5563051758819387E-4	1.5563051758819387E-4
11	0.0	0.0	0.0	1.5563051758819387E-4	1.5563051758819387E-4
12	0.0	0.0	0.0	1.5563051758819387E-4	4.668915527645816E-4
13	0.0	0.0	0.0	1.5563051758819387E-4	6.225220703527755E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTGTA	50	8.7093074E-5	15.2005625	7
CAACGGA	40	0.005277146	14.250526	14
TTATACA	110	1.8735591E-10	13.814923	4
CGGAATT	50	0.0014984413	13.30101	15
CAAGACG	375	0.0	13.170226	4
AAGACGG	385	0.0	13.075347	5
CGCAAGA	345	0.0	12.937466	2
CGAACGA	155	0.0	12.874454	16
CTTATAC	60	4.0993106E-4	12.663679	3
CGAGGTT	100	1.4460784E-7	12.347088	4
TAGAGTG	185	0.0	12.321898	5
GCGCAAG	370	0.0	12.319018	1
GTTCAAA	385	0.0	12.085704	1
CCGTCGT	280	0.0	11.874975	9
TCGTTCA	105	2.7087117E-7	11.76509	16
ACGAACG	170	1.8189894E-12	11.736185	15
GCAACAC	130	2.5993359E-9	11.69593	19
TAGCACA	65	8.037637E-4	11.68955	4
CGTTATT	130	2.6266207E-9	11.688185	2
CGGACCA	400	0.0	11.637477	9
>>END_MODULE