##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1285095
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21273213264389	32.0	32.0	32.0	32.0	32.0
2	31.379385181640266	32.0	32.0	32.0	32.0	32.0
3	31.479103879479727	32.0	32.0	32.0	32.0	32.0
4	31.5935794629969	32.0	32.0	32.0	32.0	32.0
5	31.514067053408503	32.0	32.0	32.0	32.0	32.0
6	35.111827530260406	36.0	36.0	36.0	36.0	36.0
7	35.11353557519094	36.0	36.0	36.0	36.0	36.0
8	35.05437341208238	36.0	36.0	36.0	36.0	36.0
9	35.183340531244774	36.0	36.0	36.0	36.0	36.0
10	35.063590629486534	36.0	36.0	36.0	36.0	36.0
11	35.18961166295099	36.0	36.0	36.0	36.0	36.0
12	35.09311762943596	36.0	36.0	36.0	36.0	36.0
13	35.13917336850583	36.0	36.0	36.0	36.0	36.0
14	35.0911854765601	36.0	36.0	36.0	36.0	36.0
15	35.06586050058556	36.0	36.0	36.0	36.0	36.0
16	35.076025507841834	36.0	36.0	36.0	36.0	36.0
17	35.04028651578288	36.0	36.0	36.0	36.0	36.0
18	35.05638182391185	36.0	36.0	36.0	36.0	36.0
19	35.02740030892658	36.0	36.0	36.0	36.0	36.0
20	35.015791828619676	36.0	36.0	36.0	36.0	36.0
21	34.99257875876881	36.0	36.0	36.0	36.0	36.0
22	34.9848874985896	36.0	36.0	36.0	36.0	36.0
23	34.934919208307555	36.0	36.0	36.0	32.0	36.0
24	34.90705122967563	36.0	36.0	36.0	32.0	36.0
25	34.87671884179769	36.0	36.0	36.0	32.0	36.0
26	34.83692489660297	36.0	36.0	36.0	32.0	36.0
27	34.819983736610915	36.0	36.0	36.0	32.0	36.0
28	34.79818846077527	36.0	36.0	36.0	32.0	36.0
29	34.76274827931009	36.0	36.0	36.0	32.0	36.0
30	34.746534691987755	36.0	36.0	36.0	32.0	36.0
31	34.73199491088207	36.0	36.0	36.0	32.0	36.0
32	34.68846272065489	36.0	36.0	36.0	32.0	36.0
33	34.64811550897016	36.0	36.0	36.0	32.0	36.0
34	34.62267069749707	36.0	36.0	36.0	32.0	36.0
35	34.560286982674434	36.0	36.0	36.0	32.0	36.0
36	34.533443831000824	36.0	36.0	36.0	32.0	36.0
37	34.506676938280826	36.0	36.0	36.0	32.0	36.0
38	34.436162307066795	36.0	36.0	36.0	32.0	36.0
39	34.381059766009514	36.0	36.0	36.0	32.0	36.0
40	34.3139254296375	36.0	36.0	36.0	32.0	36.0
41	34.29125940105595	36.0	36.0	36.0	32.0	36.0
42	34.188200872309054	36.0	36.0	36.0	32.0	36.0
43	34.20598710601162	36.0	36.0	36.0	32.0	36.0
44	34.16411082449157	36.0	36.0	36.0	32.0	36.0
45	34.04553282053078	36.0	36.0	36.0	32.0	36.0
46	34.0887397429762	36.0	36.0	36.0	32.0	36.0
47	34.03371423902513	36.0	36.0	36.0	32.0	36.0
48	33.9833257463456	36.0	36.0	36.0	32.0	36.0
49	33.98587030530817	36.0	36.0	36.0	32.0	36.0
50	33.41539963971535	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	25.0
22	125.0
23	341.0
24	980.0
25	2350.0
26	4853.0
27	9081.0
28	15490.0
29	23906.0
30	34018.0
31	48907.0
32	71747.0
33	110167.0
34	229443.0
35	733656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09713275289499	17.64258602480677	11.775784872024165	25.48449635027407
2	15.866006040264802	20.9364978448192	36.07762541798213	27.11987069693388
3	18.842571337416057	25.051708440653965	28.098810200063806	28.006910021866172
4	12.251331226611613	15.45242250949931	35.38288668602195	36.913359577867126
5	14.416599551005957	36.84638100685164	32.76987304440528	15.967146397737134
6	34.70955341649223	34.595787065497355	16.791664397046123	13.90299512096429
7	30.072298269463765	30.546583009945582	20.938173346209187	18.442945374381463
8	29.074037327979646	31.556110637734953	19.906076982635525	19.46377505164988
9	27.58683210190686	14.207432135367423	18.66251133184706	39.543224430878645
10	16.522358269233013	26.10943159844214	30.626062664627906	26.74214746769694
11	37.89011707305686	21.13773689882849	21.31243215482124	19.659713873293413
12	24.525346375170706	23.895820931526462	28.208887280706875	23.369945412595953
13	29.598434356993064	18.83456086904081	25.90983545963528	25.657169314330847
14	24.354930958411636	19.480972223843374	24.49896700243951	31.66512981530548
15	25.8594889871955	26.05947420229633	23.39033301040001	24.690703800108164
16	26.61865465199071	25.273540088475947	23.819717608425837	24.288087651107507
17	24.126543173850962	25.33431380559414	25.707671417288214	24.83147160326668
18	25.98811760998214	23.438033764040792	26.993490753601872	23.580357872375192
19	25.336648263357965	25.711639995486713	25.33579229551123	23.615919445644096
20	25.23214237079749	23.262949431754073	26.436800392188903	25.068107805259533
21	27.13130157692622	24.21346281792397	24.044448075823187	24.610787529326625
22	25.318828569094116	24.221555604838553	25.158762581754658	25.300853244312677
23	23.76905987495088	23.71497827008898	25.681058598780638	26.834903256179505
24	24.138059832152486	25.8331096144643	25.00173138950817	25.027099163875043
25	25.322719332033817	23.927569557114456	24.876371007590876	25.87334010326085
26	24.09502799403935	24.854271474093355	26.05784008186165	24.99286045000564
27	25.8436924896603	24.54153194899988	25.1713686536793	24.443406907660524
28	24.518809893432003	23.784545111450907	26.20047545123123	25.49616954388586
29	23.97060139522759	24.72898890743486	26.538504935432787	24.761904761904763
30	24.883063119847172	24.58409689556025	26.41719094697279	24.11564903761979
31	24.35384154478852	24.801045837078192	24.937845062038217	25.907267556095075
32	24.27579284021804	24.77272108287714	25.04772020745548	25.90376586944934
33	23.963675837194913	24.157435831592217	25.64261786093635	26.23627047027652
34	24.535773619849117	24.878472019578318	26.257903112221275	24.32785124835129
35	26.339375688178695	24.227703010283285	25.560133686614606	23.87278761492341
36	24.523556624218443	25.446523408775228	25.02079612791272	25.009123839093604
37	26.065621607741065	25.075655885362565	25.180628669475798	23.67809383742058
38	24.669849310751346	24.526591419311412	25.253619382224663	25.54993988771258
39	25.261478723362863	24.424653430291148	25.031301187849923	25.282566658496066
40	25.901042335391544	24.78104731556811	24.833806061030508	24.484104288009835
41	23.4985740353826	25.458973850182282	26.191837957505086	24.850614156930032
42	26.75420883281003	25.460763601134545	24.879172356907468	22.905855209147962
43	25.255020056882955	23.44861663923679	25.0540232434178	26.242340060462453
44	24.48021352507013	24.69047035433178	25.666584960644933	25.162731159953154
45	25.162108637882802	24.860185433761707	25.046319532797185	24.931386395558306
46	24.21163293240256	24.670043335385067	25.921470274913954	25.196853457298424
47	25.16039670218933	24.20404717160988	26.103984530326553	24.531571595874237
48	25.82797380738389	26.38660955026671	23.65116975787782	24.134246884471576
49	24.080865616938826	25.301320135865442	25.906956295059896	24.710857952135832
50	24.31440812889952	26.362429518774498	23.82253749531163	25.500624857014355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	27.5
2	39.0
3	164.0
4	289.0
5	311.0
6	333.0
7	316.0
8	299.0
9	412.0
10	525.0
11	770.5
12	1016.0
13	1780.0
14	2544.0
15	3546.5
16	4549.0
17	5155.0
18	5761.0
19	6085.0
20	6409.0
21	7160.0
22	7911.0
23	8171.0
24	8431.0
25	8478.0
26	8525.0
27	10301.0
28	12077.0
29	13451.0
30	14825.0
31	15629.0
32	16433.0
33	18922.0
34	21411.0
35	24764.0
36	28117.0
37	35700.5
38	43284.0
39	47215.0
40	51146.0
41	56281.0
42	61416.0
43	62997.0
44	64578.0
45	77504.0
46	90430.0
47	100971.0
48	111512.0
49	111064.0
50	110616.0
51	98882.0
52	87148.0
53	84561.5
54	81975.0
55	85333.5
56	88692.0
57	87482.0
58	86272.0
59	78945.5
60	71619.0
61	63115.0
62	54611.0
63	47780.5
64	40950.0
65	35268.0
66	29586.0
67	25603.5
68	21621.0
69	20420.5
70	19220.0
71	14789.0
72	10358.0
73	9515.5
74	8673.0
75	6094.0
76	3515.0
77	3218.0
78	2921.0
79	2516.0
80	2111.0
81	1690.5
82	1270.0
83	1113.5
84	957.0
85	825.5
86	694.0
87	489.5
88	285.0
89	180.5
90	76.0
91	51.0
92	26.0
93	18.0
94	10.0
95	7.5
96	5.0
97	6.5
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015874312793995773
2	0.004046393457293041
3	3.890762939704847E-4
4	1.5563051758819385E-4
5	0.0
6	3.890762939704847E-4
7	1.5563051758819385E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5563051758819385E-4
47	0.0
48	0.0
49	0.0
50	7.781525879409692E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1285095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.69803154993647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06847522746173	30.468115853404516
2	14.492498754986883	12.086173406457162
3	5.107441157473964	6.389107275713803
4	2.370549700879265	3.953890248718243
5	1.2829600241068908	2.6748453781258195
6	0.7835227693932665	1.9602814294952413
7	0.5159364417776339	1.505947381890402
8	0.36510191957092497	1.217922508896864
9	0.2646066213628636	0.9930217721319703
>10	1.443553005609416	11.016774687275882
>50	0.13629421869899197	3.9757809051989335
>100	0.1418830841261387	12.865683778041086
>500	0.018555381935436602	5.264754497732217
>1k	0.008434264516107548	5.179957593895755
>5k	1.874281003579455E-4	0.4477432830222058
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5729	0.4458036176313814	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4313	0.33561721117894006	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2157	0.1678475132188671	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2110	0.16419019605554452	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2075	0.16146666199775114	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2014	0.15671993121131123	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2008	0.15625303965854664	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1976	0.15376295137713555	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1805	0.14045654212334496	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1774	0.13804426910072795	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1710	0.13306409253790577	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1640	0.12761702442231898	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1620	0.12606071924643703	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1555	0.12100272742482072	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1504	0.11703414922632178	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1503	0.11695633396752768	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1488	0.11578910508561624	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1478	0.11501095249767526	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1461	0.11368809309817561	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1451	0.11290994051023466	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1382	0.10754068765344196	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	1356	0.10551749092479544	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1351	0.10512841463082495	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1344	0.10458370781926628	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1343	0.10450589256047217	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1336	0.1039611857489135	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1311	0.10201580427906108	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1311	0.10201580427906108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5563051758819387E-4
2	0.0	0.0	0.0	0.0	1.5563051758819387E-4
3	0.0	0.0	0.0	0.0	1.5563051758819387E-4
4	0.0	0.0	0.0	0.0	1.5563051758819387E-4
5	0.0	0.0	0.0	0.0	1.5563051758819387E-4
6	0.0	0.0	0.0	0.0	1.5563051758819387E-4
7	0.0	0.0	0.0	1.5563051758819387E-4	1.5563051758819387E-4
8	0.0	0.0	0.0	2.334457763822908E-4	1.5563051758819387E-4
9	0.0	0.0	0.0	3.1126103517638775E-4	1.5563051758819387E-4
10	0.0	0.0	0.0	4.668915527645816E-4	1.5563051758819387E-4
11	0.0	0.0	0.0	4.668915527645816E-4	1.5563051758819387E-4
12	0.0	0.0	0.0	4.668915527645816E-4	5.447068115586785E-4
13	0.0	0.0	0.0	4.668915527645816E-4	6.225220703527755E-4
14	0.0	0.0	0.0	4.668915527645816E-4	6.225220703527755E-4
15	7.781525879409694E-5	0.0	0.0	4.668915527645816E-4	8.559678467350663E-4
16	7.781525879409694E-5	0.0	0.0	4.668915527645816E-4	8.559678467350663E-4
17	7.781525879409694E-5	0.0	0.0	5.447068115586785E-4	8.559678467350663E-4
18	7.781525879409694E-5	0.0	0.0	6.225220703527755E-4	8.559678467350663E-4
19	7.781525879409694E-5	0.0	0.0	6.225220703527755E-4	8.559678467350663E-4
20	7.781525879409694E-5	0.0	0.0	7.003373291468724E-4	9.337831055291632E-4
21	7.781525879409694E-5	0.0	0.0	8.559678467350663E-4	9.337831055291632E-4
22	7.781525879409694E-5	0.0	0.0	0.0014784899170878418	9.337831055291632E-4
23	7.781525879409694E-5	0.0	0.0	0.003423871386940265	9.337831055291632E-4
24	7.781525879409694E-5	0.0	0.0	0.00583614440955727	9.337831055291632E-4
25	7.781525879409694E-5	0.0	0.0	0.007859341138203791	9.337831055291632E-4
26	7.781525879409694E-5	0.0	0.0	0.010193798902026699	0.0010115983643232602
27	7.781525879409694E-5	0.0	0.0	0.012606071924643704	0.0010115983643232602
28	7.781525879409694E-5	0.0	0.0	0.018597846851789168	0.0010115983643232602
29	7.781525879409694E-5	0.0	0.0	0.028558199977433574	0.0010115983643232602
30	7.781525879409694E-5	0.0	0.0	0.04435469751263525	0.0010115983643232602
31	7.781525879409694E-5	0.0	0.0	0.08038316233430214	0.0010115983643232602
32	7.781525879409694E-5	0.0	0.0	0.1216252494951735	0.001089413623117357
33	7.781525879409694E-5	0.0	0.0	0.15897657371634002	0.001089413623117357
34	7.781525879409694E-5	0.0	0.0	0.20621043580435688	0.001167228881911454
35	7.781525879409694E-5	0.0	0.0	0.26239305265369484	0.001167228881911454
36	7.781525879409694E-5	0.0	0.0	0.3410642792945269	0.001167228881911454
37	7.781525879409694E-5	0.0	0.0	0.45490800291029065	0.001167228881911454
38	7.781525879409694E-5	0.0	0.0	0.6029904403954571	0.001167228881911454
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	155	0.0	25.548365	35
TAACGGC	165	0.0	23.999979	36
TGGTATA	315	0.0	23.746012	44
CGACAGT	60	2.8729308E-4	21.999983	6
ATATAGC	50	0.002580539	21.99998	3
GGTGCGA	75	5.4730175E-5	20.533316	10
GTATATA	130	1.6243575E-9	20.308466	1
CCTTATA	55	0.0044837864	19.99998	2
ACCGTTA	55	0.0044837864	19.99998	8
ACGAATT	70	8.123752E-4	18.857126	21
CGCAATA	560	0.0	18.46427	36
GTCTTAG	185	2.1827873E-11	17.838514	1
GTACGAA	75	0.0012909382	17.599985	19
ATACCGT	405	0.0	17.3827	6
GACGGAC	775	0.0	17.316114	7
TAGCGGC	605	0.0	17.090893	30
GACAGTC	245	0.0	17.06121	7
CTAGCGG	600	0.0	16.866652	29
TACCGTC	420	0.0	16.761889	7
CAATACG	605	0.0	16.727257	38
>>END_MODULE