FastQCFastQC Report
Thu 2 Feb 2017
SRR4062655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101414
Sequences flagged as poor quality0
Sequence length25
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC1850.18242057309641668No Hit
GAATAGGACCGCGGTTCTATTTTGT1490.14692251562900585No Hit
TCGTAGTTCCGACCATAAACGATGC1450.14297828702151577No Hit
GATTAAGAGGGACGGCCGGGGGCAT1450.14297828702151577No Hit
GTATCTGATCGTCTTCGAACCTCCG1330.1311456011990455No Hit
ATCAGATACCGTCGTAGTTCCGACC1320.13015954404717298No Hit
GAACTACGACGGTATCTGATCGTCT1300.12818742974342795No Hit
GAATAACGCCGCCGCATCGCCAGTC1210.11931291537657523No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG1170.11536868676908514No Hit
CTATTGGAGCTGGAATTACCGCGGC1150.11339657246534009No Hit
CATCTAAGGGCATCACAGACCTGTT1140.11241051531346757No Hit
GTATCAACGCAGAGTACTTTTTTTT1070.10550811525035991No Hit
ATATTAAAGTTGCTGCAGTTAAAAA1040.10254994379474233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG350.002148813316.2886753
GTATCAA852.6101407E-713.4142041
TAAGAGG550.003030412112.0931084
AAGAGGG700.001468436210.8591175
CATGGGA951.6067189E-410.0018194
TATCAAC1356.5891494E-58.445982
ATCAACG1401.0074246E-48.1443383
TCAACGC1401.0074246E-48.1443384
CAACGCA1451.5149401E-47.86349875
AACGCAG1451.5149401E-47.86349876
CGCAGAG1604.7140618E-47.12629568
CAGAGTA1751.6946162E-47.058426410
ACATGGG2207.831235E-66.91034753
ACGCAGA1656.702135E-46.9103477
AGAGTAC1802.4097285E-46.85897311
GCAGAGT1709.415639E-46.7071029
GTACATG2301.5117987E-56.6098971
TACATGG2402.826195E-56.3344852
Produced by FastQC (version 0.11.5)