##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062655_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101414
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.253180034314788	32.0	14.0	32.0	14.0	32.0
2	22.13893545269884	21.0	14.0	32.0	14.0	32.0
3	22.853087344942512	21.0	14.0	32.0	14.0	32.0
4	21.626432248013096	21.0	14.0	32.0	14.0	32.0
5	22.323347861242038	21.0	14.0	32.0	14.0	32.0
6	26.01546137614136	21.0	14.0	36.0	14.0	36.0
7	25.556422190230144	21.0	14.0	36.0	14.0	36.0
8	25.066489833750765	21.0	14.0	36.0	14.0	36.0
9	25.385873745242275	27.0	14.0	36.0	14.0	36.0
10	23.17791429191236	21.0	14.0	36.0	14.0	36.0
11	26.58253298361173	32.0	14.0	36.0	14.0	36.0
12	24.49233833592995	21.0	14.0	36.0	14.0	36.0
13	25.185674561697596	21.0	14.0	36.0	14.0	36.0
14	24.233863174709604	21.0	14.0	36.0	14.0	36.0
15	24.23452383300136	21.0	14.0	36.0	14.0	36.0
16	24.414656753505433	21.0	14.0	36.0	14.0	36.0
17	24.06365984972489	21.0	14.0	36.0	14.0	36.0
18	24.323446466957225	21.0	14.0	36.0	14.0	36.0
19	24.296960971857928	21.0	14.0	36.0	14.0	36.0
20	24.2046857435857	21.0	14.0	36.0	14.0	36.0
21	24.24697773482951	21.0	14.0	36.0	14.0	36.0
22	24.01694046186917	21.0	14.0	36.0	14.0	36.0
23	23.850691226063464	21.0	14.0	36.0	14.0	36.0
24	23.877285187449466	21.0	14.0	36.0	14.0	36.0
25	23.353905772378567	21.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	8.0
4	10.0
5	14.0
6	18.0
7	14.0
8	10.0
9	8.0
10	8.0
11	6.0
12	7.0
13	7.0
14	308.0
15	1000.0
16	2607.0
17	5398.0
18	8229.0
19	9808.0
20	9830.0
21	8702.0
22	7238.0
23	5883.0
24	4863.0
25	3994.0
26	3618.0
27	3313.0
28	3064.0
29	2807.0
30	2818.0
31	2891.0
32	3157.0
33	3236.0
34	4291.0
35	4249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39277578913114	19.293159384275558	11.726769813330177	27.587295013263123
2	16.143219177406788	20.868544832414628	37.41014288390806	25.578093106270522
3	18.243136636162827	24.770235089933735	28.845850426001896	28.140777847901543
4	11.90335305719921	15.856015779092703	37.150887573964496	35.08974358974359
5	14.271905945476782	36.34848305519391	34.13323075709157	15.24638024223774
6	32.72998086369824	36.73084890212866	17.03229497524118	13.506875258931919
7	28.529568460309	31.08585410130429	22.20544998914738	18.17912744923933
8	26.81179442646246	33.19550801294703	20.299794742243627	19.692902818346887
9	26.5270471659939	13.91885356730456	19.42500715533492	40.129092111366624
10	15.227584573013633	26.548078536667223	32.32085920456452	25.903477685754616
11	36.80802337518755	21.373292268814655	22.798704888257127	19.01997946774066
12	23.319116166103903	24.349860790237546	29.62600951760362	22.705013526054934
13	27.964185940908777	19.997828254410123	25.768748580961688	26.26923722371941
14	22.552048884984057	20.329914411790835	25.647834628179943	31.47020207504516
15	24.373198562798596	27.801358234295414	22.92711335728669	24.898329845619298
16	24.759385210704522	26.109789443550536	24.591571819195877	24.539253526549064
17	22.881372665166055	26.645737076965602	26.205425897405522	24.26746436046282
18	24.04241036171221	25.372176591375773	27.261688516821987	23.323724530090033
19	24.43553721456003	25.737725957883722	25.997373902913388	23.82936292464286
20	24.669542641092214	24.73469629512631	25.55997591289154	25.03578515088994
21	25.96673083567797	24.939039439261563	24.610296658275335	24.48393306678513
22	24.655397132588174	25.546032623721317	25.367313085034954	24.431257158655555
23	23.156138306905472	25.158468434667565	26.328469027072927	25.35692423135404
24	23.44055446952195	25.710364709831566	25.889066603479254	24.960014217167227
25	23.61193381510879	24.92003317142519	26.079058563361375	25.388974450104644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	8.0
2	25.0
3	42.0
4	42.0
5	42.0
6	109.5
7	177.0
8	177.0
9	177.0
10	228.5
11	280.0
12	280.0
13	280.0
14	369.5
15	459.0
16	459.0
17	459.0
18	732.0
19	1005.0
20	1005.0
21	1005.0
22	1503.0
23	2001.0
24	2001.0
25	2001.0
26	2864.5
27	3728.0
28	3728.0
29	3728.0
30	4477.5
31	5227.0
32	5227.0
33	5227.0
34	6409.5
35	7592.0
36	7592.0
37	7592.0
38	8328.5
39	9065.0
40	9065.0
41	9065.0
42	9990.0
43	10915.0
44	10915.0
45	10915.0
46	12006.5
47	13098.0
48	13098.0
49	13098.0
50	12874.0
51	12650.0
52	12650.0
53	12650.0
54	11460.5
55	10271.0
56	10271.0
57	10271.0
58	9312.5
59	8354.0
60	8354.0
61	8354.0
62	7518.0
63	6682.0
64	6682.0
65	6682.0
66	5555.0
67	4428.0
68	4428.0
69	4428.0
70	3427.5
71	2427.0
72	2427.0
73	2427.0
74	1894.5
75	1362.0
76	1362.0
77	1362.0
78	1156.5
79	951.0
80	951.0
81	951.0
82	663.0
83	375.0
84	375.0
85	375.0
86	273.5
87	172.0
88	172.0
89	172.0
90	118.0
91	64.0
92	64.0
93	64.0
94	38.0
95	12.0
96	12.0
97	12.0
98	40.5
99	69.0
100	69.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004930285759362613
2	0.002958171455617568
3	0.005916342911235136
4	0.013804800126215317
5	0.025637485948685583
6	0.03549805746741081
7	0.05521920050486127
8	0.07691245784605676
9	0.08973120082039955
10	0.10945234385784999
11	0.10846628670597748
12	0.1262153154396829
13	0.11142445816159505
14	0.11339657246534009
15	0.10452205809848739
16	0.10945234385784999
17	0.12029897252844775
18	0.11635474392095765
19	0.12128502968032027
20	0.11339657246534009
21	0.11734080107283018
22	0.1360758869584081
23	0.13015954404717298
24	0.1262153154396829
25	0.12029897252844775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	101414.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.1024710592226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.29947211509531	65.8291754590096
2	4.939689833429064	7.123276865127102
3	1.166543584694073	2.523320251641785
4	0.5743825387708213	1.656576015145838
5	0.3377916358961735	1.2177805825625654
6	0.2311205929815924	0.9998619519987378
7	0.19829873362325975	1.0008480091506102
8	0.15043352205902466	0.8677302936478198
9	0.1340225923798583	0.8697024079515648
>10	0.8807198927819261	12.654071429980082
>50	0.06974645113645689	3.584317747056619
>100	0.017778507152430185	1.6733389867276707
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	185	0.18242057309641668	No Hit
GAATAGGACCGCGGTTCTATTTTGT	149	0.14692251562900585	No Hit
TCGTAGTTCCGACCATAAACGATGC	145	0.14297828702151577	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	145	0.14297828702151577	No Hit
GTATCTGATCGTCTTCGAACCTCCG	133	0.1311456011990455	No Hit
ATCAGATACCGTCGTAGTTCCGACC	132	0.13015954404717298	No Hit
GAACTACGACGGTATCTGATCGTCT	130	0.12818742974342795	No Hit
GAATAACGCCGCCGCATCGCCAGTC	121	0.11931291537657523	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	117	0.11536868676908514	No Hit
CTATTGGAGCTGGAATTACCGCGGC	115	0.11339657246534009	No Hit
CATCTAAGGGCATCACAGACCTGTT	114	0.11241051531346757	No Hit
GTATCAACGCAGAGTACTTTTTTTT	107	0.10550811525035991	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	104	0.10254994379474233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAG	35	0.0021488133	16.288675	3
GTATCAA	85	2.6101407E-7	13.414204	1
TAAGAGG	55	0.0030304121	12.093108	4
AAGAGGG	70	0.0014684362	10.859117	5
CATGGGA	95	1.6067189E-4	10.001819	4
TATCAAC	135	6.5891494E-5	8.44598	2
ATCAACG	140	1.0074246E-4	8.144338	3
TCAACGC	140	1.0074246E-4	8.144338	4
CAACGCA	145	1.5149401E-4	7.8634987	5
AACGCAG	145	1.5149401E-4	7.8634987	6
CGCAGAG	160	4.7140618E-4	7.1262956	8
CAGAGTA	175	1.6946162E-4	7.0584264	10
ACATGGG	220	7.831235E-6	6.9103475	3
ACGCAGA	165	6.702135E-4	6.910347	7
AGAGTAC	180	2.4097285E-4	6.858973	11
GCAGAGT	170	9.415639E-4	6.707102	9
GTACATG	230	1.5117987E-5	6.609897	1
TACATGG	240	2.826195E-5	6.334485	2
>>END_MODULE