FastQCFastQC Report
Thu 2 Feb 2017
SRR4062655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101414
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG3570.35202240321849054No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC2000.19721143037450453No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG1490.14692251562900585No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT1220.12029897252844775No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC1100.10846628670597748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTC301.292744E-436.6666688
CATTTAC353.197275E-431.4285721
GTTGAAG300.00572054429.3333361
AAAGTAT300.00572054429.3333366
GATATAC300.00572054429.3333361
AACTGTA300.00572054429.3333367
CAACTGT300.00572054429.3333366
GGACAGT300.00572054429.3333366
ATACACT406.987499E-427.54
TACGCTA500.002564320822.09
CGCTATT500.002564320822.011
CCCCCAA500.002564320822.024
ACGCTAT500.002564320822.010
ATACGCT500.002564320822.08
TTACCGC500.002564320822.028
TACACTG803.5617686E-622.05
CATGGGG654.8894476E-420.3076924
GTTCTAC654.8894476E-420.30769211
GGCGTCC654.8894476E-420.30769219
CATCGTT654.8894476E-420.30769228
Produced by FastQC (version 0.11.5)