FastQCFastQC Report
Thu 2 Feb 2017
SRR4062654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220290
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39711.80262381406328No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38061.7277225475509557No Hit
GTACATGGAAGCAGTGGTATCAACG28911.3123609787098824No Hit
TATCAACGCAGAGTACTTTTTTTTT27021.2265649825230378No Hit
TCCATGTACTCTGCGTTGATACCAC26871.2197557764764628No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26271.192518952290163No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18810.8538744382405011No Hit
GGTATCAACGCAGAGTACTTTTTTT14180.6436969449362204No Hit
GTACATGGGGTGGTATCAACGCAAA12970.588769349493849No Hit
GAGTACATGGAAGCAGTGGTATCAA12630.5733351491216124No Hit
CATGTACTCTGCGTTGATACCACTG12140.5510917427028008No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8950.4062826274456398No Hit
GTACATGGGTGGTATCAACGCAAAA7980.3622497616777884No Hit
GCTTCCATGTACTCTGCGTTGATAC7880.35771029098007173No Hit
GCGTTGATACCACTGCTTCCATGTA7600.3449997730264651No Hit
GTATCAACGCAGAGTACATGGGGTG7330.33274320214263015No Hit
CCCATGTACTCTGCGTTGATACCAC7230.32820373144491355No Hit
CATGGAAGCAGTGGTATCAACGCAG6940.31503926642153524No Hit
GTATCAACGCAGAGTACATGGGAAG6650.301874801398157No Hit
GTACATGGGAAGCAGTGGTATCAAC6050.2746379772118571No Hit
GTATCAACGCAGAGTACATGGAAGC5960.2705524535839121No Hit
GTACATGGGAGTGGTATCAACGCAA5950.2700985065141404No Hit
ATCAACGCAGAGTACTTTTTTTTTT5550.25194062372327386No Hit
GTATCAACGCAGAGTACATGGGCAG4960.22515774660674565No Hit
ACGCAGAGTACATGGAAGCAGTGGT4910.22288801125788732No Hit
ACTCTGCGTTGATACCACTGCTTCC4640.21063144037405238No Hit
TATCAACGCAGAGTACATGGGGTGG4380.1988288165599891No Hit
ACGCAGAGTACATGGGGTGGTATCA4300.1951972400018158No Hit
GTACATGGGCAGTGGTATCAACGCA4160.18884198102501248No Hit
CAGTGGTATCAACGCAGAGTACATG4070.1847564573970675No Hit
GTCCTAAAGTGTGTATTTCTCATTT3750.17023015116437423No Hit
TATCAACGCAGAGTACATGGAAGCA3720.16886830995505925No Hit
CTGTAGGACGTGGAATATGGCAAGA3590.1629669980480276No Hit
TATCAACGCAGAGTACATGGGAAGC3580.16251305097825594No Hit
GGTATCAACGCAGAGTACATGGGAA3520.15978936855962594No Hit
GTCCTACAGTGGACATTTCTAAATT3460.15706568614099595No Hit
GCGTTGATACCACTGCTTCCCATGT3450.1566117390712243No Hit
GGTATCAACGCAGAGTACATGGGGT3390.1538880566525943No Hit
GTATCAACGCAGAGTACATGGGTGG3190.144809115257161No Hit
GTATCAACGCAGAGTACATGGGAGT3190.144809115257161No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3160.14344727404784602No Hit
ATACCACTGCTTCCATGTACTCTGC2870.13028280902446773No Hit
GTATTAGAGGCACTGCCTGCCCAGT2870.13028280902446773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2720.12347360297789278No Hit
CTTTAGGACGTGAAATATGGCGAGG2680.12165781469880611No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2640.11984202641971947No Hit
AAAAAGTACTCTGCGTTGATACCAC2610.11848018521040447No Hit
AAGCAGTGGTATCAACGCAGAGTAC2460.11167097916382951No Hit
CAACGCAGAGTACTTTTTTTTTTTT2450.11121703209405784No Hit
TATCAACGCAGAGTACATGGGCAGT2430.1103091379545145No Hit
CCATAGGGTCTTCTCGTCTTATTAT2410.10940124381497117No Hit
CTCTAATACTTGTAATGCTAGAGGT2400.1089472967451995No Hit
GAGTACATGGGGTGGTATCAACGCA2390.10849334967542786No Hit
GGTATCAACGCAGAGTACATGGGCA2360.10713150846611286No Hit
GGTATCAACGCAGAGTACATGGAAG2350.10667756139634119No Hit
CCACTGCTTCCATGTACTCTGCGTT2340.10622361432656952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCTA250.006022232618.9965910
GTTTAAA250.00603806918.9879591
TTAGAAA651.8953506E-716.0740383
ATTTAGA651.9051913E-716.0667341
AAATGTC755.7800207E-815.1972737
TTTAGAA704.394842E-714.9258932
GAAATGT704.394842E-714.9258936
TAGAAAT704.394842E-714.9258934
AGAAATG653.346673E-614.6127625
ACATGAG400.005266331614.2474433
GATATAC602.5638405E-514.2409711
TGTCCAC903.5914127E-813.7197610
AATGTCC957.291419E-812.9976668
CCACTGT905.3168696E-712.67015313
GTAGAAC905.346301E-712.6643943
GGAGTGG1750.012.4834748
GTCCACT853.8979524E-612.29470611
TATACAC853.9078823E-612.2919123
AAATGGG701.0845315E-412.2120933
TGATACA550.00304777912.09423717
Produced by FastQC (version 0.11.5)