##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	220290
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.58331744518589	32.0	32.0	32.0	32.0	32.0
2	31.333333333333332	32.0	32.0	32.0	32.0	32.0
3	31.46813291570203	32.0	32.0	32.0	32.0	32.0
4	31.6047709837033	32.0	32.0	32.0	32.0	32.0
5	31.518003540787145	32.0	32.0	32.0	32.0	32.0
6	35.03333333333333	36.0	36.0	36.0	36.0	36.0
7	35.02991511189795	36.0	36.0	36.0	36.0	36.0
8	35.021902946116484	36.0	36.0	36.0	36.0	36.0
9	34.99008579599619	36.0	36.0	36.0	36.0	36.0
10	34.99492941123065	36.0	36.0	36.0	36.0	36.0
11	35.07560488447047	36.0	36.0	36.0	36.0	36.0
12	34.99513368741205	36.0	36.0	36.0	36.0	36.0
13	34.97944981615144	36.0	36.0	36.0	36.0	36.0
14	34.94514503608879	36.0	36.0	36.0	36.0	36.0
15	34.958495619410776	36.0	36.0	36.0	36.0	36.0
16	34.97150574243043	36.0	36.0	36.0	36.0	36.0
17	34.97987198692633	36.0	36.0	36.0	36.0	36.0
18	34.94874937582278	36.0	36.0	36.0	36.0	36.0
19	34.86188660402197	36.0	36.0	36.0	32.0	36.0
20	34.73013300649144	36.0	36.0	36.0	32.0	36.0
21	34.656688910073086	36.0	36.0	36.0	32.0	36.0
22	34.69595079213764	36.0	36.0	36.0	32.0	36.0
23	34.70819374460938	36.0	36.0	36.0	32.0	36.0
24	34.729887875073764	36.0	36.0	36.0	32.0	36.0
25	34.71041808525126	36.0	36.0	36.0	32.0	36.0
26	34.689409414862226	36.0	36.0	36.0	32.0	36.0
27	34.65731989650007	36.0	36.0	36.0	32.0	36.0
28	34.64141359117527	36.0	36.0	36.0	32.0	36.0
29	34.58113849925099	36.0	36.0	36.0	32.0	36.0
30	34.50717690317309	36.0	36.0	36.0	32.0	36.0
31	34.452054110490714	36.0	36.0	36.0	32.0	36.0
32	34.3137092015071	36.0	36.0	36.0	32.0	36.0
33	34.15571746334378	36.0	36.0	36.0	32.0	36.0
34	34.042657406146446	36.0	36.0	36.0	32.0	36.0
35	33.88228698533751	36.0	36.0	36.0	32.0	36.0
36	33.766707521902944	36.0	36.0	36.0	32.0	36.0
37	33.6832992873031	36.0	36.0	36.0	27.0	36.0
38	33.56988969086205	36.0	36.0	36.0	21.0	36.0
39	33.39700848881021	36.0	36.0	36.0	21.0	36.0
40	33.23524445049707	36.0	36.0	36.0	14.0	36.0
41	33.16920423078669	36.0	36.0	36.0	14.0	36.0
42	32.952535294384674	36.0	36.0	36.0	14.0	36.0
43	32.85681601525262	36.0	36.0	36.0	14.0	36.0
44	32.75790094874937	36.0	36.0	36.0	14.0	36.0
45	32.453570293703756	36.0	36.0	36.0	14.0	36.0
46	32.342298787961326	36.0	36.0	36.0	14.0	36.0
47	32.086436061555226	36.0	36.0	36.0	14.0	36.0
48	31.927681692314675	36.0	36.0	36.0	14.0	36.0
49	31.774683371918833	36.0	36.0	36.0	14.0	36.0
50	31.202256116936766	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	8.0
21	15.0
22	28.0
23	128.0
24	356.0
25	837.0
26	1734.0
27	3244.0
28	5683.0
29	7765.0
30	10068.0
31	12748.0
32	17044.0
33	21993.0
34	34025.0
35	104609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.579708434161596	19.923816960941437	13.507280065740787	19.989194539156173
2	12.715576922203912	23.834102805053544	41.16877987661214	22.2815403961304
3	18.836250726321907	32.439806072051134	29.135041400348634	19.58890180127833
4	10.337282672840347	17.887784284352445	37.181896590857505	34.59303645194971
5	10.086703890326387	42.07998547369377	33.08684007444732	14.746470561532526
6	26.66022053048977	37.72578499866084	20.35662567468802	15.257368796161371
7	24.39329974125017	35.46189114349267	23.150392664215353	16.994416451041808
8	28.50061282854419	31.301466249035364	21.882518498343092	18.315402424077355
9	28.813382359616867	14.515411503018749	22.23069590085796	34.44051023650642
10	16.73566662127196	28.27908665849562	32.703708747560036	22.281537972672385
11	31.403150392664216	24.683825865904037	24.459576013436834	19.453447727994917
12	24.38467474692451	27.991738163330155	31.045440101684147	16.578146988061192
13	29.85519088474284	23.025103272958372	29.152480820736304	17.967225021562484
14	23.511734531753596	22.221616959462526	30.691815334332016	23.57483317445186
15	23.407326705706115	27.838758000817105	31.63784102773616	17.116074265740615
16	20.15207226837351	28.26909982296064	31.2592491715466	20.31957873711925
17	17.514639793000136	27.445185891325067	32.68055744700168	22.35961686867311
18	19.05170457124699	24.645694312043215	37.629034454582595	18.673566662127197
19	20.45848654046938	27.54868582323301	34.43960234236688	17.553225293930726
20	21.936538199645923	23.78546461482591	37.10336374778701	17.17463343774116
21	22.868037586817376	24.70425348404376	31.76812383676063	20.65958509237823
22	21.228380770802126	27.007580916065187	32.68373507649008	19.080303236642607
23	19.379908302691906	26.926324390576056	33.69830677742976	19.995460529302285
24	19.210586045667075	28.520132552544375	33.88260928775705	18.386672114031505
25	20.91606518679922	26.54455490489809	33.51264242589314	19.02673748240955
26	17.315810976440147	27.061600617368015	35.717917290843886	19.90467111534795
27	19.65681601525262	28.91098097961778	34.59349039901947	16.83871260611013
28	18.41300104407826	26.71115347950429	34.872213899859275	20.003631576558174
29	18.035317082028236	28.329928730310044	35.670706795587634	17.964047392074082
30	19.914657950882926	26.767442916155975	35.212674202188026	18.105224930773073
31	17.835580371328703	27.55140950565164	34.50133914385582	20.11167097916383
32	17.10926505969404	29.23691497571383	34.43914839529711	19.21467156929502
33	17.70892913886241	28.638612737754777	34.61028644060102	19.042171682781788
34	17.36075173634754	29.347224113668346	35.914930319124785	17.377093830859323
35	19.39715829134323	29.09982296064279	33.305188615007495	18.19783013300649
36	18.638612737754777	29.403967497389804	33.60252394570793	18.354895819147487
37	19.61596077897317	29.616414726042944	32.259748513323345	18.507875981660536
38	18.49471151663716	30.908802033682875	31.522992419083934	19.073494030596034
39	18.052113123609786	30.17794725135049	32.932952017794726	18.836987607244996
40	19.622316037949975	30.31912479004948	31.93336057015752	18.125198601843024
41	18.094784148168326	30.100776249489307	32.96427436560897	18.840165236733398
42	19.36175041990104	31.653729175178174	31.55249897861909	17.432021426301695
43	18.013981569748967	30.620091697308094	30.990966453311543	20.374960279631395
44	17.95133687412048	30.286894548095695	32.577511462163514	19.18425711562032
45	18.802033682872576	30.607381179354487	30.867946797403423	19.722638340369514
46	18.80384947115166	29.9182895274411	30.942848063915747	20.335012937491488
47	20.31276953107268	29.799355395160926	30.284170865677062	19.603704208089336
48	18.06890916519134	33.89214217622225	27.702119932815833	20.336828725770577
49	18.23550773979754	30.53656543647011	31.337328067547325	19.890598756185028
50	17.170547914113214	34.08552362794498	27.247264968904627	21.49666348903718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	87.5
2	85.0
3	265.5
4	446.0
5	467.0
6	488.0
7	418.0
8	348.0
9	464.0
10	580.0
11	877.0
12	1174.0
13	2009.0
14	2844.0
15	3918.5
16	4993.0
17	5569.5
18	6146.0
19	6307.0
20	6468.0
21	6689.5
22	6911.0
23	6655.5
24	6400.0
25	5680.5
26	4961.0
27	5331.5
28	5702.0
29	6192.0
30	6682.0
31	7330.5
32	7979.0
33	9196.0
34	10413.0
35	11463.0
36	12513.0
37	13004.5
38	13496.0
39	12594.5
40	11693.0
41	11956.0
42	12219.0
43	11918.0
44	11617.0
45	13303.0
46	14989.0
47	15906.0
48	16823.0
49	14555.5
50	12288.0
51	10014.0
52	7740.0
53	6976.0
54	6212.0
55	5834.5
56	5457.0
57	5224.0
58	4991.0
59	4498.5
60	4006.0
61	3701.0
62	3396.0
63	3112.0
64	2828.0
65	2636.0
66	2444.0
67	2108.0
68	1772.0
69	1514.5
70	1257.0
71	1001.5
72	746.0
73	616.0
74	486.0
75	376.0
76	266.0
77	209.5
78	153.0
79	129.0
80	105.0
81	71.5
82	38.0
83	28.0
84	18.0
85	15.0
86	12.0
87	9.5
88	7.0
89	5.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014072359162921603
2	0.004085523627944981
3	9.078941395433292E-4
4	0.0
5	0.0
6	0.0013618412093149939
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	220290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.14318998421447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60220775827098	38.13620039059194
2	13.15829298487251	12.932809840470927
3	3.8359518589847723	5.65532732929168
4	1.5153450648727036	2.9787556165872435
5	0.8717655691209173	2.1420670492503047
6	0.5020839066041262	1.4804402886157901
7	0.3735677757828193	1.2850818524092864
8	0.25801005769888885	1.0143549826667706
9	0.1912660504352596	0.8459481468663279
>10	1.4006252164197517	13.316828296236002
>50	0.17333484146263353	5.890794599486428
>100	0.10659096572702174	9.51348885995344
>500	0.006973240748496751	2.125640334992942
>1k	0.003984708999141	2.682262412580914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1607	0.7294929411230651	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1408	0.6391574742385038	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1263	0.5733351491216124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.5451904307957692	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	755	0.3427300376776068	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	698	0.3168550547006219	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	634	0.2878024422352354	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	609	0.27645376549094375	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	595	0.2700985065141404	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	528	0.23968405283943894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	523	0.2374143174905806	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	485	0.22016432883925735	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	468	0.21244722865313903	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	462	0.20972354623450903	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	423	0.19201961051341412	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	403	0.18294066911798085	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	400	0.18157882790866586	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	374	0.16977620409460256	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	368	0.1670525216759726	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	360	0.16342094511779928	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	296	0.13436833265241271	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	288	0.1307367560942394	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	282	0.12801307367560943	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	280	0.1271051795360661	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	277	0.12574333832675108	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	262	0.11893413228017613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	256	0.11621044986154613	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	242	0.10985519088474284	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	237	0.1075854555358845	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	230	0.10440782604748287	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	228	0.10349993190793953	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	224	0.10168414362885289	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	224	0.10168414362885289	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	224	0.10168414362885289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.5394706977166464E-4	0.0
10	0.0	0.0	0.0	4.5394706977166464E-4	0.0
11	0.0	0.0	0.0	4.5394706977166464E-4	0.0
12	0.0	0.0	0.0	4.5394706977166464E-4	0.0
13	0.0	0.0	0.0	9.078941395433293E-4	0.0
14	0.0	0.0	0.0	9.078941395433293E-4	0.0
15	0.0	0.0	0.0	9.078941395433293E-4	0.0
16	0.0	0.0	0.0	0.0013618412093149939	0.0
17	0.0	0.0	0.0	0.0013618412093149939	0.0
18	0.0	0.0	0.0	0.0018157882790866586	0.0
19	0.0	0.0	0.0	0.0027236824186299877	0.0
20	0.0	0.0	0.0	0.0031776294884016524	0.0
21	0.0	0.0	0.0	0.003631576558173317	0.0
22	0.0	0.0	0.0	0.007263153116346634	0.0
23	0.0	0.0	0.0	0.019973671069953243	0.0
24	0.0	0.0	0.0	0.04221707748876481	0.0
25	0.0	0.0	0.0	0.0512960188841981	0.0
26	0.0	0.0	0.0	0.06400653683780472	0.0
27	0.0	0.0	0.0	0.08443415497752962	0.0
28	0.0	0.0	0.0	0.11848018521040447	0.0
29	0.0	0.0	0.0	0.17658541014117754	0.0
30	0.0	0.0	0.0	0.2578419356303055	0.0
31	0.0	0.0	0.0	0.3981115801897499	0.0
32	0.0	0.0	0.0	0.5851377729356757	0.0
33	0.0	0.0	0.0	0.7530981887511916	0.0
34	0.0	0.0	0.0	0.9383085932180307	0.0
35	0.0	0.0	0.0	1.1444005628943665	0.0
36	0.0	0.0	0.0	1.4135911752689636	0.0
37	0.0	0.0	0.0	1.8053474964819103	0.0
38	0.0	0.0	0.0	2.2738208724862683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGA	30	0.0057225204	29.346352	1
TACGGAC	30	0.00573524	29.333029	13
AAGCACT	30	0.00573524	29.333029	38
GATGATA	30	0.00573524	29.333029	44
GTACGGA	30	0.00573524	29.333029	12
ATACATG	50	8.285993E-5	26.411718	1
TAGGCAA	50	8.311645E-5	26.399727	6
TAGGACC	75	6.958726E-8	26.399727	4
GACTATA	45	0.0013909875	24.455296	1
ATAGGCA	45	0.0013946744	24.444193	5
TATAGGC	45	0.0013946744	24.444193	4
TCACACT	55	1.5879673E-4	23.999754	16
ACACTAT	55	1.5879673E-4	23.999754	18
CTAATAC	85	2.334782E-7	23.293877	3
TAATACT	95	2.6604539E-8	23.157654	4
AGGACCT	125	4.0017767E-11	22.879765	5
GTATTAG	135	3.6379788E-12	22.824942	1
AAGTACA	50	0.0025671774	22.009766	1
CTGTAGG	230	0.0	22.009766	1
CGCGGTA	90	4.0509622E-7	21.999775	43
>>END_MODULE