FastQCFastQC Report
Thu 2 Feb 2017
SRR4062653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT231.4339152119700749No Hit
CTTTAGGACGTGAAATATGGCGAGG191.1845386533665836No Hit
CTGTAGGACGTGGAATATGGCAAGA160.997506234413965No Hit
TCCATGTACTCTGCGTTGATACCAC130.8104738154613467No Hit
GTCCTAAAGTGTGTATTTCTCATTT130.8104738154613467No Hit
GTCCTACAGTGTGCATTTCTCATTT110.6857855361596009No Hit
TTGTAGAACAGTGTATATCAATGAG100.6234413965087282No Hit
GTCCTTCAGTGTGCATTTCTCATTT90.5610972568578554No Hit
GTATCAACGCAGAGTACTTTTTTTT90.5610972568578554No Hit
ATTTAGAAATGTCCACTGTAGGACG80.4987531172069825No Hit
GTGTATATCAATGAGTTACAATGAG70.4364089775561097No Hit
TATCAACGCAGAGTACTTTTTTTTT70.4364089775561097No Hit
GTACATGGAAGCAGTGGTATCAACG70.4364089775561097No Hit
CTGTAGGACCTGGAATATGGCGAGA60.3740648379052369No Hit
CATGTACTCTGCGTTGATACCACTG60.3740648379052369No Hit
CCACTGTAGGACGTGGAATATGGCA60.3740648379052369No Hit
GAGTACATGGAAGCAGTGGTATCAA50.3117206982543641No Hit
CTGAAGGACCTGGAATATGGCGAGA50.3117206982543641No Hit
GTATTTCTCATTTTCCGTGATTTTC50.3117206982543641No Hit
CCATATTCCAGGTCCTTCAGTGTGC50.3117206982543641No Hit
CTGTAGGACATGGAATATGGCAAGA50.3117206982543641No Hit
GGTATCAACGCAGAGTACTTTTTTT50.3117206982543641No Hit
CTGTAGAACATATTAGATGAGTGAG40.24937655860349126No Hit
AGTGTGTATTTCTCATTTTCCGTGA40.24937655860349126No Hit
TTTTTCAAGTCGTCAAGTGGATGTT40.24937655860349126No Hit
CATCTAAAATGTTCTACAGTGTGGT40.24937655860349126No Hit
GTAGAACATATTAGATGAGTGAGTT40.24937655860349126No Hit
CCCATGTACTCTGCGTTGATACCAC40.24937655860349126No Hit
ATACACACTTTAGGACGTGAAATAT40.24937655860349126No Hit
GAATATGGCAAGAAAACTGAAAATC40.24937655860349126No Hit
GCTTCCATGTACTCTGCGTTGATAC40.24937655860349126No Hit
GTACATGGGAAGCAGTGGTATCAAC40.24937655860349126No Hit
GCCATATTCCACGTCCTACAGTGGA40.24937655860349126No Hit
CACTGAAGGACCTGGAATATGGCGA30.18703241895261846No Hit
GTGTATATCAATGAGTTACAATGAA30.18703241895261846No Hit
ATGAGTTACAATGAAAAACATGGAA30.18703241895261846No Hit
TTTCTAAATTTTCCACCTTTTTCAG30.18703241895261846No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30.18703241895261846No Hit
CACTTTAGGACGTGAAATATGGCGA30.18703241895261846No Hit
ACTGTAGGACGTGGAATATGGCAAG30.18703241895261846No Hit
GTAGGACGTGGAATATGGCAAGAAA30.18703241895261846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG30.18703241895261846No Hit
TATTAGATGAGTGAGTTACACTGAA30.18703241895261846No Hit
GACCTGGAATATGGCGAGAAAACTG30.18703241895261846No Hit
GAGTACATGGGAAGCAGTGGTATCA30.18703241895261846No Hit
GTATCAACGCAGAGTACATGGGAAA30.18703241895261846No Hit
CCTAAAGTGTGTATTTCTCATTTTC30.18703241895261846No Hit
CGTTTTTTAGTGATTTCGTCATTTT30.18703241895261846No Hit
ACTGAAAATCACGGAAAATGAGAAA30.18703241895261846No Hit
CTGTAGGACTTGGAATATGGCAAGA30.18703241895261846No Hit
ATTCCACGTCCTACAGTGGACATTT30.18703241895261846No Hit
CTGTTCTACAAATCCCGTTTCCAAC30.18703241895261846No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30.18703241895261846No Hit
GTTCTACAATGCCGGTTTCCAACGT20.12468827930174563No Hit
CCGCTGGCCAAGCGTCTGGCGCTGA20.12468827930174563No Hit
CTTTAGGACGTGAAATATTGCGAGG20.12468827930174563No Hit
GTACATGGGAGTGGTATCAACGCAA20.12468827930174563No Hit
GCAGAGTACTTTTTTTTTTTTTTTT20.12468827930174563No Hit
GTTCTACAATGCCGGTTTCCAACAT20.12468827930174563No Hit
CTGAAAATCACGGAAAATGAGAAAT20.12468827930174563No Hit
GTCAAGTGGATGTTTCTCATTTTCC20.12468827930174563No Hit
TATCAACGCAGAGTACATGGAAGCA20.12468827930174563No Hit
TCCTAAAGTGTGTATTTCTCATTTT20.12468827930174563No Hit
TCCTACAGTGGACATTTCTAAATTT20.12468827930174563No Hit
GTATATCAATGAGTTACAATGAGAA20.12468827930174563No Hit
ATTTAGAAATTTCCACTGTAGGACG20.12468827930174563No Hit
CTTGACGACTTGAAAAATGACGAAA20.12468827930174563No Hit
GCACACTGAAGGACCTGGAATATGG20.12468827930174563No Hit
CCTTTTTCAGTTTTCCTCGCCATAT20.12468827930174563No Hit
CATCTAATCTGTTCTACAGTGTGGT20.12468827930174563No Hit
GTCCACTGTAGGACGTGGAATATGG20.12468827930174563No Hit
CAAGAAAACTGAAAATCATGGAAAA20.12468827930174563No Hit
ATTTAGAAATGTCCACTGAAGGACG20.12468827930174563No Hit
GAGAAATACACACTTTAGGACGTGA20.12468827930174563No Hit
CCCTTAGAGCCAATCCTTTTCCCGA20.12468827930174563No Hit
GGACATGGAATATGGCAAGAAAACT20.12468827930174563No Hit
CTTTAGGACGTGAAATATGGCAAGG20.12468827930174563No Hit
ACGCAGAGTACATGGAAGCAGTGGT20.12468827930174563No Hit
TTTCTAAATTTTCCACCTTTCAGTT20.12468827930174563No Hit
ATTCCAGGTCCTACAGTGTGCATTT20.12468827930174563No Hit
GTCCTAAAGTCTGTATTTCTCATTT20.12468827930174563No Hit
GTGTGCATTTCTCATTTTTCACGTT20.12468827930174563No Hit
CGTATGTGTTTTTCAGTGTAACTCA20.12468827930174563No Hit
GTAGGACATGGAATATGGCAAGAAA20.12468827930174563No Hit
TCTAATATGTTCTACAGTGTGGTTT20.12468827930174563No Hit
GTGGTATCAACGCAGAGTACATGGG20.12468827930174563No Hit
GATATACACTGTTCTACAATGCCGG20.12468827930174563No Hit
AAAAAGTACTCTGCGTTGATACCAC20.12468827930174563No Hit
GCGTTGATACCACTGCTTCCCATGT20.12468827930174563No Hit
TTGATATACACTGTTCTACAAATCC20.12468827930174563No Hit
GAAATGCACACTGTAGGACCTGGAA20.12468827930174563No Hit
GTGTATTTCTCATTTTCCGTGATTT20.12468827930174563No Hit
GTGTGATTATTGCCTATAGTCTGAT20.12468827930174563No Hit
GTGTGGTTTTTATCATTTTCCATGT20.12468827930174563No Hit
CTACAGTGGACATTTCTAAATTTTC20.12468827930174563No Hit
TATCAACGCAGAGTACATGGGGTGG20.12468827930174563No Hit
GTGGAATATGGCAAGAAAACTGAAA20.12468827930174563No Hit
ATCCACTTGACGACTTGAAAAATGA20.12468827930174563No Hit
GTTGATACCACTGCTTCCATGTACT20.12468827930174563No Hit
TATCAACGCAGAGTACATGGGTGAG20.12468827930174563No Hit
CATCTAATATGTTCTACAGTGTGGT20.12468827930174563No Hit
GTTTTTCAGTGTAACTCACTCATCT20.12468827930174563No Hit
CGTAGGACTTTAATCGTTGAACAAA20.12468827930174563No Hit
ACAGTGGACATTTCTAAATATTCCA20.12468827930174563No Hit
GTACTCTGCGTTGATACCACTGCTT20.12468827930174563No Hit
ACACTGTTCTACAAATCCCGTTTCC20.12468827930174563No Hit
CTGTAGGACCTGGAATATGGCAAGA20.12468827930174563No Hit
GATATACACTGTTCTACAAATCCCG20.12468827930174563No Hit
CTGAAAAAGGTGGATAATTTAGAAA20.12468827930174563No Hit
GATTTTCAGTTTTCTTGCCATATTC20.12468827930174563No Hit
GCGTTGATACCACTGCTTCCATGTA20.12468827930174563No Hit
ACATCCACTTGACGACTTGAAAAAT20.12468827930174563No Hit
GCCATATTCCAGGTCCTACAGTGTG20.12468827930174563No Hit
GTATCAACGCAGAGTACATGGAATC20.12468827930174563No Hit
GTTCTACAGTGTGGTTTTTATCATT20.12468827930174563No Hit
AGTGTGTATTTCTCATTTTCCATGA20.12468827930174563No Hit
GATTAGATGAGTGAGTTACACTGAA20.12468827930174563No Hit
ACGTCCTACAGTGGACATTTCTAAA20.12468827930174563No Hit
ATGTCCTACAGTGGACATTTCTAAA20.12468827930174563No Hit
GTTCTACAAATCCCGTTTCCAACGA20.12468827930174563No Hit
GGTCCTTCAGTGTGCATTTCTCATT20.12468827930174563No Hit
CTCCTGAACAGGCATGTGCCACTGC20.12468827930174563No Hit
GATTTCGTCATTTTTCAAGTCGTCA20.12468827930174563No Hit
CTTTAGGACATGAAATATGGCGAGG20.12468827930174563No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)