##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062653_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1604
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.955112219451372	32.0	32.0	32.0	14.0	32.0
2	26.9856608478803	32.0	21.0	32.0	14.0	32.0
3	27.756857855361595	32.0	21.0	32.0	14.0	32.0
4	27.19638403990025	32.0	21.0	32.0	14.0	32.0
5	27.045511221945137	32.0	21.0	32.0	14.0	32.0
6	30.645261845386532	36.0	21.0	36.0	14.0	36.0
7	30.653990024937656	36.0	21.0	36.0	14.0	36.0
8	30.048004987531172	36.0	21.0	36.0	14.0	36.0
9	31.100997506234414	36.0	32.0	36.0	14.0	36.0
10	29.771820448877804	36.0	21.0	36.0	14.0	36.0
11	31.100374064837904	36.0	32.0	36.0	14.0	36.0
12	30.556109725685786	36.0	21.0	36.0	14.0	36.0
13	30.968204488778056	36.0	32.0	36.0	14.0	36.0
14	30.102244389027433	36.0	21.0	36.0	14.0	36.0
15	29.64650872817955	36.0	21.0	36.0	14.0	36.0
16	30.104738154613468	36.0	21.0	36.0	14.0	36.0
17	29.618453865336658	36.0	21.0	36.0	14.0	36.0
18	29.698877805486283	36.0	21.0	36.0	14.0	36.0
19	30.19139650872818	36.0	21.0	36.0	14.0	36.0
20	29.5642144638404	36.0	21.0	36.0	14.0	36.0
21	30.153366583541146	36.0	21.0	36.0	14.0	36.0
22	29.30361596009975	36.0	21.0	36.0	14.0	36.0
23	29.35785536159601	36.0	21.0	36.0	14.0	36.0
24	29.61346633416459	36.0	21.0	36.0	14.0	36.0
25	28.90586034912718	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	4.0
16	15.0
17	30.0
18	21.0
19	44.0
20	58.0
21	69.0
22	51.0
23	47.0
24	60.0
25	46.0
26	61.0
27	49.0
28	69.0
29	58.0
30	53.0
31	83.0
32	99.0
33	108.0
34	246.0
35	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.80224578914535	20.898315658140987	13.599500935745477	26.699937616968185
2	9.482220835932626	20.83593262632564	49.656893325015595	20.02495321272614
3	19.65065502183406	25.389893948845916	33.000623830318155	21.95882719900187
4	9.102244389027431	18.017456359102244	41.33416458852868	31.546134663341647
5	8.915211970074813	42.33167082294264	38.90274314214464	9.850374064837904
6	29.67581047381546	40.14962593516209	17.70573566084788	12.468827930174564
7	23.705552089831567	32.50155957579538	23.892701185277605	19.900187149095448
8	20.212102308172177	46.41297567061759	20.212102308172177	13.162819713038054
9	25.280898876404496	15.168539325842698	20.536828963795255	39.013732833957555
10	15.917602996254681	29.213483146067414	38.57677902621723	16.292134831460675
11	31.897627965043696	20.037453183520597	30.274656679151065	17.790262172284642
12	25.905118601747816	22.15980024968789	37.20349563046192	14.731585518102372
13	34.20724094881398	22.284644194756552	25.905118601747816	17.60299625468165
14	18.476903870162296	27.403245942571786	26.903870162297128	27.21598002496879
15	18.476903870162296	41.69787765293383	22.596754057428214	17.228464419475657
16	14.04494382022472	28.214731585518106	40.01248439450686	17.72784019975031
17	16.729088639201	31.210986267166042	35.51810237203495	16.541822721598002
18	16.864459712679576	25.79637726420987	42.28607120549657	15.053091817613993
19	25.109306683322924	24.48469706433479	26.483447845096812	23.922548407245472
20	22.47191011235955	30.774032459425715	32.08489388264669	14.66916354556804
21	18.925671455340414	28.419737663960028	25.546533416614615	27.108057464084943
22	20.924422236102437	35.54028732042474	28.7945034353529	14.740787008119923
23	18.738288569643974	33.22923173016864	31.105559025608994	16.926920674578387
24	21.861336664584634	29.981261711430356	32.667083073079326	15.490318550905686
25	20.04996876951905	31.105559025608994	32.72954403497813	16.114928169893815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	1.5
7	1.0
8	1.0
9	1.0
10	4.5
11	8.0
12	8.0
13	8.0
14	9.0
15	10.0
16	10.0
17	10.0
18	13.0
19	16.0
20	16.0
21	16.0
22	30.0
23	44.0
24	44.0
25	44.0
26	93.0
27	142.0
28	142.0
29	142.0
30	195.0
31	248.0
32	248.0
33	248.0
34	254.0
35	260.0
36	260.0
37	260.0
38	226.5
39	193.0
40	193.0
41	193.0
42	195.0
43	197.0
44	197.0
45	197.0
46	215.5
47	234.0
48	234.0
49	234.0
50	185.0
51	136.0
52	136.0
53	136.0
54	86.5
55	37.0
56	37.0
57	37.0
58	35.0
59	33.0
60	33.0
61	33.0
62	26.5
63	20.0
64	20.0
65	20.0
66	14.5
67	9.0
68	9.0
69	9.0
70	7.5
71	6.0
72	6.0
73	6.0
74	5.0
75	4.0
76	4.0
77	4.0
78	2.0
79	0.0
80	0.0
81	0.0
82	0.5
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.062344139650872814
2	0.062344139650872814
3	0.062344139650872814
4	0.0
5	0.0
6	0.0
7	0.062344139650872814
8	0.062344139650872814
9	0.12468827930174563
10	0.12468827930174563
11	0.12468827930174563
12	0.12468827930174563
13	0.12468827930174563
14	0.12468827930174563
15	0.12468827930174563
16	0.12468827930174563
17	0.12468827930174563
18	0.18703241895261846
19	0.18703241895261846
20	0.12468827930174563
21	0.18703241895261846
22	0.18703241895261846
23	0.18703241895261846
24	0.18703241895261846
25	0.18703241895261846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1604.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.92518703241896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.32761310452419	72.19451371571073
2	5.538221528861154	8.85286783042394
3	1.5600624024960998	3.7406483790523692
4	0.858034321372855	2.7431421446384037
5	0.46801872074883	1.8703241895261846
6	0.234009360374415	1.1221945137157108
7	0.234009360374415	1.3092269326683292
8	0.078003120124805	0.4987531172069825
9	0.15600624024961	1.1221945137157108
>10	0.5460218408736349	6.546134663341646
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	23	1.4339152119700749	No Hit
CTTTAGGACGTGAAATATGGCGAGG	19	1.1845386533665836	No Hit
CTGTAGGACGTGGAATATGGCAAGA	16	0.997506234413965	No Hit
TCCATGTACTCTGCGTTGATACCAC	13	0.8104738154613467	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	13	0.8104738154613467	No Hit
GTCCTACAGTGTGCATTTCTCATTT	11	0.6857855361596009	No Hit
TTGTAGAACAGTGTATATCAATGAG	10	0.6234413965087282	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	9	0.5610972568578554	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9	0.5610972568578554	No Hit
ATTTAGAAATGTCCACTGTAGGACG	8	0.4987531172069825	No Hit
GTGTATATCAATGAGTTACAATGAG	7	0.4364089775561097	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7	0.4364089775561097	No Hit
GTACATGGAAGCAGTGGTATCAACG	7	0.4364089775561097	No Hit
CTGTAGGACCTGGAATATGGCGAGA	6	0.3740648379052369	No Hit
CATGTACTCTGCGTTGATACCACTG	6	0.3740648379052369	No Hit
CCACTGTAGGACGTGGAATATGGCA	6	0.3740648379052369	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5	0.3117206982543641	No Hit
CTGAAGGACCTGGAATATGGCGAGA	5	0.3117206982543641	No Hit
GTATTTCTCATTTTCCGTGATTTTC	5	0.3117206982543641	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	5	0.3117206982543641	No Hit
CTGTAGGACATGGAATATGGCAAGA	5	0.3117206982543641	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.3117206982543641	No Hit
CTGTAGAACATATTAGATGAGTGAG	4	0.24937655860349126	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	4	0.24937655860349126	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	4	0.24937655860349126	No Hit
CATCTAAAATGTTCTACAGTGTGGT	4	0.24937655860349126	No Hit
GTAGAACATATTAGATGAGTGAGTT	4	0.24937655860349126	No Hit
CCCATGTACTCTGCGTTGATACCAC	4	0.24937655860349126	No Hit
ATACACACTTTAGGACGTGAAATAT	4	0.24937655860349126	No Hit
GAATATGGCAAGAAAACTGAAAATC	4	0.24937655860349126	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4	0.24937655860349126	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4	0.24937655860349126	No Hit
GCCATATTCCACGTCCTACAGTGGA	4	0.24937655860349126	No Hit
CACTGAAGGACCTGGAATATGGCGA	3	0.18703241895261846	No Hit
GTGTATATCAATGAGTTACAATGAA	3	0.18703241895261846	No Hit
ATGAGTTACAATGAAAAACATGGAA	3	0.18703241895261846	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3	0.18703241895261846	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3	0.18703241895261846	No Hit
CACTTTAGGACGTGAAATATGGCGA	3	0.18703241895261846	No Hit
ACTGTAGGACGTGGAATATGGCAAG	3	0.18703241895261846	No Hit
GTAGGACGTGGAATATGGCAAGAAA	3	0.18703241895261846	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	3	0.18703241895261846	No Hit
TATTAGATGAGTGAGTTACACTGAA	3	0.18703241895261846	No Hit
GACCTGGAATATGGCGAGAAAACTG	3	0.18703241895261846	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3	0.18703241895261846	No Hit
GTATCAACGCAGAGTACATGGGAAA	3	0.18703241895261846	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	3	0.18703241895261846	No Hit
CGTTTTTTAGTGATTTCGTCATTTT	3	0.18703241895261846	No Hit
ACTGAAAATCACGGAAAATGAGAAA	3	0.18703241895261846	No Hit
CTGTAGGACTTGGAATATGGCAAGA	3	0.18703241895261846	No Hit
ATTCCACGTCCTACAGTGGACATTT	3	0.18703241895261846	No Hit
CTGTTCTACAAATCCCGTTTCCAAC	3	0.18703241895261846	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3	0.18703241895261846	No Hit
GTTCTACAATGCCGGTTTCCAACGT	2	0.12468827930174563	No Hit
CCGCTGGCCAAGCGTCTGGCGCTGA	2	0.12468827930174563	No Hit
CTTTAGGACGTGAAATATTGCGAGG	2	0.12468827930174563	No Hit
GTACATGGGAGTGGTATCAACGCAA	2	0.12468827930174563	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2	0.12468827930174563	No Hit
GTTCTACAATGCCGGTTTCCAACAT	2	0.12468827930174563	No Hit
CTGAAAATCACGGAAAATGAGAAAT	2	0.12468827930174563	No Hit
GTCAAGTGGATGTTTCTCATTTTCC	2	0.12468827930174563	No Hit
TATCAACGCAGAGTACATGGAAGCA	2	0.12468827930174563	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	2	0.12468827930174563	No Hit
TCCTACAGTGGACATTTCTAAATTT	2	0.12468827930174563	No Hit
GTATATCAATGAGTTACAATGAGAA	2	0.12468827930174563	No Hit
ATTTAGAAATTTCCACTGTAGGACG	2	0.12468827930174563	No Hit
CTTGACGACTTGAAAAATGACGAAA	2	0.12468827930174563	No Hit
GCACACTGAAGGACCTGGAATATGG	2	0.12468827930174563	No Hit
CCTTTTTCAGTTTTCCTCGCCATAT	2	0.12468827930174563	No Hit
CATCTAATCTGTTCTACAGTGTGGT	2	0.12468827930174563	No Hit
GTCCACTGTAGGACGTGGAATATGG	2	0.12468827930174563	No Hit
CAAGAAAACTGAAAATCATGGAAAA	2	0.12468827930174563	No Hit
ATTTAGAAATGTCCACTGAAGGACG	2	0.12468827930174563	No Hit
GAGAAATACACACTTTAGGACGTGA	2	0.12468827930174563	No Hit
CCCTTAGAGCCAATCCTTTTCCCGA	2	0.12468827930174563	No Hit
GGACATGGAATATGGCAAGAAAACT	2	0.12468827930174563	No Hit
CTTTAGGACGTGAAATATGGCAAGG	2	0.12468827930174563	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2	0.12468827930174563	No Hit
TTTCTAAATTTTCCACCTTTCAGTT	2	0.12468827930174563	No Hit
ATTCCAGGTCCTACAGTGTGCATTT	2	0.12468827930174563	No Hit
GTCCTAAAGTCTGTATTTCTCATTT	2	0.12468827930174563	No Hit
GTGTGCATTTCTCATTTTTCACGTT	2	0.12468827930174563	No Hit
CGTATGTGTTTTTCAGTGTAACTCA	2	0.12468827930174563	No Hit
GTAGGACATGGAATATGGCAAGAAA	2	0.12468827930174563	No Hit
TCTAATATGTTCTACAGTGTGGTTT	2	0.12468827930174563	No Hit
GTGGTATCAACGCAGAGTACATGGG	2	0.12468827930174563	No Hit
GATATACACTGTTCTACAATGCCGG	2	0.12468827930174563	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2	0.12468827930174563	No Hit
GCGTTGATACCACTGCTTCCCATGT	2	0.12468827930174563	No Hit
TTGATATACACTGTTCTACAAATCC	2	0.12468827930174563	No Hit
GAAATGCACACTGTAGGACCTGGAA	2	0.12468827930174563	No Hit
GTGTATTTCTCATTTTCCGTGATTT	2	0.12468827930174563	No Hit
GTGTGATTATTGCCTATAGTCTGAT	2	0.12468827930174563	No Hit
GTGTGGTTTTTATCATTTTCCATGT	2	0.12468827930174563	No Hit
CTACAGTGGACATTTCTAAATTTTC	2	0.12468827930174563	No Hit
TATCAACGCAGAGTACATGGGGTGG	2	0.12468827930174563	No Hit
GTGGAATATGGCAAGAAAACTGAAA	2	0.12468827930174563	No Hit
ATCCACTTGACGACTTGAAAAATGA	2	0.12468827930174563	No Hit
GTTGATACCACTGCTTCCATGTACT	2	0.12468827930174563	No Hit
TATCAACGCAGAGTACATGGGTGAG	2	0.12468827930174563	No Hit
CATCTAATATGTTCTACAGTGTGGT	2	0.12468827930174563	No Hit
GTTTTTCAGTGTAACTCACTCATCT	2	0.12468827930174563	No Hit
CGTAGGACTTTAATCGTTGAACAAA	2	0.12468827930174563	No Hit
ACAGTGGACATTTCTAAATATTCCA	2	0.12468827930174563	No Hit
GTACTCTGCGTTGATACCACTGCTT	2	0.12468827930174563	No Hit
ACACTGTTCTACAAATCCCGTTTCC	2	0.12468827930174563	No Hit
CTGTAGGACCTGGAATATGGCAAGA	2	0.12468827930174563	No Hit
GATATACACTGTTCTACAAATCCCG	2	0.12468827930174563	No Hit
CTGAAAAAGGTGGATAATTTAGAAA	2	0.12468827930174563	No Hit
GATTTTCAGTTTTCTTGCCATATTC	2	0.12468827930174563	No Hit
GCGTTGATACCACTGCTTCCATGTA	2	0.12468827930174563	No Hit
ACATCCACTTGACGACTTGAAAAAT	2	0.12468827930174563	No Hit
GCCATATTCCAGGTCCTACAGTGTG	2	0.12468827930174563	No Hit
GTATCAACGCAGAGTACATGGAATC	2	0.12468827930174563	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2	0.12468827930174563	No Hit
AGTGTGTATTTCTCATTTTCCATGA	2	0.12468827930174563	No Hit
GATTAGATGAGTGAGTTACACTGAA	2	0.12468827930174563	No Hit
ACGTCCTACAGTGGACATTTCTAAA	2	0.12468827930174563	No Hit
ATGTCCTACAGTGGACATTTCTAAA	2	0.12468827930174563	No Hit
GTTCTACAAATCCCGTTTCCAACGA	2	0.12468827930174563	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	2	0.12468827930174563	No Hit
CTCCTGAACAGGCATGTGCCACTGC	2	0.12468827930174563	No Hit
GATTTCGTCATTTTTCAAGTCGTCA	2	0.12468827930174563	No Hit
CTTTAGGACATGAAATATGGCGAGG	2	0.12468827930174563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE