FastQCFastQC Report
Thu 2 Feb 2017
SRR4062653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100.6234413965087282No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA80.4987531172069825No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA70.4364089775561097No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA60.3740648379052369No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC60.3740648379052369No Hit
GTAGAACATATTAGATGAGTGAGTTACACTGAAAAACACATTCGTTGGAA60.3740648379052369No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA50.3117206982543641No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.24937655860349126No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC40.24937655860349126No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.24937655860349126No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.18703241895261846No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTCATTGTAACTCAT30.18703241895261846No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG30.18703241895261846No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG30.18703241895261846No Hit
CTCATTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCAGGTCCTTCAGT30.18703241895261846No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA30.18703241895261846No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT30.18703241895261846No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC30.18703241895261846No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT30.18703241895261846No Hit
ATATAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA30.18703241895261846No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT30.18703241895261846No Hit
CATTGTAACTCATTGATATACACTGTTCTACAAATCCCGTTTCCAACGAA20.12468827930174563No Hit
GAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATG20.12468827930174563No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTG20.12468827930174563No Hit
TATTAGATGTGTGAGTTACACTGAAAAACACATTCGTTGGAAACGGGATT20.12468827930174563No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.12468827930174563No Hit
GCTTGCGCCACTGCATTCGAGCCTGGGCGACAGAGCGAGACTCTGTCTCA20.12468827930174563No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA20.12468827930174563No Hit
CACTCATCTAATATGTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTT20.12468827930174563No Hit
TATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGA20.12468827930174563No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC20.12468827930174563No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA20.12468827930174563No Hit
ATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTT20.12468827930174563No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCATTG20.12468827930174563No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATAT20.12468827930174563No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.12468827930174563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT20.12468827930174563No Hit
GTCCTACAGTGGACATTTCTAAATTATCCACCTTTTTCAGCTGTCTCTTA20.12468827930174563No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.12468827930174563No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20.12468827930174563No Hit
CTGTAGAACAGATTAGATGAGTGAGTTACACTGAAAAACACATTCGTTGG20.12468827930174563No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG20.12468827930174563No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12468827930174563No Hit
GTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTC20.12468827930174563No Hit
ATTTAGAAATGTCCACTGTAGGACATGGAATATGGCAAGAAAACTGAAAA20.12468827930174563No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATT20.12468827930174563No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAATATTTA20.12468827930174563No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA20.12468827930174563No Hit
TATTCCACGTCCTACAGTGGACTTTTCTAAATTTTCCACCTTTTTCAGTT20.12468827930174563No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATCGAAAATGA20.12468827930174563No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.12468827930174563No Hit
CTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCAC20.12468827930174563No Hit
CTAAAAAACGTGAAAAATGAGAAATGCACACTGAAGGACCTGGAATATGG20.12468827930174563No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA20.12468827930174563No Hit
CTCATTTTTCACGTTTTTTAGTGGTTTCGTCATTTTTCAAGTCGTCAAGT20.12468827930174563No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATGAAAACCAC20.12468827930174563No Hit
GTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCATTTTTCAA20.12468827930174563No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC20.12468827930174563No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAA20.12468827930174563No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT20.12468827930174563No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20.12468827930174563No Hit
CAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTG20.12468827930174563No Hit
CACATTAAAACGTGAAAAATGAGAAATACACACTGAAGGACCTGGAATAT20.12468827930174563No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.12468827930174563No Hit
GGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTT20.12468827930174563No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC20.12468827930174563No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)