FastQCFastQC Report
Thu 2 Feb 2017
SRR4062652_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062652_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851037
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG60180.3251150571274372No Hit
TCCATGTACTCTGCGTTGATACCAC51330.2773040193145788No Hit
GTATCAACGCAGAGTACTTTTTTTT37410.2021029293309642No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27690.14959182339412988No Hit
TATCAACGCAGAGTACTTTTTTTTT27400.14802513401947123No Hit
GAGTACATGGAAGCAGTGGTATCAA26740.14445956509783434No Hit
CATGTACTCTGCGTTGATACCACTG24420.13192605010056527No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23880.1290087664374078No Hit
GCGCAAGACGGACCAGAGCGAAAGC23830.12873864757970804No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23120.12490295980037136No Hit
GAATAGGACCGCGGTTCTATTTTGT22380.12090520070641483No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21490.11609708503935903No Hit
GGGTAGGCACACGCTGAGCCAGTCA20860.11269358743234198No Hit
GAACTACGACGGTATCTGATCGTCT20320.10977630376918451No Hit
GAATAACGCCGCCGCATCGCCAGTC20030.10820961439452588No Hit
GATTAAGAGGGACGGCCGGGGGCAT19310.10431990284364925No Hit
GTATCTGATCGTCTTCGAACCTCCG19220.1038336888997897No Hit
CTATTGGAGCTGGAATTACCGCGGC19190.10367161758516982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAC602.5675385E-514.250730511
AGTACGT500.001501296313.2981635
ACGAACG1251.0186341E-1012.92101215
CGGTTCT3900.012.66868712
CGAACGA1405.2750693E-1112.21590316
TCGACAT550.00306702612.09185513
TCGCGTA2000.011.875939
TAGAACC2600.011.6900614
GTTATAG2050.011.5790681
ACCGTGT1157.068047E-811.5661248
GTTTAGA1406.85759E-1011.5294461
AAGACGG4400.011.4416015
TGTAGGA6400.011.4250192
CGGTCCA5000.011.40089210
ACGGTAT3500.011.4008929
AGAACCG2250.011.3984265
CAAGACG4700.011.3169744
TCCATTA4300.011.2683248
GTCCTAC5900.011.265061
GACAGCG855.3196476E-511.1773457
Produced by FastQC (version 0.11.5)