##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062652_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1851037
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.142515249560113	32.0	32.0	32.0	32.0	32.0
2	30.813217131802336	32.0	32.0	32.0	32.0	32.0
3	30.79972037295851	32.0	32.0	32.0	32.0	32.0
4	30.864917340928354	32.0	32.0	32.0	32.0	32.0
5	30.641919637478882	32.0	32.0	32.0	32.0	32.0
6	34.297473254181305	36.0	36.0	36.0	32.0	36.0
7	34.16739589754284	36.0	36.0	36.0	32.0	36.0
8	34.16553423837557	36.0	36.0	36.0	32.0	36.0
9	34.33340284391938	36.0	36.0	36.0	32.0	36.0
10	33.99448309245034	36.0	36.0	36.0	32.0	36.0
11	34.333063034396396	36.0	36.0	36.0	32.0	36.0
12	34.088523892283085	36.0	36.0	36.0	32.0	36.0
13	34.2203710676772	36.0	36.0	36.0	32.0	36.0
14	34.1325446222847	36.0	36.0	36.0	32.0	36.0
15	34.059678979944756	36.0	36.0	36.0	32.0	36.0
16	34.062196487698515	36.0	36.0	36.0	32.0	36.0
17	33.9836416019777	36.0	36.0	36.0	32.0	36.0
18	33.99880661488668	36.0	36.0	36.0	32.0	36.0
19	33.98409324070778	36.0	36.0	36.0	32.0	36.0
20	33.974211212417686	36.0	36.0	36.0	32.0	36.0
21	33.9403193993421	36.0	36.0	36.0	32.0	36.0
22	33.88751278337494	36.0	36.0	36.0	32.0	36.0
23	33.85333032240847	36.0	36.0	36.0	32.0	36.0
24	33.81703877340107	36.0	36.0	36.0	32.0	36.0
25	33.34870129554407	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	71.0
5	152.0
6	327.0
7	78.0
8	290.0
9	324.0
10	222.0
11	59.0
12	139.0
13	101.0
14	337.0
15	423.0
16	683.0
17	871.0
18	1178.0
19	1719.0
20	2652.0
21	3701.0
22	5616.0
23	7982.0
24	11287.0
25	15786.0
26	21950.0
27	28415.0
28	38661.0
29	52468.0
30	68151.0
31	94030.0
32	136119.0
33	196656.0
34	426029.0
35	734548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.893858400787515	17.259950099573935	10.959237900119186	25.886953599519362
2	16.616108054783744	19.99219875666334	37.13408035820889	26.25761283034403
3	19.449928091291106	23.898813479290183	27.989397922589276	28.661860506829434
4	12.545385975301384	14.974994488845855	35.641714466023195	36.83790506982956
5	14.508654619895491	36.57513865928376	33.526769091422985	15.389437629397767
6	34.581762325207336	34.889135830227346	16.880788588427578	13.648313256137742
7	30.367081141890868	30.20836205251461	20.859396835024587	18.565159970569933
8	28.27529487846368	32.69779594096699	19.738932392346513	19.287976788222814
9	27.17553825729821	14.3559974151753	18.793892675699134	39.67457165182736
10	15.904279658129273	26.42537181848962	31.588534934008912	26.081813589372192
11	37.570319712993346	21.09372337911067	21.982154572748755	19.353802335147225
12	24.463159694067155	23.730675669316	28.817926249607144	22.9882383870097
13	29.512539700397223	19.282601922927142	25.540336468979604	25.664521907696034
14	23.458068229084688	19.7823346049683	24.908197258655747	31.851399907291267
15	25.197081634198316	27.401918895823645	22.578502742052375	24.822496727925667
16	25.78847684812613	25.67111662037157	24.28853742114691	24.25186911035539
17	23.83387683991266	25.95806894970081	25.764267250541838	24.443786959844697
18	25.064287375657884	24.47463934701119	26.925781482423066	23.535291794907856
19	25.28814036074681	25.47562700651551	25.293549669626458	23.942682963111217
20	25.294714210376863	24.206293186212864	25.677173339823945	24.82181926358633
21	26.316270611227736	24.53089730073582	24.351581039320298	24.801251048716143
22	25.270925512932468	24.868824981307085	25.061651447226467	24.798598058533976
23	23.65117079219169	24.582740958575965	25.656535164880346	26.109553084352
24	24.190265168378865	25.499256463949184	25.495415717524462	24.81506265014749
25	24.67906553056694	24.70124370897037	25.337162404850204	25.282528355612488
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	269.0
1	269.0
2	673.0
3	1077.0
4	1077.0
5	1077.0
6	2851.5
7	4626.0
8	4626.0
9	4626.0
10	4801.5
11	4977.0
12	4977.0
13	4977.0
14	5221.5
15	5466.0
16	5466.0
17	5466.0
18	8888.0
19	12310.0
20	12310.0
21	12310.0
22	19686.5
23	27063.0
24	27063.0
25	27063.0
26	40524.5
27	53986.0
28	53986.0
29	53986.0
30	68036.5
31	82087.0
32	82087.0
33	82087.0
34	100361.0
35	118635.0
36	118635.0
37	118635.0
38	133558.0
39	148481.0
40	148481.0
41	148481.0
42	169893.0
43	191305.0
44	191305.0
45	191305.0
46	224606.5
47	257908.0
48	257908.0
49	257908.0
50	250890.5
51	243873.0
52	243873.0
53	243873.0
54	222978.0
55	202083.0
56	202083.0
57	202083.0
58	186327.0
59	170571.0
60	170571.0
61	170571.0
62	152036.0
63	133501.0
64	133501.0
65	133501.0
66	111826.0
67	90151.0
68	90151.0
69	90151.0
70	68344.0
71	46537.0
72	46537.0
73	46537.0
74	36583.0
75	26629.0
76	26629.0
77	26629.0
78	21924.5
79	17220.0
80	17220.0
81	17220.0
82	12045.0
83	6870.0
84	6870.0
85	6870.0
86	5041.5
87	3213.0
88	3213.0
89	3213.0
90	2181.5
91	1150.0
92	1150.0
93	1150.0
94	677.5
95	205.0
96	205.0
97	205.0
98	524.5
99	844.0
100	844.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008157589502532904
2	0.0027011885769976504
3	0.004267877951656287
4	0.013235824027288488
5	0.03446716624249002
6	0.0456500869512603
7	0.06634119145106229
8	0.08811277138166336
9	0.09627036088419626
10	0.11620513258243893
11	0.11669134652629849
12	0.1294409566097274
13	0.11825803590095714
14	0.12079715316333493
15	0.11112689805768333
16	0.10961423245456464
17	0.12101324824949475
18	0.12463284094267159
19	0.12814438609276854
20	0.11950058264637606
21	0.12684781557580968
22	0.1481872053340911
23	0.1374904985691804
24	0.13170995501440544
25	0.1281984098643085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1851037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.93295551124199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.95422346330868	35.647561906943984
2	13.818201675098479	12.97058088925533
3	4.446144541445362	6.2601211188061985
4	1.9684731776905944	3.695450562945033
5	0.9906523769898262	2.3247121968184823
6	0.5911046775926495	1.664537372156572
7	0.3799543176216991	1.248266535967046
8	0.27828950445461825	1.0448759145451343
9	0.20311292099038022	0.8579420716139933
>10	1.1461930872287052	9.861469395457267
>50	0.10235500363025798	3.3711527969149073
>100	0.0979831092891566	9.959996441818983
>500	0.016156931942352307	5.234114450955361
>1k	0.006924399403865274	5.255238315351
>5k	2.3081331346217577E-4	0.6039800304506983
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6018	0.3251150571274372	No Hit
TCCATGTACTCTGCGTTGATACCAC	5133	0.2773040193145788	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3741	0.2021029293309642	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2769	0.14959182339412988	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2740	0.14802513401947123	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2674	0.14445956509783434	No Hit
CATGTACTCTGCGTTGATACCACTG	2442	0.13192605010056527	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2388	0.1290087664374078	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2383	0.12873864757970804	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2312	0.12490295980037136	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2238	0.12090520070641483	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2149	0.11609708503935903	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2086	0.11269358743234198	No Hit
GAACTACGACGGTATCTGATCGTCT	2032	0.10977630376918451	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2003	0.10820961439452588	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1931	0.10431990284364925	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1922	0.1038336888997897	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1919	0.10367161758516982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.402377153995301E-5	0.0
12	0.0	0.0	0.0	5.402377153995301E-5	0.0
13	0.0	0.0	0.0	5.402377153995301E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGAC	60	2.5675385E-5	14.2507305	11
AGTACGT	50	0.0015012963	13.298163	5
ACGAACG	125	1.0186341E-10	12.921012	15
CGGTTCT	390	0.0	12.668687	12
CGAACGA	140	5.2750693E-11	12.215903	16
TCGACAT	55	0.003067026	12.091855	13
TCGCGTA	200	0.0	11.87593	9
TAGAACC	260	0.0	11.690061	4
GTTATAG	205	0.0	11.579068	1
ACCGTGT	115	7.068047E-8	11.566124	8
GTTTAGA	140	6.85759E-10	11.529446	1
AAGACGG	440	0.0	11.441601	5
TGTAGGA	640	0.0	11.425019	2
CGGTCCA	500	0.0	11.400892	10
ACGGTAT	350	0.0	11.400892	9
AGAACCG	225	0.0	11.398426	5
CAAGACG	470	0.0	11.316974	4
TCCATTA	430	0.0	11.268324	8
GTCCTAC	590	0.0	11.26506	1
GACAGCG	85	5.3196476E-5	11.177345	7
>>END_MODULE