FastQCFastQC Report
Thu 2 Feb 2017
SRR4062652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851037
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25070.13543759525066218No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24820.13408700096216336No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23480.12684781557580968No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23320.12598343523117042No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22300.1204730105340952No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20880.11280163497542188No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20410.11026251771304409No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19890.10745328159296655No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19820.10707511519218688No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC18850.10183480935281142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG602.86901E-422.0051751
TAGGACG6300.021.3019924
CGCAATA7500.021.11983136
TCTAGCG7650.019.8429828
CTAGCGG7650.019.8429829
TAGCGGC7750.019.5869430
CGGTCCA7750.019.5869410
GTATAAT2050.019.3216171
GCCGGTC8200.019.316928
GTCCTAC7300.019.2922081
TTAGGAC5900.019.0173113
AGGACGT7400.018.4322855
CGAATGC8000.018.42485243
ATACGAA8400.018.33318740
GGACGTG7400.018.134996
GCAATAC8550.018.01155337
CAATACG8600.017.90683438
CGCCGGT8450.017.7040027
CCGGTCC8850.017.6495769
GATATAC3000.017.604141
Produced by FastQC (version 0.11.5)