##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1851037
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21745756567805	32.0	32.0	32.0	32.0	32.0
2	31.328890778520364	32.0	32.0	32.0	32.0	32.0
3	31.4469516276552	32.0	32.0	32.0	32.0	32.0
4	31.57064499521079	32.0	32.0	32.0	32.0	32.0
5	31.480189753095157	32.0	32.0	32.0	32.0	32.0
6	35.09078424688431	36.0	36.0	36.0	36.0	36.0
7	35.11025495438503	36.0	36.0	36.0	36.0	36.0
8	35.0392666381061	36.0	36.0	36.0	36.0	36.0
9	35.18958454098973	36.0	36.0	36.0	36.0	36.0
10	35.04956572991247	36.0	36.0	36.0	36.0	36.0
11	35.1943370121721	36.0	36.0	36.0	36.0	36.0
12	35.09491598493169	36.0	36.0	36.0	36.0	36.0
13	35.14534555495109	36.0	36.0	36.0	36.0	36.0
14	35.10219082600726	36.0	36.0	36.0	36.0	36.0
15	35.05707125249252	36.0	36.0	36.0	36.0	36.0
16	35.07368950485593	36.0	36.0	36.0	36.0	36.0
17	35.04478354565576	36.0	36.0	36.0	36.0	36.0
18	35.06302899401795	36.0	36.0	36.0	36.0	36.0
19	35.04567547812388	36.0	36.0	36.0	36.0	36.0
20	35.03825207167658	36.0	36.0	36.0	36.0	36.0
21	35.021357757840605	36.0	36.0	36.0	36.0	36.0
22	35.00552285016453	36.0	36.0	36.0	36.0	36.0
23	34.95590471719366	36.0	36.0	36.0	32.0	36.0
24	34.93188520812928	36.0	36.0	36.0	32.0	36.0
25	34.9064902538415	36.0	36.0	36.0	32.0	36.0
26	34.855736541192854	36.0	36.0	36.0	32.0	36.0
27	34.83551220207916	36.0	36.0	36.0	32.0	36.0
28	34.8145174839833	36.0	36.0	36.0	32.0	36.0
29	34.78166454803443	36.0	36.0	36.0	32.0	36.0
30	34.765047376146455	36.0	36.0	36.0	32.0	36.0
31	34.76794142958785	36.0	36.0	36.0	32.0	36.0
32	34.72249015011585	36.0	36.0	36.0	32.0	36.0
33	34.70486813607724	36.0	36.0	36.0	32.0	36.0
34	34.683959856015846	36.0	36.0	36.0	32.0	36.0
35	34.64842139838372	36.0	36.0	36.0	32.0	36.0
36	34.61496447666902	36.0	36.0	36.0	32.0	36.0
37	34.59316264342636	36.0	36.0	36.0	32.0	36.0
38	34.54193784348989	36.0	36.0	36.0	32.0	36.0
39	34.49844114407222	36.0	36.0	36.0	32.0	36.0
40	34.479433420293596	36.0	36.0	36.0	32.0	36.0
41	34.45556841921582	36.0	36.0	36.0	32.0	36.0
42	34.38608142354799	36.0	36.0	36.0	32.0	36.0
43	34.39645290720823	36.0	36.0	36.0	32.0	36.0
44	34.35728999474349	36.0	36.0	36.0	32.0	36.0
45	34.285923512063775	36.0	36.0	36.0	32.0	36.0
46	34.30164605029505	36.0	36.0	36.0	32.0	36.0
47	34.26123951060946	36.0	36.0	36.0	32.0	36.0
48	34.235479895863776	36.0	36.0	36.0	32.0	36.0
49	34.22370757580751	36.0	36.0	36.0	32.0	36.0
50	33.68330508790478	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	8.0
21	48.0
22	173.0
23	480.0
24	1311.0
25	3153.0
26	6522.0
27	12281.0
28	20550.0
29	31112.0
30	44450.0
31	64237.0
32	95798.0
33	154360.0
34	338387.0
35	1078164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.378578969948265	17.914166318157797	11.56087666460263	26.146378047291304
2	16.13227903700014	20.458722634559468	36.74972960065177	26.659268727788625
3	18.88885527393427	24.148959389053	28.393195564842888	28.568989772169846
4	12.334416692958307	15.416643256302432	35.88689984830084	36.36204020243842
5	14.325483499249339	36.73324736350489	33.37858724595997	15.562681891285804
6	34.84494055208122	35.126729305598175	16.466544068389958	13.561786073930651
7	30.2655377853267	30.281312735138595	20.884466860720156	18.568682618814545
8	28.30705166887534	32.89269744472963	19.564006554163964	19.236244332231067
9	27.32268452764586	14.083619074064972	18.665753304769165	39.927943093520014
10	15.945602383960992	26.52178211456605	31.48602648137233	26.046589020100626
11	37.74576088970669	20.9927732400811	21.95915046538778	19.30231540482443
12	24.57730450552852	23.680023684021442	28.754314473454613	22.988357336995424
13	29.677202562671628	19.18746086653049	25.553946247427795	25.58139032337009
14	23.572408331113856	19.678915116229444	24.817548217566692	31.93112833509001
15	25.370589566821195	27.40863634816592	22.380751978485574	24.840022106527314
16	25.979599543391085	25.661561600335382	24.172396337836574	24.186442518436962
17	24.02701836862256	26.052369563655397	25.56777633294202	24.35283573478002
18	25.23250480676507	24.403023818540635	26.910213031938312	23.454258342755978
19	25.381070178499943	25.404246376490587	25.288149291451223	23.926534153558247
20	25.41688793903093	24.154838612086092	25.686196440157598	24.74207700872538
21	26.469811246344616	24.47671224292113	24.34008612469659	24.713390386037666
22	25.438497447646913	24.795668590093012	25.054496479540926	24.711337482719145
23	23.773160666156322	24.404860626772994	25.694678172289372	26.127300534781316
24	24.301513151817062	25.39819571407811	25.450166582299545	24.850124551805287
25	24.828190900560067	24.566013537276675	25.373074660312028	25.23272090185123
26	24.064402818528208	25.511105396596612	25.94037828525308	24.484113499622104
27	25.271834112446157	25.00457851463801	25.186638624727653	24.536948748188177
28	24.214967069810058	24.711067363861446	25.896619030305718	25.17734653602278
29	24.083851376282592	25.077132439816168	25.93713685896068	24.90187932494056
30	24.323230707976123	25.04941824501617	26.246476974798448	24.380874072209256
31	24.778975244687167	24.98394143390975	24.944828223314822	25.292255098088262
32	24.33851943532193	25.16340840296547	25.11683991189803	25.381232249814563
33	23.976614189775784	24.724627330517976	25.751835322578643	25.546923157127598
34	24.601074965006102	25.103009826383804	26.079813639597692	24.216101569012398
35	25.54616682432604	24.7847017644704	25.643517660641034	24.02561375056252
36	24.126476132027616	25.596246860543577	25.463078263697593	24.814198743731218
37	25.491224648669906	25.150928911739744	25.135856279480095	24.221990160110252
38	24.470445485422495	24.899394231449723	25.43250080900598	25.197659474121803
39	25.141744870577952	24.593133470589727	25.18863750427463	25.076484154557686
40	25.46664383261923	24.90295980037136	25.40057276002587	24.229823606983544
41	24.105136742269334	25.230289832131938	26.226380131785586	24.438193293813143
42	25.818284561572785	25.549408250618438	25.344496085167396	23.287811102641385
43	24.686972761754628	24.193681703823316	25.647677491049613	25.471668043372446
44	24.19022418244476	24.998095662053217	25.632334739932265	25.17934541556976
45	24.393083444577282	25.359244574797803	25.37356087425589	24.874111106369025
46	24.018385419592963	24.755487749536474	25.966379835680854	25.25974699518971
47	24.595955994372883	24.530641219295575	26.26075343753444	24.612649348797106
48	25.030726020063348	25.80802004498019	24.818574669225953	24.342679265730506
49	24.057595823314173	25.31797041334128	25.71834058422387	24.906093179120678
50	23.88505680062408	26.175828022793723	24.764240133633272	25.174875042948923
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	24.0
2	29.0
3	131.0
4	233.0
5	279.5
6	326.0
7	314.0
8	302.0
9	403.0
10	504.0
11	740.5
12	977.0
13	1757.0
14	2537.0
15	3405.0
16	4273.0
17	4924.0
18	5575.0
19	6138.5
20	6702.0
21	7624.0
22	8546.0
23	9372.0
24	10198.0
25	11400.0
26	12602.0
27	15428.5
28	18255.0
29	21626.0
30	24997.0
31	28489.0
32	31981.0
33	37009.0
34	42037.0
35	48789.5
36	55542.0
37	64378.0
38	73214.0
39	77935.0
40	82656.0
41	88858.5
42	95061.0
43	97706.0
44	100351.0
45	112437.0
46	124523.0
47	134487.5
48	144452.0
49	145917.0
50	147382.0
51	136921.5
52	126461.0
53	122618.5
54	118776.0
55	120924.0
56	123072.0
57	120512.5
58	117953.0
59	107919.5
60	97886.0
61	86633.5
62	75381.0
63	65875.5
64	56370.0
65	48507.0
66	40644.0
67	35857.5
68	31071.0
69	29046.0
70	27021.0
71	21072.0
72	15123.0
73	13404.0
74	11685.0
75	8379.5
76	5074.0
77	4561.5
78	4049.0
79	3492.0
80	2935.0
81	2301.5
82	1668.0
83	1444.0
84	1220.0
85	1023.0
86	826.0
87	596.0
88	366.0
89	235.0
90	104.0
91	69.5
92	35.0
93	28.0
94	21.0
95	16.0
96	11.0
97	8.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01593701260428614
2	0.0038356877793366637
3	4.8621394385957707E-4
4	2.70118857699765E-4
5	0.0
6	2.70118857699765E-4
7	5.4023771539953014E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.70118857699765E-4
47	5.4023771539953014E-5
48	0.0
49	0.0
50	5.4023771539953014E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1851037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.81509421167435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77696957764468	39.01428942374869
2	13.641024758384967	13.863399164822287
3	4.2853029040964215	6.53274212391664
4	1.7837177446182728	3.6255914095925124
5	0.9126975142767908	2.3189405087368056
6	0.5686432359958746	1.7337395765977048
7	0.3598565027406778	1.2800299462615836
8	0.26416913819429294	1.0739023716127853
9	0.1906620314153858	0.8719658180065839
>10	1.0283950520274854	9.516556778671406
>50	0.08836390051925114	3.124248583767634
>100	0.0828749794004684	9.066082218908146
>500	0.012403880490864064	4.272431136808429
>1k	0.00491878019465299	3.7060809385488267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2507	0.13543759525066218	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2482	0.13408700096216336	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2348	0.12684781557580968	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2332	0.12598343523117042	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2230	0.1204730105340952	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2088	0.11280163497542188	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2041	0.11026251771304409	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1989	0.10745328159296655	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1982	0.10707511519218688	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1885	0.10183480935281142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.402377153995301E-5	0.0
12	0.0	0.0	0.0	5.402377153995301E-5	0.0
13	0.0	0.0	0.0	5.402377153995301E-5	0.0
14	0.0	0.0	0.0	5.402377153995301E-5	0.0
15	0.0	0.0	0.0	1.0804754307990601E-4	5.402377153995301E-5
16	0.0	0.0	0.0	1.6207131461985904E-4	5.402377153995301E-5
17	0.0	0.0	0.0	2.7011885769976507E-4	5.402377153995301E-5
18	0.0	0.0	0.0	3.241426292397181E-4	5.402377153995301E-5
19	0.0	0.0	0.0	4.8621394385957707E-4	5.402377153995301E-5
20	0.0	0.0	0.0	0.0011344992023390131	5.402377153995301E-5
21	0.0	0.0	0.0	0.0016207131461985903	5.402377153995301E-5
22	5.402377153995301E-5	0.0	0.0	0.0023770459477579324	5.402377153995301E-5
23	5.402377153995301E-5	0.0	0.0	0.0034034976070170396	5.402377153995301E-5
24	5.402377153995301E-5	0.0	0.0	0.00556444846861516	5.402377153995301E-5
25	5.402377153995301E-5	0.0	0.0	0.007563328015593422	5.402377153995301E-5
26	5.402377153995301E-5	0.0	0.0	0.00950818379103173	1.0804754307990601E-4
27	5.402377153995301E-5	0.0	0.0	0.011885229738789662	1.0804754307990601E-4
28	5.402377153995301E-5	0.0	0.0	0.018206011008964164	1.0804754307990601E-4
29	5.402377153995301E-5	0.0	0.0	0.030199288290833733	1.0804754307990601E-4
30	5.402377153995301E-5	0.0	0.0	0.05418584285457287	1.0804754307990601E-4
31	5.402377153995301E-5	0.0	0.0	0.10269918969745068	1.0804754307990601E-4
32	5.402377153995301E-5	0.0	0.0	0.15499420054812518	1.0804754307990601E-4
33	5.402377153995301E-5	0.0	0.0	0.21339389758281438	1.0804754307990601E-4
34	5.402377153995301E-5	0.0	0.0	0.2728200462767627	1.0804754307990601E-4
35	5.402377153995301E-5	0.0	0.0	0.34477970996798013	1.0804754307990601E-4
36	5.402377153995301E-5	0.0	0.0	0.4520709202463268	1.0804754307990601E-4
37	5.402377153995301E-5	0.0	0.0	0.5945316057971829	1.0804754307990601E-4
38	5.402377153995301E-5	0.0	0.0	0.7860458759063162	1.0804754307990601E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	60	2.86901E-4	22.005175	1
TAGGACG	630	0.0	21.301992	4
CGCAATA	750	0.0	21.119831	36
TCTAGCG	765	0.0	19.84298	28
CTAGCGG	765	0.0	19.84298	29
TAGCGGC	775	0.0	19.58694	30
CGGTCCA	775	0.0	19.58694	10
GTATAAT	205	0.0	19.321617	1
GCCGGTC	820	0.0	19.31692	8
GTCCTAC	730	0.0	19.292208	1
TTAGGAC	590	0.0	19.017311	3
AGGACGT	740	0.0	18.432285	5
CGAATGC	800	0.0	18.424852	43
ATACGAA	840	0.0	18.333187	40
GGACGTG	740	0.0	18.13499	6
GCAATAC	855	0.0	18.011553	37
CAATACG	860	0.0	17.906834	38
CGCCGGT	845	0.0	17.704002	7
CCGGTCC	885	0.0	17.649576	9
GATATAC	300	0.0	17.60414	1
>>END_MODULE