FastQCFastQC Report
Thu 2 Feb 2017
SRR4062651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309879
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19430.14833431179521162No Hit
GCGCAAGACGGACCAGAGCGAAAGC14110.10771987336234874No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13630.10405541275186486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1058.913048E-1114.4676041
CTCGACG759.654868E-713.93518413
GGTATAT551.9696668E-413.8099871
ACGCTTA707.2333314E-613.576344517
TCAGTAG802.0077587E-613.0572473
CGGTCGG1301.9826984E-1012.42282710
CGTCGTA2000.012.34975210
AATCGTT701.089295E-412.21590713
CGTTATT1251.4206307E-912.1541812
TTAGGGC550.003077256712.0860463
CTTATAC550.003077256712.0860463
GTCGTAG2050.012.04899811
CGCTTAC951.0338517E-612.00526418
CCGTCGT2100.011.7616689
CGACGCT907.440569E-611.61354115
TAGAACT1604.5474735E-1111.2792994
CAAGACG2700.011.2573134
TCAGTAC1354.7530193E-911.2547313
GTCCTAA1105.0035123E-711.2206141
GACGCTT855.303605E-511.18009116
Produced by FastQC (version 0.11.5)