##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062651_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1309879
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21709562486306	32.0	32.0	32.0	32.0	32.0
2	30.967168723217945	32.0	32.0	32.0	32.0	32.0
3	30.942694706915677	32.0	32.0	32.0	32.0	32.0
4	30.952070382073458	32.0	32.0	32.0	32.0	32.0
5	30.867188496036658	32.0	32.0	32.0	32.0	32.0
6	34.399094114799915	36.0	36.0	36.0	32.0	36.0
7	34.296009020680536	36.0	36.0	36.0	32.0	36.0
8	34.28400714875191	36.0	36.0	36.0	32.0	36.0
9	34.454504576376905	36.0	36.0	36.0	32.0	36.0
10	34.16544505255829	36.0	36.0	36.0	32.0	36.0
11	34.381213073879344	36.0	36.0	36.0	32.0	36.0
12	34.237923502857896	36.0	36.0	36.0	32.0	36.0
13	34.323611570229005	36.0	36.0	36.0	32.0	36.0
14	34.255365571934504	36.0	36.0	36.0	32.0	36.0
15	34.24104058466469	36.0	36.0	36.0	32.0	36.0
16	34.23939157738997	36.0	36.0	36.0	32.0	36.0
17	34.136424051381844	36.0	36.0	36.0	32.0	36.0
18	34.17594831278309	36.0	36.0	36.0	32.0	36.0
19	34.171534164606044	36.0	36.0	36.0	32.0	36.0
20	34.11582214845799	36.0	36.0	36.0	32.0	36.0
21	34.08026237537971	36.0	36.0	36.0	32.0	36.0
22	34.08477500593566	36.0	36.0	36.0	32.0	36.0
23	34.012296555636055	36.0	36.0	36.0	32.0	36.0
24	33.99407197153325	36.0	36.0	36.0	32.0	36.0
25	33.4926684067765	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	48.0
5	139.0
6	212.0
7	55.0
8	204.0
9	245.0
10	156.0
11	63.0
12	96.0
13	69.0
14	192.0
15	315.0
16	553.0
17	754.0
18	964.0
19	1369.0
20	1832.0
21	2279.0
22	3265.0
23	4598.0
24	6340.0
25	8694.0
26	12576.0
27	16625.0
28	23428.0
29	31974.0
30	43652.0
31	61791.0
32	94722.0
33	143471.0
34	330752.0
35	518442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.445813819609974	17.270924030472713	10.73675980827051	26.546502341646804
2	17.572325737992024	19.84726355472967	35.89733997639403	26.68307073088428
3	19.28861655166306	23.4935647351456	28.08055189515458	29.13726681803676
4	13.028784959955775	14.956817597010561	35.35734383363226	36.6570536094014
5	14.992111023031537	36.049188115061455	33.38740299154894	15.571297870358066
6	34.67722532703663	34.86022988611098	16.49431700575212	13.968227781100273
7	30.444899926737463	30.08783100876935	20.758660678339268	18.708608386153916
8	28.552478555152955	32.121652991580994	19.514772607445828	19.811095845820223
9	27.593453426695696	14.02651550176411	18.267329872618912	40.112701198921286
10	15.938738961845994	26.32578233024001	31.4863605754936	26.249118132420396
11	38.044362235538706	20.938990461049283	21.711049284578696	19.305598018833315
12	24.592722944580046	23.519824728106112	28.33338047409335	23.55407185322049
13	29.54018050960945	19.126405645205672	25.04257446327131	26.29083938191357
14	23.559172916066316	19.223716404779175	24.688307131844688	32.52880354730982
15	25.49934689179346	26.58926181575546	21.926856466764853	25.98453482568622
16	26.239613204605348	25.40301377568802	23.155827917015696	25.201545102690936
17	24.58495250266762	25.414588881313232	24.758156485900614	25.24230213011854
18	25.39229715515765	24.447405273843597	25.537994422837013	24.622303148161738
19	25.89341477118537	24.82878557969048	24.895444347191273	24.382355301932876
20	25.739935335437863	24.094428605278644	24.7252520465646	25.44038401271889
21	26.866460019034005	24.001421817250616	23.963583132355133	25.168535031360246
22	26.08606895792429	24.111418977191406	24.47955850780777	25.322953557076538
23	24.6877422491732	23.85413752031644	25.20606947419602	26.252050756314343
24	24.83835654975132	24.879254750689153	24.70136668791844	25.581022011641082
25	24.93246269968796	24.256172381792524	24.884456559794398	25.926908358725115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	79.0
2	287.5
3	496.0
4	496.0
5	496.0
6	1226.5
7	1957.0
8	1957.0
9	1957.0
10	2284.0
11	2611.0
12	2611.0
13	2611.0
14	3203.0
15	3795.0
16	3795.0
17	3795.0
18	5972.0
19	8149.0
20	8149.0
21	8149.0
22	13106.5
23	18064.0
24	18064.0
25	18064.0
26	26731.5
27	35399.0
28	35399.0
29	35399.0
30	43087.0
31	50775.0
32	50775.0
33	50775.0
34	64665.5
35	78556.0
36	78556.0
37	78556.0
38	90438.5
39	102321.0
40	102321.0
41	102321.0
42	116866.0
43	131411.0
44	131411.0
45	131411.0
46	149566.5
47	167722.0
48	167722.0
49	167722.0
50	169979.0
51	172236.0
52	172236.0
53	172236.0
54	161787.5
55	151339.0
56	151339.0
57	151339.0
58	140461.0
59	129583.0
60	129583.0
61	129583.0
62	116053.5
63	102524.0
64	102524.0
65	102524.0
66	86220.5
67	69917.0
68	69917.0
69	69917.0
70	53766.5
71	37616.0
72	37616.0
73	37616.0
74	29834.5
75	22053.0
76	22053.0
77	22053.0
78	17720.5
79	13388.0
80	13388.0
81	13388.0
82	9637.5
83	5887.0
84	5887.0
85	5887.0
86	4173.5
87	2460.0
88	2460.0
89	2460.0
90	1653.0
91	846.0
92	846.0
93	846.0
94	501.0
95	156.0
96	156.0
97	156.0
98	347.5
99	539.0
100	539.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00893212273805443
2	0.003130060104788305
3	0.004962290410030239
4	0.015421271735786281
5	0.03488871872898183
6	0.04748530207752014
7	0.06779252129395158
8	0.08955025616869955
9	0.09817700718921367
10	0.12031645671088703
11	0.11901862691134067
12	0.13283669713004026
13	0.1208508572165826
14	0.12367554560383058
15	0.11604125266532252
16	0.11352193599561487
17	0.12176697236920357
18	0.128790521872631
19	0.13161521025987896
20	0.12130891479289309
21	0.12932492237832655
22	0.14894505523029225
23	0.1393258461277721
24	0.1338291552120463
25	0.13077543803664307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1309879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.95006682440147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48026730897578	47.79737002249643
2	10.981531170702862	12.727609303529572
3	2.887266057176976	5.019517828596958
4	1.1690481955814072	2.7098568421955402
5	0.5965984614485146	1.728646035413826
6	0.38025640629396357	1.322153049308517
7	0.2612276416777319	1.0596711504123753
8	0.18311939604542432	0.8489424990141101
9	0.13186441755005404	0.6877396627907769
>10	0.7539076708210541	8.18776514662493
>50	0.08203199376641365	3.354968167293974
>100	0.08298627489690132	9.988767816726691
>500	0.008047937451166035	3.2105191019392785
>1k	0.0018470676117430243	1.356473373657046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1943	0.14833431179521162	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1411	0.10771987336234874	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1363	0.10405541275186486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
7	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
8	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
9	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
10	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
11	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
12	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
13	7.634292938508061E-5	7.634292938508061E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	105	8.913048E-11	14.467604	1
CTCGACG	75	9.654868E-7	13.935184	13
GGTATAT	55	1.9696668E-4	13.809987	1
ACGCTTA	70	7.2333314E-6	13.5763445	17
TCAGTAG	80	2.0077587E-6	13.057247	3
CGGTCGG	130	1.9826984E-10	12.422827	10
CGTCGTA	200	0.0	12.349752	10
AATCGTT	70	1.089295E-4	12.215907	13
CGTTATT	125	1.4206307E-9	12.154181	2
TTAGGGC	55	0.0030772567	12.086046	3
CTTATAC	55	0.0030772567	12.086046	3
GTCGTAG	205	0.0	12.048998	11
CGCTTAC	95	1.0338517E-6	12.005264	18
CCGTCGT	210	0.0	11.761668	9
CGACGCT	90	7.440569E-6	11.613541	15
TAGAACT	160	4.5474735E-11	11.279299	4
CAAGACG	270	0.0	11.257313	4
TCAGTAC	135	4.7530193E-9	11.254731	3
GTCCTAA	110	5.0035123E-7	11.220614	1
GACGCTT	85	5.303605E-5	11.180091	16
>>END_MODULE