FastQCFastQC Report
Thu 2 Feb 2017
SRR4062651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309879
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15290.11672833902978824No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13640.10413175568124995No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13620.10397906982247979No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG13490.10298661174047373No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13290.10145975315277213No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13240.10107803850584673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA3850.019.99982636
GTATCAA19850.019.621451
TAGCGGC4350.019.21822430
ATAAGGG1051.792414E-618.85773
GTTCGGT708.1242935E-418.8569811
TTATACC1303.5883204E-818.6152254
TCTAGCG4650.018.45145428
CAACGTA600.007412795418.3331749
GGCGTTA3150.018.15857342
CTAGCGG4650.017.9783429
ATACGAA4650.017.0321140
GTTATAT1702.4610927E-916.8272361
CATGGGT7950.016.603634
CAATACG4650.016.55899638
CGAATGC4950.016.44430243
TCTATAC1351.08528E-616.2967783
GCAATAC4900.016.16312437
TACGAAT4800.016.04152741
GTATTAG3300.016.0035271
GGGTATA1105.6112338E-516.0035251
Produced by FastQC (version 0.11.5)