##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062651_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1309879
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199787155912876	32.0	32.0	32.0	32.0	32.0
2	31.208696375772114	32.0	32.0	32.0	32.0	32.0
3	31.36379390768155	32.0	32.0	32.0	32.0	32.0
4	31.518092129120323	32.0	32.0	32.0	32.0	32.0
5	31.38261396663356	32.0	32.0	32.0	32.0	32.0
6	34.9916320515101	36.0	36.0	36.0	36.0	36.0
7	35.012124020615644	36.0	36.0	36.0	36.0	36.0
8	34.937414829919405	36.0	36.0	36.0	36.0	36.0
9	35.122867073981645	36.0	36.0	36.0	36.0	36.0
10	34.933994666682956	36.0	36.0	36.0	32.0	36.0
11	35.124112990589204	36.0	36.0	36.0	36.0	36.0
12	35.01409748534025	36.0	36.0	36.0	36.0	36.0
13	35.07788123941219	36.0	36.0	36.0	36.0	36.0
14	35.02730328526528	36.0	36.0	36.0	36.0	36.0
15	34.96765731796601	36.0	36.0	36.0	32.0	36.0
16	34.991543493712015	36.0	36.0	36.0	36.0	36.0
17	34.955369923481484	36.0	36.0	36.0	32.0	36.0
18	34.98058370276949	36.0	36.0	36.0	36.0	36.0
19	34.95962375150682	36.0	36.0	36.0	32.0	36.0
20	34.96057956498272	36.0	36.0	36.0	32.0	36.0
21	34.95363922927232	36.0	36.0	36.0	32.0	36.0
22	34.93078826364878	36.0	36.0	36.0	32.0	36.0
23	34.89042041287783	36.0	36.0	36.0	32.0	36.0
24	34.86824431875005	36.0	36.0	36.0	32.0	36.0
25	34.83943020691224	36.0	36.0	36.0	32.0	36.0
26	34.7893156543467	36.0	36.0	36.0	32.0	36.0
27	34.774890657839386	36.0	36.0	36.0	32.0	36.0
28	34.759326624825654	36.0	36.0	36.0	32.0	36.0
29	34.72758323478733	36.0	36.0	36.0	32.0	36.0
30	34.72067954368304	36.0	36.0	36.0	32.0	36.0
31	34.72315687174159	36.0	36.0	36.0	32.0	36.0
32	34.68533505766563	36.0	36.0	36.0	32.0	36.0
33	34.660502993024544	36.0	36.0	36.0	32.0	36.0
34	34.64984170293592	36.0	36.0	36.0	32.0	36.0
35	34.6330088504358	36.0	36.0	36.0	32.0	36.0
36	34.59749793683233	36.0	36.0	36.0	32.0	36.0
37	34.591130936521616	36.0	36.0	36.0	32.0	36.0
38	34.560073869418474	36.0	36.0	36.0	32.0	36.0
39	34.54849722760652	36.0	36.0	36.0	32.0	36.0
40	34.544672446844324	36.0	36.0	36.0	32.0	36.0
41	34.50595207648951	36.0	36.0	36.0	32.0	36.0
42	34.485079156166336	36.0	36.0	36.0	32.0	36.0
43	34.47889537888614	36.0	36.0	36.0	32.0	36.0
44	34.456188701399135	36.0	36.0	36.0	32.0	36.0
45	34.417559179130286	36.0	36.0	36.0	32.0	36.0
46	34.425669088518866	36.0	36.0	36.0	32.0	36.0
47	34.41057227423296	36.0	36.0	36.0	32.0	36.0
48	34.36148300720906	36.0	36.0	36.0	32.0	36.0
49	34.36114404460259	36.0	36.0	36.0	32.0	36.0
50	33.77640759184627	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	22.0
22	100.0
23	279.0
24	782.0
25	1909.0
26	3969.0
27	7347.0
28	12728.0
29	20790.0
30	31030.0
31	46702.0
32	71588.0
33	122667.0
34	267754.0
35	722203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87957694645252	17.589080380784843	11.06820104511207	26.46314162765058
2	17.036204587756924	20.224547538084977	35.890349464130566	26.848898410027534
3	18.98112415306804	23.642026910964788	28.38690714762859	28.98994178833858
4	13.059327753161368	15.394510625433247	35.66238330956518	35.8837783118402
5	15.03581628532101	36.0934864976078	33.11527247936642	15.755424737704779
6	34.99026241475319	35.03431248678307	16.283563368357086	13.691861730106659
7	30.69041544327029	30.02195624325319	20.59252846448295	18.69509984899357
8	28.716392888198072	32.184881198950436	19.305752668757954	19.792973244093538
9	27.768900791599833	13.793029737861284	18.06663058190871	40.37143888863017
10	15.961550647044497	26.18531940736511	31.46351685919081	26.38961308639958
11	38.27055781488214	20.67442870677368	21.677651141823024	19.37736233652116
12	24.711748184374283	23.333452937256034	28.36597884232055	23.588820036049132
13	29.808249464263493	18.986181166352008	24.906422654306237	26.299146715078265
14	23.728451253894445	19.0747389644387	24.60967768778643	32.58713209388043
15	25.787496402339453	26.447633712732245	21.76636162576849	25.998508259159813
16	26.49145455419928	25.365014631122417	22.91036042260392	25.233170392074385
17	24.902529164907598	25.18392920262101	24.59631767514404	25.317223957327357
18	25.704435295168487	24.336904401093538	25.364861945263645	24.593798358474334
19	26.154858578540463	24.752973366242227	24.65479635905301	24.437371696164302
20	26.07187381429888	23.905795878855987	24.49126980431017	25.53106050253497
21	27.163348675717376	23.944730772842377	23.853806343944747	25.0381142074955
22	26.210970631638496	24.073063237138697	24.3826338157952	25.333332315427608
23	24.955740186689	23.720664275097164	24.963527165486276	26.360068372727557
24	24.985437586219795	24.825117434511128	24.5340981876952	25.65534679157388
25	25.27447191687171	24.176431563526098	24.67143911765896	25.87765740194323
26	25.096974606051397	24.79122117386415	24.926042787158202	25.185761432926245
27	26.03813023951067	24.328201307143637	24.111921788195705	25.521746665149987
28	25.090638142912436	24.147268564500994	25.188357092525337	25.573736200061226
29	25.09911220807418	24.608074486269345	24.835881787554424	25.456931518102056
30	25.107509930306538	24.654261958547316	25.144383565199536	25.09384454594661
31	25.518234890398272	24.500659984624534	24.169026299375744	25.812078825601446
32	25.358372796265915	24.42263751079298	24.24666705856037	25.972322634380735
33	24.996278282192478	24.024509134049786	24.837255960283354	26.141956623474382
34	25.57533940157831	24.29354161720281	24.978032322069442	25.15308665914943
35	26.28960384890513	23.989467729462035	24.682585185349183	25.038343236283655
36	25.07216315400125	24.603264881718083	24.63525256913043	25.68931939515024
37	26.046069904166718	24.354310588993332	24.374617808209766	25.22500169863018
38	25.122473144466017	24.214603028218637	24.91459134775044	25.748332479564905
39	25.577247974812938	24.193837751425896	24.354921332428415	25.873992941332745
40	25.870023109004727	24.288044926287082	24.845500996656945	24.996430968051246
41	24.764272119791215	24.775341844552052	25.14957488439772	25.31081115125901
42	26.017670334435472	24.69380759596879	24.570284736223726	24.718237333372013
43	25.278670777987887	23.655543756331692	25.073995384306492	25.991790081373928
44	25.126824691440962	24.212312740337087	24.732055403590714	25.928807164631234
45	25.180570113728063	24.595783274638343	24.763050632921054	25.460595978712536
46	24.874625632788423	24.220823787271627	25.008225963201124	25.896324616738823
47	25.056054795900994	23.893581010154374	25.441739275154422	25.608624918790206
48	25.652064045610317	24.736177921777507	24.526311208897923	25.085446823714253
49	24.706098807599787	24.667774657048476	24.889550866912135	25.736575668439603
50	24.5497672302306	24.94468950543486	24.815898885239694	25.689644379094844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	6.5
2	7.0
3	30.5
4	54.0
5	77.0
6	100.0
7	107.0
8	114.0
9	159.0
10	204.0
11	291.5
12	379.0
13	685.5
14	992.0
15	1329.5
16	1667.0
17	2033.0
18	2399.0
19	2705.0
20	3011.0
21	3731.0
22	4451.0
23	4929.5
24	5408.0
25	6755.0
26	8102.0
27	10083.0
28	12064.0
29	13824.5
30	15585.0
31	17167.0
32	18749.0
33	21519.0
34	24289.0
35	28180.5
36	32072.0
37	38985.5
38	45899.0
39	50140.0
40	54381.0
41	60151.0
42	65921.0
43	67861.0
44	69801.0
45	76257.0
46	82713.0
47	88166.0
48	93619.0
49	96732.5
50	99846.0
51	96059.0
52	92272.0
53	91063.0
54	89854.0
55	91292.0
56	92730.0
57	91530.0
58	90330.0
59	82706.0
60	75082.0
61	66765.0
62	58448.0
63	51901.5
64	45355.0
65	38923.0
66	32491.0
67	29755.5
68	27020.0
69	24750.5
70	22481.0
71	18028.5
72	13576.0
73	12182.5
74	10789.0
75	8321.0
76	5853.0
77	5086.0
78	4319.0
79	3719.0
80	3119.0
81	2463.0
82	1807.0
83	1500.5
84	1194.0
85	964.5
86	735.0
87	558.5
88	382.0
89	259.5
90	137.0
91	89.0
92	41.0
93	29.5
94	18.0
95	12.5
96	7.0
97	6.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01244389748976814
2	0.0034354318223286276
3	3.0537171754032243E-4
4	2.2902878815524183E-4
5	0.0
6	4.5805757631048365E-4
7	7.634292938508061E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5268585877016122E-4
47	0.0
48	0.0
49	0.0
50	7.634292938508061E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1309879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.92886891768161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.24873162784534	50.72251056916245
2	10.674410014781419	13.007594571284098
3	2.7094586829299487	4.952527587903393
4	1.055367354708998	2.572093568602597
5	0.6014917388300994	1.8324105655123761
6	0.35165066627180674	1.285540641005419
7	0.2405012936706088	1.025743025761256
8	0.16355855736622363	0.7972350321705399
9	0.12129289440054028	0.6651214977219045
>10	0.6834483263456143	7.673106679716211
>50	0.07233457304715656	3.074251706615162
>100	0.07045696150965464	8.89250083332566
>500	0.006039151690431953	2.53561860734649
>1k	0.0012581566021733236	0.9637451138724816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1529	0.11672833902978824	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1364	0.10413175568124995	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1362	0.10397906982247979	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1349	0.10298661174047373	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1329	0.10145975315277213	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1324	0.10107803850584673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	7.634292938508061E-5
10	0.0	0.0	0.0	0.0	7.634292938508061E-5
11	0.0	0.0	0.0	0.0	7.634292938508061E-5
12	0.0	0.0	0.0	0.0	7.634292938508061E-5
13	0.0	0.0	0.0	0.0	7.634292938508061E-5
14	0.0	0.0	0.0	0.0	7.634292938508061E-5
15	0.0	0.0	0.0	0.0	7.634292938508061E-5
16	0.0	0.0	0.0	0.0	7.634292938508061E-5
17	0.0	0.0	0.0	0.0	7.634292938508061E-5
18	0.0	0.0	0.0	0.0	7.634292938508061E-5
19	0.0	0.0	0.0	7.634292938508061E-5	7.634292938508061E-5
20	0.0	0.0	0.0	3.81714646925403E-4	7.634292938508061E-5
21	0.0	0.0	0.0	5.344005056955643E-4	7.634292938508061E-5
22	0.0	0.0	0.0	7.63429293850806E-4	7.634292938508061E-5
23	0.0	0.0	0.0	0.001145143940776209	7.634292938508061E-5
24	0.0	0.0	0.0	0.0014505156583165316	7.634292938508061E-5
25	0.0	0.0	0.0	0.0016795444464717734	7.634292938508061E-5
26	0.0	0.0	0.0	0.0020612590933971765	7.634292938508061E-5
27	0.0	0.0	0.0	0.002977374246018144	7.634292938508061E-5
28	0.0	0.0	0.0	0.006718177785887094	7.634292938508061E-5
29	0.0	0.0	0.0	0.015497614665171364	7.634292938508061E-5
30	0.0	0.0	0.0	0.03298014549435482	7.634292938508061E-5
31	0.0	0.0	0.0	0.07428167029168344	7.634292938508061E-5
32	0.0	0.0	0.0	0.12016377085211688	7.634292938508061E-5
33	0.0	0.0	0.0	0.17192427697520152	7.634292938508061E-5
34	0.0	0.0	0.0	0.22971587451970754	7.634292938508061E-5
35	0.0	0.0	0.0	0.2924697624742438	7.634292938508061E-5
36	0.0	0.0	0.0	0.38331784844248973	7.634292938508061E-5
37	0.0	0.0	0.0	0.5185211763834675	7.634292938508061E-5
38	0.0	0.0	0.0	0.699911976602419	7.634292938508061E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	385	0.0	19.999826	36
GTATCAA	1985	0.0	19.62145	1
TAGCGGC	435	0.0	19.218224	30
ATAAGGG	105	1.792414E-6	18.8577	3
GTTCGGT	70	8.1242935E-4	18.85698	11
TTATACC	130	3.5883204E-8	18.615225	4
TCTAGCG	465	0.0	18.451454	28
CAACGTA	60	0.0074127954	18.333174	9
GGCGTTA	315	0.0	18.158573	42
CTAGCGG	465	0.0	17.97834	29
ATACGAA	465	0.0	17.03211	40
GTTATAT	170	2.4610927E-9	16.827236	1
CATGGGT	795	0.0	16.60363	4
CAATACG	465	0.0	16.558996	38
CGAATGC	495	0.0	16.444302	43
TCTATAC	135	1.08528E-6	16.296778	3
GCAATAC	490	0.0	16.163124	37
TACGAAT	480	0.0	16.041527	41
GTATTAG	330	0.0	16.003527	1
GGGTATA	110	5.6112338E-5	16.003525	1
>>END_MODULE