FastQCFastQC Report
Thu 2 Feb 2017
SRR4062650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338903
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25760.19239631250359435No Hit
TATCAACGCAGAGTACTTTTTTTTT16500.12323521569523707No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16360.12218958356206536No Hit
GTACATGGAAGCAGTGGTATCAACG13660.10202382099375384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGCGT400.00527583814.251121510
AACCGCG1301.8189894E-1213.885197
GACCGTC500.0014988913.30054957
CGCGGTC1301.4551915E-1113.154881510
TAGGACG1551.8189894E-1212.2549054
GCTGTCG550.003067111812.0914098
CGTATAA550.003077435812.0859842
TAGACAG1501.4551915E-1112.0311315
TAGAACC1750.011.9397784
CGCCGGT2400.011.8754917
CAAGACG2500.011.7769634
AATCGTA658.016837E-411.69322913
AGAACCG1900.011.4978945
CCATGCG752.071886E-411.4008989
GGACGTG2000.011.4004716
TCGGCAA600.00587148811.08420713
AGGACGT1803.6379788E-1211.0813055
CCGGTCC2750.011.0554169
CGAACGA951.3562536E-511.00251216
CCGATAA951.3585046E-511.0008669
Produced by FastQC (version 0.11.5)