##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062650_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1338903
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112586199298978	32.0	32.0	32.0	32.0	32.0
2	30.706401434607287	32.0	32.0	32.0	32.0	32.0
3	30.690402516089666	32.0	32.0	32.0	32.0	32.0
4	30.779888460926596	32.0	32.0	32.0	32.0	32.0
5	30.513111853509926	32.0	32.0	32.0	32.0	32.0
6	34.16664089930339	36.0	36.0	36.0	32.0	36.0
7	34.0457411776656	36.0	36.0	36.0	32.0	36.0
8	34.039482322468466	36.0	36.0	36.0	32.0	36.0
9	34.2421631738819	36.0	36.0	36.0	32.0	36.0
10	33.84143660892536	36.0	36.0	36.0	32.0	36.0
11	34.21988896880506	36.0	36.0	36.0	32.0	36.0
12	33.95351044847909	36.0	36.0	36.0	32.0	36.0
13	34.114272654553766	36.0	36.0	36.0	32.0	36.0
14	33.9928486230892	36.0	36.0	36.0	32.0	36.0
15	33.920333287773644	36.0	36.0	36.0	32.0	36.0
16	33.92531871240859	36.0	36.0	36.0	32.0	36.0
17	33.842140916855065	36.0	36.0	36.0	32.0	36.0
18	33.8502221594843	36.0	36.0	36.0	32.0	36.0
19	33.84655945949781	36.0	36.0	36.0	32.0	36.0
20	33.83223131175298	36.0	36.0	36.0	32.0	36.0
21	33.82326576309113	36.0	36.0	36.0	32.0	36.0
22	33.77272588081437	36.0	36.0	36.0	32.0	36.0
23	33.728155811137924	36.0	36.0	36.0	32.0	36.0
24	33.706435791091664	36.0	36.0	36.0	27.0	36.0
25	33.22318793818521	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	40.0
5	120.0
6	230.0
7	65.0
8	206.0
9	251.0
10	164.0
11	46.0
12	107.0
13	73.0
14	255.0
15	305.0
16	447.0
17	680.0
18	828.0
19	1269.0
20	1917.0
21	2881.0
22	4411.0
23	6451.0
24	9060.0
25	12659.0
26	17388.0
27	22963.0
28	30916.0
29	40793.0
30	53422.0
31	72228.0
32	102887.0
33	147109.0
34	309424.0
35	499302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.237426537154946	17.491253178285774	11.03757772047817	26.233742564081115
2	16.946196563481482	20.015087518812717	36.69926915162583	26.33944676607997
3	19.40260492991726	23.49247038120889	28.211758484545648	28.893166204328203
4	12.868298469288666	15.090265058369015	35.51374293730448	36.52769353503784
5	14.792619566695405	36.25358434855632	33.53097370257416	15.422822382174115
6	34.52029942151095	34.844812204011916	16.77558480296851	13.85930357150863
7	30.217109732755247	30.29461422270919	20.82994827304002	18.658327771495536
8	28.21089772820716	32.58733208243939	19.75757077393456	19.444199415418886
9	27.261540485804233	14.30454307922223	18.612502682008376	39.821413752965164
10	15.822139270000509	26.727863870618464	31.799066189292585	25.650930670088446
11	37.37205525179167	21.213852430576328	22.045231571768703	19.368860745863294
12	24.431132350631575	23.728358499173602	28.723534734842538	23.116974415352285
13	29.446835685357232	19.47030108697603	25.27129533802741	25.81156788963933
14	23.345083745993666	19.62411142801165	25.100990235242566	31.92981459075212
15	25.137674359482425	27.058498050314228	22.40960972637104	25.394217863832303
16	25.747080542482987	25.699824884889104	23.718975389672245	24.83411918295566
17	24.006114035435385	25.77788313487894	25.318881795564185	24.89712103412149
18	24.836182900800353	24.897955520722245	26.12286319832601	24.142998380151397
19	25.323875512745246	25.23480647489278	25.354313042892983	24.08700496946899
20	25.467949978650516	24.52333823636082	25.171973626846956	24.836738158141706
21	26.318402974604876	24.36160713618001	24.573544742466645	24.74644514674847
22	25.47826633316179	24.59092296276793	24.95429766506696	24.976513039003315
23	24.389780563325505	24.362781027029452	25.482850433039655	25.764587976605384
24	24.506322600717372	25.091166906487732	25.41597238218603	24.986538110608866
25	24.754687688130108	24.685137973919606	25.347729874892778	25.21244446305751
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	247.0
1	247.0
2	513.0
3	779.0
4	779.0
5	779.0
6	1897.5
7	3016.0
8	3016.0
9	3016.0
10	3247.5
11	3479.0
12	3479.0
13	3479.0
14	3885.0
15	4291.0
16	4291.0
17	4291.0
18	6662.5
19	9034.0
20	9034.0
21	9034.0
22	14347.0
23	19660.0
24	19660.0
25	19660.0
26	28622.0
27	37584.0
28	37584.0
29	37584.0
30	47191.5
31	56799.0
32	56799.0
33	56799.0
34	69507.5
35	82216.0
36	82216.0
37	82216.0
38	94263.5
39	106311.0
40	106311.0
41	106311.0
42	121654.0
43	136997.0
44	136997.0
45	136997.0
46	156764.5
47	176532.0
48	176532.0
49	176532.0
50	178568.0
51	180604.0
52	180604.0
53	180604.0
54	168424.0
55	156244.0
56	156244.0
57	156244.0
58	143807.5
59	131371.0
60	131371.0
61	131371.0
62	115089.5
63	98808.0
64	98808.0
65	98808.0
66	81710.0
67	64612.0
68	64612.0
69	64612.0
70	48981.5
71	33351.0
72	33351.0
73	33351.0
74	25692.0
75	18033.0
76	18033.0
77	18033.0
78	14427.0
79	10821.0
80	10821.0
81	10821.0
82	7680.0
83	4539.0
84	4539.0
85	4539.0
86	3309.0
87	2079.0
88	2079.0
89	2079.0
90	1422.5
91	766.0
92	766.0
93	766.0
94	477.0
95	188.0
96	188.0
97	188.0
98	365.0
99	542.0
100	542.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0097841292461067
2	0.0035850244565887143
3	0.004257216542199099
4	0.013369153702695414
5	0.03323616423295788
6	0.04399123760272402
7	0.06841421671323464
8	0.08910279534813201
9	0.09604878023277265
10	0.11852987109596438
11	0.12002363128620969
12	0.13242184086524567
13	0.12062113536230781
14	0.12286177564767575
15	0.11262951834449546
16	0.11180795023986055
17	0.12338459171426161
18	0.13003182456085316
19	0.12980776053231638
20	0.12204020754304082
21	0.1282393123325588
22	0.1487038269389194
23	0.1376500015311042
24	0.13406497707451548
25	0.12921025645621825
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1338903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.06239704532824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64450306137968	48.22120056379724
2	11.499037556079534	13.583214435526209
3	2.9785430235932804	5.277596720281765
4	1.2352197311698974	2.918201528023203
5	0.6815800482203249	2.01278757130814
6	0.41026589423810367	1.4538772283788515
7	0.264913935625979	1.0952516434147468
8	0.2124899880233412	1.0040134432633296
9	0.14240320418790453	0.7569607127645671
>10	0.7786433413409469	8.393444775120203
>50	0.07113301166203298	2.931199794569941
>100	0.07442100531741931	8.944754439993897
>500	0.00494447717227352	1.9474835342516243
>1k	0.0019017219893359694	1.460013609306297
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2576	0.19239631250359435	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1650	0.12323521569523707	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1636	0.12218958356206536	No Hit
GTACATGGAAGCAGTGGTATCAACG	1366	0.10202382099375384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4937601902452978E-4
2	0.0	0.0	0.0	0.0	1.4937601902452978E-4
3	0.0	0.0	0.0	0.0	1.4937601902452978E-4
4	0.0	0.0	0.0	0.0	1.4937601902452978E-4
5	0.0	0.0	0.0	0.0	1.4937601902452978E-4
6	7.468800951226489E-5	0.0	0.0	0.0	2.2406402853679467E-4
7	7.468800951226489E-5	0.0	0.0	0.0	2.2406402853679467E-4
8	7.468800951226489E-5	0.0	0.0	0.0	2.2406402853679467E-4
9	7.468800951226489E-5	0.0	0.0	0.0	2.2406402853679467E-4
10	7.468800951226489E-5	0.0	0.0	7.468800951226489E-5	2.2406402853679467E-4
11	7.468800951226489E-5	0.0	0.0	7.468800951226489E-5	2.2406402853679467E-4
12	7.468800951226489E-5	0.0	0.0	7.468800951226489E-5	4.4812805707358935E-4
13	7.468800951226489E-5	0.0	0.0	7.468800951226489E-5	5.975040760981191E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGCGT	40	0.005275838	14.2511215	10
AACCGCG	130	1.8189894E-12	13.88519	7
GACCGTC	50	0.00149889	13.3005495	7
CGCGGTC	130	1.4551915E-11	13.1548815	10
TAGGACG	155	1.8189894E-12	12.254905	4
GCTGTCG	55	0.0030671118	12.091409	8
CGTATAA	55	0.0030774358	12.085984	2
TAGACAG	150	1.4551915E-11	12.031131	5
TAGAACC	175	0.0	11.939778	4
CGCCGGT	240	0.0	11.875491	7
CAAGACG	250	0.0	11.776963	4
AATCGTA	65	8.016837E-4	11.693229	13
AGAACCG	190	0.0	11.497894	5
CCATGCG	75	2.071886E-4	11.400898	9
GGACGTG	200	0.0	11.400471	6
TCGGCAA	60	0.005871488	11.084207	13
AGGACGT	180	3.6379788E-12	11.081305	5
CCGGTCC	275	0.0	11.055416	9
CGAACGA	95	1.3562536E-5	11.002512	16
CCGATAA	95	1.3585046E-5	11.000866	9
>>END_MODULE