FastQCFastQC Report
Thu 2 Feb 2017
SRR4062650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338903
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGA2000.018.7012562
GTCCTAC3100.018.4535391
CGCAATA3650.018.08205236
GCATTCG3450.017.85493722
ATACGAA3900.016.92294740
GGACCGT1053.773872E-516.7624026
GTATTAC1206.401213E-616.5017241
GTAATAG800.001987113416.5017241
ATAATAC1206.4057604E-616.500493
AAGGCGT1753.6252459E-916.3433446
GCATATA1501.8358332E-716.1350171
TCTAGCG4100.016.09743928
CGAATGC4100.016.09743943
TACGAAT4250.016.04693641
GTATCAA27550.015.8129021
CCGCTAG4200.015.71416636
ATACCGT2950.015.6614826
CGCCGGT4500.015.6443247
ATATAGT1704.489266E-815.5298746
CAATACG4250.015.52929338
Produced by FastQC (version 0.11.5)