##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1338903
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226221018251508	32.0	32.0	32.0	32.0	32.0
2	31.305185663188446	32.0	32.0	32.0	32.0	32.0
3	31.419916902120615	32.0	32.0	32.0	32.0	32.0
4	31.55011079966211	32.0	32.0	32.0	32.0	32.0
5	31.45202975869051	32.0	32.0	32.0	32.0	32.0
6	35.043937462235874	36.0	36.0	36.0	36.0	36.0
7	35.06232340953751	36.0	36.0	36.0	36.0	36.0
8	34.98883937073858	36.0	36.0	36.0	36.0	36.0
9	35.1502080434505	36.0	36.0	36.0	36.0	36.0
10	34.99722534044662	36.0	36.0	36.0	36.0	36.0
11	35.15232992980074	36.0	36.0	36.0	36.0	36.0
12	35.05702504214271	36.0	36.0	36.0	36.0	36.0
13	35.10150847372812	36.0	36.0	36.0	36.0	36.0
14	35.06879885996222	36.0	36.0	36.0	36.0	36.0
15	35.01560157830702	36.0	36.0	36.0	36.0	36.0
16	35.03355284139329	36.0	36.0	36.0	36.0	36.0
17	35.00441480824227	36.0	36.0	36.0	36.0	36.0
18	35.02340946282143	36.0	36.0	36.0	36.0	36.0
19	35.011854480869786	36.0	36.0	36.0	36.0	36.0
20	34.99417582901823	36.0	36.0	36.0	36.0	36.0
21	34.99447756857666	36.0	36.0	36.0	36.0	36.0
22	34.97926735543949	36.0	36.0	36.0	36.0	36.0
23	34.934188660418265	36.0	36.0	36.0	32.0	36.0
24	34.91713888160681	36.0	36.0	36.0	32.0	36.0
25	34.88658102939496	36.0	36.0	36.0	32.0	36.0
26	34.83136791836302	36.0	36.0	36.0	32.0	36.0
27	34.822927426408036	36.0	36.0	36.0	32.0	36.0
28	34.80696659877527	36.0	36.0	36.0	32.0	36.0
29	34.78366842108801	36.0	36.0	36.0	32.0	36.0
30	34.769013139861514	36.0	36.0	36.0	32.0	36.0
31	34.765272017465044	36.0	36.0	36.0	32.0	36.0
32	34.72606006559101	36.0	36.0	36.0	32.0	36.0
33	34.710269526619925	36.0	36.0	36.0	32.0	36.0
34	34.694473012608086	36.0	36.0	36.0	32.0	36.0
35	34.669286722040354	36.0	36.0	36.0	32.0	36.0
36	34.63121077479101	36.0	36.0	36.0	32.0	36.0
37	34.62482943125828	36.0	36.0	36.0	32.0	36.0
38	34.593118396179555	36.0	36.0	36.0	32.0	36.0
39	34.58412222543381	36.0	36.0	36.0	32.0	36.0
40	34.57085165990367	36.0	36.0	36.0	32.0	36.0
41	34.54569225701937	36.0	36.0	36.0	32.0	36.0
42	34.51358164108976	36.0	36.0	36.0	32.0	36.0
43	34.500189334104114	36.0	36.0	36.0	32.0	36.0
44	34.47520768868245	36.0	36.0	36.0	32.0	36.0
45	34.43333833742997	36.0	36.0	36.0	32.0	36.0
46	34.42808627660107	36.0	36.0	36.0	32.0	36.0
47	34.402103811851944	36.0	36.0	36.0	32.0	36.0
48	34.34630290618514	36.0	36.0	36.0	32.0	36.0
49	34.34050338224651	36.0	36.0	36.0	32.0	36.0
50	33.80436222788357	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	11.0
21	34.0
22	109.0
23	309.0
24	918.0
25	2166.0
26	4390.0
27	8150.0
28	14083.0
29	21576.0
30	31889.0
31	46511.0
32	68880.0
33	113597.0
34	247708.0
35	778568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8446857223959	18.086210180734366	11.525765571951998	26.543338524917736
2	16.412057801801694	20.602367253712327	36.26549929753199	26.720075646953994
3	18.83483195495994	23.885780608963948	28.53161968396374	28.747767752112374
4	12.675769156942895	15.6168379887684	35.794132650584324	35.91326020370438
5	14.571705343852393	36.51317533831801	33.36858607382312	15.546533244006474
6	34.732921351503556	35.16589065303153	16.449547163554396	13.651640831910516
7	30.280983760586093	30.46964567261407	20.661840327491984	18.58753023930785
8	28.250291469957123	32.85413506430264	19.41186180029472	19.48371166544552
9	27.422823012570742	14.157933771154447	18.437257964169174	39.98198525210564
10	15.792555547339873	26.833534617518968	31.76354074940455	25.610369085736608
11	37.58113918633389	21.097644862996052	22.08091250822502	19.240303442445047
12	24.65652851625547	23.608879806826934	28.616785532633806	23.11780614428379
13	29.704392327151407	19.366899618568336	25.129826432534696	25.79888162174556
14	23.4759351498951	19.425006889968877	25.118324479069802	31.980733481066213
15	25.330214362056104	26.964612074212997	22.363083808162354	25.34208975556855
16	25.768035473816997	25.665936964813728	23.6970116580514	24.869015903317866
17	24.054169719539058	25.792682516956045	25.22460551660576	24.92854224689914
18	25.038632372920215	24.703880714286246	26.199284040740817	24.058202872052718
19	25.521938482474084	25.10301343711979	25.436271335563514	23.938776744842606
20	25.553382134478746	24.396688931162302	25.3228949371239	24.72703399723505
21	26.448069800426172	24.293694166044887	24.555326263366354	24.702909770162588
22	25.52529944290214	24.55495282331879	25.011894065514827	24.90785366826424
23	24.579674554467353	24.178599943386487	25.503042416067483	25.73868308607868
24	24.72927463752042	25.071868537153176	25.330214362056104	24.868642463270305
25	24.977164141091627	24.443667689145517	25.35896924571832	25.220198924044535
26	24.525376371551936	25.16672230923375	25.717770443415244	24.59013087579907
27	25.291003157062164	24.85930646208127	25.01645003409508	24.83324034676149
28	24.53411486866487	24.631209281030813	25.700666889236935	25.134008961067384
29	24.563691320431726	24.95303991401916	25.54852741386045	24.934741351688658
30	24.687897480250623	24.92689911068987	25.75190286376235	24.633300545297157
31	24.7870084688734	24.872227487726892	24.981421357633824	25.359342685765885
32	24.634868993496912	24.96155434710356	25.011445937457754	25.39213072194177
33	24.454348074505774	24.50834750538314	25.488478254212595	25.548826165898504
34	24.99023454275627	24.722627404673826	25.71732231535817	24.56981573721173
35	25.510585905028222	24.579226426410276	25.51805470597945	24.392132962582057
36	24.61485260694763	25.02840011561704	25.250148815858953	25.10659846157638
37	25.38354160084786	24.78738190892096	24.992400495032125	24.83667599519905
38	24.61888575946129	24.707092298695272	25.46360714704501	25.21041479479843
39	24.96446717947454	24.664520133273285	25.14610841860837	25.224904268643805
40	25.33185749826537	24.71448641163699	25.509092144837975	24.444563945259663
41	24.37151907195667	24.90344707570302	25.885669088798814	24.839364763541496
42	25.320878360867066	25.071196345067566	25.306463575031202	24.301461719034165
43	24.666536709530114	24.25059918455631	25.649879042768596	25.432985063144976
44	24.254408273041438	24.780585300055343	25.62329011138223	25.34171631552099
45	24.438215464451122	24.96835095596918	25.575340409275356	25.018093170304347
46	24.355515721861227	24.660019418925984	25.651206214056316	25.33325864515647
47	24.523602175513968	24.493428197134666	25.942451351928668	25.040518275422695
48	24.887239777638857	25.29585787768046	25.122133567554933	24.69476877712575
49	24.09659250894202	25.07552824961928	25.582286394159997	25.245592847278704
50	24.012006862344563	25.431379915318608	25.28738121787944	25.269232004457386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	35.5
2	41.0
3	143.0
4	245.0
5	279.0
6	313.0
7	270.0
8	227.0
9	316.0
10	405.0
11	589.5
12	774.0
13	1358.0
14	1942.0
15	2560.0
16	3178.0
17	3535.0
18	3892.0
19	4131.0
20	4370.0
21	5052.5
22	5735.0
23	6262.5
24	6790.0
25	7989.5
26	9189.0
27	11469.5
28	13750.0
29	15755.0
30	17760.0
31	19619.0
32	21478.0
33	24710.0
34	27942.0
35	32299.5
36	36657.0
37	43129.5
38	49602.0
39	53244.5
40	56887.0
41	62922.0
42	68957.0
43	71003.0
44	73049.0
45	80273.0
46	87497.0
47	93043.0
48	98589.0
49	100684.5
50	102780.0
51	99783.5
52	96787.0
53	95389.0
54	93991.0
55	93824.0
56	93657.0
57	91279.0
58	88901.0
59	81793.0
60	74685.0
61	66163.5
62	57642.0
63	50083.0
64	42524.0
65	36347.5
66	30171.0
67	25850.5
68	21530.0
69	19342.5
70	17155.0
71	13468.0
72	9781.0
73	8920.5
74	8060.0
75	5939.5
76	3819.0
77	3372.0
78	2925.0
79	2533.5
80	2142.0
81	1702.0
82	1262.0
83	1086.5
84	911.0
85	709.0
86	507.0
87	381.5
88	256.0
89	166.0
90	76.0
91	50.0
92	24.0
93	16.0
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014788225883428449
2	0.0038837764946377746
3	4.481280570735893E-4
4	1.4937601902452978E-4
5	0.0
6	6.721920856103841E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.2406402853679465E-4
47	7.468800951226489E-5
48	0.0
49	0.0
50	1.4937601902452978E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1338903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.82731584202895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12013383091232	50.77267451353503
2	11.304251715529581	13.9782308234769
3	2.950755869181649	5.473119452898433
4	1.2057890542110867	2.982028027742809
5	0.6334874685893522	1.9583414901220637
6	0.3766845802567651	1.3973637909814267
7	0.24949897668704096	1.0798096423724888
8	0.17532846177762051	0.8672070545937635
9	0.14776275665802407	0.8222197163026069
>10	0.7077861980484995	7.932847079214149
>50	0.06207890226979113	2.7136337549355036
>100	0.060976219964162595	7.590965627386219
>500	0.004858636368143788	2.015810346150718
>1k	6.073295460179736E-4	0.41574868028799544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.468800951226489E-5
2	0.0	0.0	0.0	0.0	1.4937601902452978E-4
3	0.0	0.0	0.0	0.0	1.4937601902452978E-4
4	0.0	0.0	0.0	0.0	1.4937601902452978E-4
5	0.0	0.0	0.0	0.0	1.4937601902452978E-4
6	0.0	0.0	0.0	0.0	2.2406402853679467E-4
7	0.0	0.0	0.0	7.468800951226489E-5	2.9875203804905956E-4
8	0.0	0.0	0.0	7.468800951226489E-5	2.9875203804905956E-4
9	0.0	0.0	0.0	7.468800951226489E-5	2.9875203804905956E-4
10	0.0	0.0	0.0	2.2406402853679467E-4	2.9875203804905956E-4
11	0.0	0.0	0.0	2.2406402853679467E-4	2.9875203804905956E-4
12	0.0	0.0	0.0	2.2406402853679467E-4	5.228160665858543E-4
13	0.0	0.0	0.0	2.2406402853679467E-4	6.721920856103841E-4
14	0.0	0.0	0.0	2.2406402853679467E-4	6.721920856103841E-4
15	0.0	0.0	0.0	2.9875203804905956E-4	6.721920856103841E-4
16	7.468800951226489E-5	0.0	0.0	2.9875203804905956E-4	6.721920856103841E-4
17	7.468800951226489E-5	0.0	0.0	2.9875203804905956E-4	8.962561141471787E-4
18	7.468800951226489E-5	0.0	0.0	5.975040760981191E-4	9.709441236594436E-4
19	7.468800951226489E-5	0.0	0.0	8.215681046349139E-4	0.0010456321331717086
20	7.468800951226489E-5	0.0	0.0	9.709441236594436E-4	0.0011950081521962383
21	7.468800951226489E-5	0.0	0.0	0.0011203201426839733	0.0011950081521962383
22	7.468800951226489E-5	0.0	0.0	0.0019418882473188873	0.0013443841712207681
23	7.468800951226489E-5	0.0	0.0	0.0024647043139047415	0.0013443841712207681
24	7.468800951226489E-5	0.0	0.0	0.0036597124661009797	0.0013443841712207681
25	7.468800951226489E-5	0.0	0.0	0.004331904551711363	0.0013443841712207681
26	7.468800951226489E-5	0.0	0.0	0.005825664741956661	0.0014937601902452978
27	7.468800951226489E-5	0.0	0.0	0.007916929008300079	0.0016431362092698277
28	7.468800951226489E-5	0.0	0.0	0.014713537873916183	0.0016431362092698277
29	7.468800951226489E-5	0.0	0.0	0.027111747452952157	0.0016431362092698277
30	7.468800951226489E-5	0.0	0.0	0.05153472656346277	0.0016431362092698277
31	7.468800951226489E-5	0.0	0.0	0.10650510156448974	0.0017178242187820924
32	7.468800951226489E-5	0.0	0.0	0.17312680604943	0.0017178242187820924
33	7.468800951226489E-5	0.0	0.0	0.23982319854388257	0.0017178242187820924
34	7.468800951226489E-5	0.0	0.0	0.30988055146638704	0.0017925122282943574
35	7.468800951226489E-5	0.0	0.0	0.3904689137301208	0.0017925122282943574
36	7.468800951226489E-5	0.0	0.0	0.5098950409402324	0.0017925122282943574
37	7.468800951226489E-5	0.0	0.0	0.6931794162833305	0.0017925122282943574
38	7.468800951226489E-5	0.0	0.0	0.9249363097998884	0.0018672002378066223
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGGA	200	0.0	18.701256	2
GTCCTAC	310	0.0	18.453539	1
CGCAATA	365	0.0	18.082052	36
GCATTCG	345	0.0	17.854937	22
ATACGAA	390	0.0	16.922947	40
GGACCGT	105	3.773872E-5	16.762402	6
GTATTAC	120	6.401213E-6	16.501724	1
GTAATAG	80	0.0019871134	16.501724	1
ATAATAC	120	6.4057604E-6	16.50049	3
AAGGCGT	175	3.6252459E-9	16.343344	6
GCATATA	150	1.8358332E-7	16.135017	1
TCTAGCG	410	0.0	16.097439	28
CGAATGC	410	0.0	16.097439	43
TACGAAT	425	0.0	16.046936	41
GTATCAA	2755	0.0	15.812902	1
CCGCTAG	420	0.0	15.714166	36
ATACCGT	295	0.0	15.661482	6
CGCCGGT	450	0.0	15.644324	7
ATATAGT	170	4.489266E-8	15.529874	6
CAATACG	425	0.0	15.529293	38
>>END_MODULE