FastQCFastQC Report
Thu 2 Feb 2017
SRR4062648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036308
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG61860.30378508555680184No Hit
TCCATGTACTCTGCGTTGATACCAC54060.2654804675913467No Hit
GTATCAACGCAGAGTACTTTTTTTT48010.2357698344258334No Hit
TATCAACGCAGAGTACTTTTTTTTT31530.1548390518526667No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28840.14162886950304177No Hit
GAGTACATGGAAGCAGTGGTATCAA28010.13755286528364077No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27630.13568674286993912No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25880.12709275806999729No Hit
GAATAGGACCGCGGTTCTATTTTGT25070.12311497081973846No Hit
GCGCAAGACGGACCAGAGCGAAAGC24920.12237834355117203No Hit
CATGTACTCTGCGTTGATACCACTG24370.11967737689976173No Hit
GTATCTGATCGTCTTCGAACCTCCG22600.11098517513067768No Hit
GTCCTATTCCATTATTCCTAGCTGC22170.10887351029412054No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22000.10803866605641191No Hit
GAATAACGCCGCCGCATCGCCAGTC21860.10735114727241654No Hit
GAACTACGACGGTATCTGATCGTCT21590.10602521818899695No Hit
GATTAAGAGGGACGGCCGGGGGCAT20520.10077061033988964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC400.005277850714.2508126
CGCATCG3750.012.41495613
TTTTCGG2950.012.23888716
CGCGCTA1501.4551915E-1112.03490516
ACGCGCG1501.4551915E-1112.03490513
CGCCAGT3950.012.027184518
GTAAACG1353.7107384E-1011.9648217
AAGACGG4450.011.9536795
ATCGCCA3900.011.93745716
AACCGCG2400.011.8756767
GCGCGCT1701.8189894E-1211.73711715
AGCCCCG3650.011.7138616
TCTAGTA658.0579234E-411.6866532
GAGCGTT907.4588934E-611.6117727
GCGCCGA2950.011.59502119
GCATCGC4050.011.49561214
CGGTCCA4150.011.44727910
TTCGAAG1501.7826096E-1011.3944862
CCGCATC4200.011.31100212
TTCGGAA3300.011.22901518
Produced by FastQC (version 0.11.5)