FastQCFastQC Report
Thu 2 Feb 2017
SRR4062648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036308
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30520.14987909491098594No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26680.13102143683568496No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26470.12999015865969193No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26240.12886066351455672No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA24960.1225747774894564No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24460.12011935326090159No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23110.11348970784380359No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA22510.11054319876953782No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC20540.10086882730903184No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA20450.10042685094789197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT450.001398605624.44425413
TAGGACG1057.7532604E-820.9522174
TTAACGG2600.020.30753335
CGCAATA9100.019.34050836
TAGGCCG708.1263576E-418.8569975
CTAGCGG9300.018.4514729
TAACGGC2950.017.89816736
CGGTCCA10100.017.86124610
ACCGTCG5350.017.6821068
TCTAGCG9750.017.59986328
CGAATGC9650.017.55426843
ATACGAA9650.017.55426840
TAGCGGC9800.017.51006730
AATACGA10050.017.07449339
GTATTAG7000.016.975881
TACGAAT10000.016.93986941
CAATACG10200.016.82339938
GTATAGA2109.094947E-1216.7663021
GATCGAA1053.7766426E-516.76177411
CCGTCGT5650.016.7432339
Produced by FastQC (version 0.11.5)