##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2036308
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30119755950475	32.0	32.0	32.0	32.0	32.0
2	31.437673966806592	32.0	32.0	32.0	32.0	32.0
3	31.51566658874787	32.0	32.0	32.0	32.0	32.0
4	31.612387222365182	32.0	32.0	32.0	32.0	32.0
5	31.549316704545678	32.0	32.0	32.0	32.0	32.0
6	35.15360299129601	36.0	36.0	36.0	36.0	36.0
7	35.16624498847915	36.0	36.0	36.0	36.0	36.0
8	35.11563869512864	36.0	36.0	36.0	36.0	36.0
9	35.242518322375595	36.0	36.0	36.0	36.0	36.0
10	35.120009350255465	36.0	36.0	36.0	36.0	36.0
11	35.24855571946876	36.0	36.0	36.0	36.0	36.0
12	35.15631672615341	36.0	36.0	36.0	36.0	36.0
13	35.192666826432934	36.0	36.0	36.0	36.0	36.0
14	35.15633636954724	36.0	36.0	36.0	36.0	36.0
15	35.12393802902115	36.0	36.0	36.0	36.0	36.0
16	35.13418205890268	36.0	36.0	36.0	36.0	36.0
17	35.104061369891	36.0	36.0	36.0	36.0	36.0
18	35.12170113754894	36.0	36.0	36.0	36.0	36.0
19	35.10183577337024	36.0	36.0	36.0	36.0	36.0
20	35.09751962866128	36.0	36.0	36.0	36.0	36.0
21	35.08115324400828	36.0	36.0	36.0	36.0	36.0
22	35.05831043241003	36.0	36.0	36.0	36.0	36.0
23	35.02122616028616	36.0	36.0	36.0	36.0	36.0
24	34.99581301060547	36.0	36.0	36.0	36.0	36.0
25	34.97337681725947	36.0	36.0	36.0	36.0	36.0
26	34.92332446761492	36.0	36.0	36.0	32.0	36.0
27	34.90924064532477	36.0	36.0	36.0	32.0	36.0
28	34.88934925364925	36.0	36.0	36.0	32.0	36.0
29	34.86116393001452	36.0	36.0	36.0	32.0	36.0
30	34.846763849083736	36.0	36.0	36.0	32.0	36.0
31	34.84873899233318	36.0	36.0	36.0	32.0	36.0
32	34.806456096032626	36.0	36.0	36.0	32.0	36.0
33	34.78185372743219	36.0	36.0	36.0	32.0	36.0
34	34.76285267258195	36.0	36.0	36.0	32.0	36.0
35	34.720564865432934	36.0	36.0	36.0	32.0	36.0
36	34.69985336206507	36.0	36.0	36.0	32.0	36.0
37	34.687750576042525	36.0	36.0	36.0	32.0	36.0
38	34.639398362133825	36.0	36.0	36.0	32.0	36.0
39	34.60986206408854	36.0	36.0	36.0	32.0	36.0
40	34.58951592784589	36.0	36.0	36.0	32.0	36.0
41	34.5568479817395	36.0	36.0	36.0	32.0	36.0
42	34.50084613918916	36.0	36.0	36.0	32.0	36.0
43	34.502584579542976	36.0	36.0	36.0	32.0	36.0
44	34.479435822085854	36.0	36.0	36.0	32.0	36.0
45	34.39636587392477	36.0	36.0	36.0	32.0	36.0
46	34.42816459985425	36.0	36.0	36.0	32.0	36.0
47	34.38210918976893	36.0	36.0	36.0	32.0	36.0
48	34.34959495321926	36.0	36.0	36.0	32.0	36.0
49	34.34539126693997	36.0	36.0	36.0	32.0	36.0
50	33.816306275867895	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	11.0
21	48.0
22	174.0
23	502.0
24	1322.0
25	3115.0
26	6508.0
27	12660.0
28	20969.0
29	31730.0
30	46100.0
31	65732.0
32	97311.0
33	155519.0
34	345760.0
35	1248843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25292042074491	17.783967450029667	11.934039551907457	26.02907257731797
2	16.01105579068646	20.460874032151764	36.79105085997668	26.737019317185094
3	18.77681649939375	24.134788068318265	28.39414563621247	28.694249796075518
4	12.37096087464476	15.525897202820213	35.91978788990429	36.18335403263074
5	14.476248190352345	36.45769696922076	33.27757883385028	15.788476006576607
6	34.60768964826332	35.12190762050311	16.556728491339175	13.713674239894397
7	30.224126322799066	30.42439082122686	20.65130650731938	18.700176348654697
8	28.262227521573358	32.54311233860497	19.725208563733972	19.469451576087703
9	27.303089709415275	14.435930124519473	18.66259917458459	39.59838099148066
10	15.89779149323187	26.58075300985902	31.12569414842941	26.395761348479702
11	37.60663907424613	21.37554829623024	21.75004959956942	19.26776302995421
12	24.49604873133141	23.792422364396742	28.514301372876794	23.197227531395054
13	29.368690787444727	19.264767412395376	25.529487680645563	25.837054119514335
14	23.69474558858483	19.62021462372097	25.003093834527977	31.681945953166217
15	25.45243646835351	26.854189051950883	22.68011518886141	25.013259290834196
16	26.082842084792674	25.798160199734028	23.74635860586905	24.372639109604243
17	24.097582487521535	25.788878696150093	25.38707307538938	24.726465740938995
18	25.162696409384044	24.607033906462085	26.54667172156668	23.683597962587193
19	25.3695413463975	25.456119604696344	25.424739283055413	23.749599765850746
20	25.496044802652644	23.956199160441347	25.71035422932091	24.8374018075851
21	26.69959554252107	24.468204220579597	24.39257715434011	24.43962308255922
22	25.336491336281153	24.52556293055864	25.239993164098944	24.897952569061264
23	24.011544422552973	24.109663174726023	25.582524843982345	26.296267558738656
24	24.238720272178867	25.460637585276885	25.35863926282272	24.942002879721535
25	24.931739206446178	24.294556619136202	25.37302804880205	25.400676125615572
26	24.208223903260215	25.227274066595033	25.95093669523471	24.613565334910042
27	25.418453397030312	24.81363330105269	25.159799008794348	24.60811429312265
28	24.47493208296584	24.366009464187147	25.959727113972935	25.19933133887408
29	24.103573722639208	25.01350483325705	26.02135826210966	24.86156318199408
30	24.556746818261285	25.02867935498952	26.053868078895725	24.360705747853466
31	24.65771386253946	24.913716392608585	24.948092331808354	25.480477413043605
32	24.538232919577982	24.93321246098331	25.024848893192974	25.503705726245734
33	24.274913225307763	24.382018830157325	25.57447105251269	25.768596892022227
34	24.739479489350334	24.939842106400405	25.91120793121669	24.409470473032567
35	25.795311907628903	24.476650879925828	25.556742889582516	24.17129432286275
36	24.43127464018213	25.35962143251414	25.17693786990966	25.03216605739407
37	25.643075605458503	24.97097688561848	25.159799008794348	24.226148500128662
38	24.558858483097843	24.58022067388627	25.50306731594631	25.35785352706958
39	25.11186912785296	24.514366196076427	25.194666032839823	25.17909864323079
40	25.389970475979073	24.88130479279166	25.331629596308613	24.39709513492065
41	23.921184810942155	25.23031879263844	26.09379327685203	24.754703119567374
42	25.8846893495483	25.2261445714499	25.33870121808685	23.55046486091495
43	24.987624661888084	23.83588337324216	25.521090129783904	25.65540183508585
44	24.389335994358415	24.800030250826495	25.57196651979956	25.238667235015527
45	24.656535258909752	25.034621481622622	25.29833404376941	25.010509215698214
46	24.182257390462244	24.74563020212571	25.69352263681262	25.378589770599426
47	24.552056518028234	24.599200709520083	26.074421034952504	24.77432173749918
48	25.340272689593125	25.58002031126922	24.694447008998637	24.385259990139016
49	24.16020767006154	25.249188850207755	25.601198640480042	24.98940483925066
50	24.181668088357995	25.892608531031915	24.74440248940852	25.18132089120157
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	33.0
2	40.0
3	170.0
4	300.0
5	343.5
6	387.0
7	363.0
8	339.0
9	457.0
10	575.0
11	889.5
12	1204.0
13	2116.5
14	3029.0
15	4057.5
16	5086.0
17	5805.5
18	6525.0
19	6970.5
20	7416.0
21	8492.5
22	9569.0
23	10353.0
24	11137.0
25	12583.0
26	14029.0
27	17612.5
28	21196.0
29	24106.0
30	27016.0
31	29490.0
32	31964.0
33	36412.5
34	40861.0
35	46657.5
36	52454.0
37	62845.0
38	73236.0
39	80584.0
40	87932.0
41	96330.5
42	104729.0
43	108448.0
44	112167.0
45	126390.0
46	140613.0
47	151064.5
48	161516.0
49	163031.5
50	164547.0
51	154347.5
52	144148.0
53	139909.0
54	135670.0
55	137422.0
56	139174.0
57	134971.0
58	130768.0
59	119696.5
60	108625.0
61	95667.5
62	82710.0
63	72487.0
64	62264.0
65	53312.5
66	44361.0
67	39296.0
68	34231.0
69	31825.5
70	29420.0
71	22784.5
72	16149.0
73	14440.0
74	12731.0
75	9058.5
76	5386.0
77	4988.0
78	4590.0
79	3989.5
80	3389.0
81	2615.0
82	1841.0
83	1646.5
84	1452.0
85	1198.5
86	945.0
87	666.5
88	388.0
89	244.0
90	100.0
91	68.5
92	37.0
93	24.5
94	12.0
95	12.5
96	13.0
97	10.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014732545371328896
2	0.004419763611398669
3	7.366272685664447E-4
4	3.437593919976742E-4
5	0.0
6	5.893018148531558E-4
7	4.9108484571096314E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	4.9108484571096314E-5
47	9.821696914219263E-5
48	0.0
49	4.9108484571096314E-5
50	4.9108484571096314E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2036308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.11987747371987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24140891550756	39.21449828216452
2	12.978300156753738	13.009416273473134
3	3.826584612763252	5.7536385580354805
4	1.5354009840805964	3.0781643678059365
5	0.7945815309049253	1.991216448591781
6	0.49549099665948243	1.490036882514276
7	0.3325984188146188	1.1668854399259163
8	0.2437533391713566	0.9773509994462781
9	0.18500589007634669	0.8345225288288685
>10	1.1593320138602643	10.856591153852508
>50	0.10096006203752443	3.5480267944300157
>100	0.08836127757226274	9.384637523087424
>500	0.012607516919704848	4.340818255481175
>1k	0.005614284878306065	4.354196492362769
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3052	0.14987909491098594	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2668	0.13102143683568496	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2647	0.12999015865969193	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2624	0.12886066351455672	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2496	0.1225747774894564	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2446	0.12011935326090159	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2311	0.11348970784380359	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2251	0.11054319876953782	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2054	0.10086882730903184	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2045	0.10042685094789197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.910848457109632E-5
7	0.0	0.0	0.0	0.0	4.910848457109632E-5
8	0.0	0.0	0.0	0.0	4.910848457109632E-5
9	0.0	0.0	0.0	0.0	4.910848457109632E-5
10	0.0	0.0	0.0	0.0	4.910848457109632E-5
11	0.0	0.0	0.0	0.0	4.910848457109632E-5
12	0.0	0.0	0.0	0.0	4.910848457109632E-5
13	0.0	0.0	0.0	0.0	4.910848457109632E-5
14	0.0	0.0	0.0	0.0	4.910848457109632E-5
15	0.0	0.0	0.0	0.0	1.9643393828438528E-4
16	0.0	0.0	0.0	1.4732545371328896E-4	2.455424228554816E-4
17	0.0	0.0	0.0	1.4732545371328896E-4	2.455424228554816E-4
18	0.0	0.0	0.0	2.455424228554816E-4	2.946509074265779E-4
19	0.0	0.0	0.0	2.455424228554816E-4	2.946509074265779E-4
20	0.0	0.0	0.0	4.910848457109632E-4	2.946509074265779E-4
21	0.0	0.0	0.0	6.384102994242522E-4	2.946509074265779E-4
22	0.0	0.0	0.0	0.0014241460525617933	2.946509074265779E-4
23	0.0	0.0	0.0	0.002750075135981394	2.946509074265779E-4
24	0.0	0.0	0.0	0.004959956941680728	2.946509074265779E-4
25	0.0	0.0	0.0	0.0067278623862401955	2.946509074265779E-4
26	0.0	0.0	0.0	0.009281503583937204	2.946509074265779E-4
27	0.0	0.0	0.0	0.01212979568906079	2.946509074265779E-4
28	0.0	0.0	0.0	0.01920141746729866	2.946509074265779E-4
29	0.0	0.0	0.0	0.030692802856935198	2.946509074265779E-4
30	0.0	0.0	0.0	0.0506308475928003	2.946509074265779E-4
31	0.0	0.0	0.0	0.09625262975934878	2.946509074265779E-4
32	0.0	0.0	0.0	0.15164700035554543	2.946509074265779E-4
33	0.0	0.0	0.0	0.20458594672318725	2.946509074265779E-4
34	0.0	0.0	0.0	0.2667081797056241	3.4375939199767424E-4
35	0.0	0.0	0.0	0.3331028508457463	3.4375939199767424E-4
36	0.0	0.0	0.0	0.43141803695708114	3.4375939199767424E-4
37	0.0	0.0	0.0	0.5779577549172326	3.4375939199767424E-4
38	0.0	0.0	0.0	0.7599537987377155	3.4375939199767424E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATT	45	0.0013986056	24.444254	13
TAGGACG	105	7.7532604E-8	20.952217	4
TTAACGG	260	0.0	20.307533	35
CGCAATA	910	0.0	19.340508	36
TAGGCCG	70	8.1263576E-4	18.856997	5
CTAGCGG	930	0.0	18.45147	29
TAACGGC	295	0.0	17.898167	36
CGGTCCA	1010	0.0	17.861246	10
ACCGTCG	535	0.0	17.682106	8
TCTAGCG	975	0.0	17.599863	28
CGAATGC	965	0.0	17.554268	43
ATACGAA	965	0.0	17.554268	40
TAGCGGC	980	0.0	17.510067	30
AATACGA	1005	0.0	17.074493	39
GTATTAG	700	0.0	16.97588	1
TACGAAT	1000	0.0	16.939869	41
CAATACG	1020	0.0	16.823399	38
GTATAGA	210	9.094947E-12	16.766302	1
GATCGAA	105	3.7766426E-5	16.761774	11
CCGTCGT	565	0.0	16.743233	9
>>END_MODULE