FastQCFastQC Report
Thu 2 Feb 2017
SRR4062647_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062647_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338966
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCAC9530.28114914180183265No Hit
GTACATGGAAGCAGTGGTATCAACG9320.27495383017765795No Hit
GTATCAACGCAGAGTACTTTTTTTT8440.24899252432397348No Hit
GTCCTAAAGTGTGTATTTCTCATTT8250.24338724237829165No Hit
GTCCTACAGTGGACATTTCTAAATT7440.2194910403993321No Hit
CTGTAGGACGTGGAATATGGCAAGA6770.19972504616982234No Hit
TTGTAGAACAGTGTATATCAATGAG5950.17553382935161638No Hit
CTTTAGGACGTGAAATATGGCGAGG5910.17435376999463073No Hit
GATATACACTGTTCTACAAATCCCG5810.1714036216021666No Hit
TATCAACGCAGAGTACTTTTTTTTT5800.1711086067629202No Hit
GTCCTACAGTGTGCATTTCTCATTT5690.16786344353120963No Hit
GTGTATATCAATGAGTTACAATGAA4690.13836195960656822No Hit
CTGTAGGACCTGGAATATGGCGAGA4300.12685638087595807No Hit
GAGTACATGGAAGCAGTGGTATCAA4190.12361121764424751No Hit
CATGTACTCTGCGTTGATACCACTG4000.11800593569856563No Hit
GTGTATATCAATGAGTTACAATGAG3780.11151560923514453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTG250.006020925519.00088910
CCGGTCT456.740632E-414.7784679
TCAAAGC456.748188E-414.7762853
CAATGAA1101.0913936E-1114.68250319
AGTTGTC400.005266192414.2506668
AGGCACC400.005266192414.2506666
TATACAG400.005266192414.2506665
GACTAAA400.005266192414.25066614
GTTACAA1800.014.25066515
ACTAACC400.00527135714.2485613
GTTATAT400.005276526314.2464561
TAGGACC2150.014.1401954
ATAGGAC551.9547103E-413.8167853
TATAGAG500.001494799713.3006225
TAACTTT500.001494799713.3006226
CCACCTT655.4255812E-513.15445913
CCTTTAA655.4406475E-513.1505741
TTACAAT1900.013.00060616
TTGTAGA1352.7284841E-1112.6635161
GTCCTAA2800.012.5504481
Produced by FastQC (version 0.11.5)