##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062647_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338966
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.54981620575515	32.0	32.0	32.0	14.0	32.0
2	27.766112235445444	32.0	21.0	32.0	14.0	32.0
3	28.202456883581245	32.0	21.0	32.0	14.0	32.0
4	27.657644129499715	32.0	21.0	32.0	14.0	32.0
5	27.651850038056914	32.0	21.0	32.0	14.0	32.0
6	30.800572918817817	36.0	21.0	36.0	14.0	36.0
7	30.812618374704247	36.0	27.0	36.0	14.0	36.0
8	30.21243428544456	36.0	21.0	36.0	14.0	36.0
9	31.291905382840756	36.0	32.0	36.0	14.0	36.0
10	29.600248992524325	36.0	21.0	36.0	14.0	36.0
11	31.54502811491418	36.0	32.0	36.0	14.0	36.0
12	30.473419162983898	36.0	27.0	36.0	14.0	36.0
13	30.99909135429512	36.0	32.0	36.0	14.0	36.0
14	30.297669972799632	36.0	27.0	36.0	14.0	36.0
15	30.114698819350615	36.0	21.0	36.0	14.0	36.0
16	30.459417758713265	36.0	27.0	36.0	14.0	36.0
17	30.084433246992322	36.0	21.0	36.0	14.0	36.0
18	30.25069771009482	36.0	27.0	36.0	14.0	36.0
19	30.297436911076627	36.0	27.0	36.0	14.0	36.0
20	29.997433370898555	36.0	21.0	36.0	14.0	36.0
21	30.014806794781776	36.0	21.0	36.0	14.0	36.0
22	29.847093808818585	36.0	21.0	36.0	14.0	36.0
23	29.797687673689985	36.0	21.0	36.0	14.0	36.0
24	29.79446906179381	36.0	21.0	36.0	14.0	36.0
25	28.88877645545571	32.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	21.0
5	33.0
6	67.0
7	23.0
8	52.0
9	57.0
10	27.0
11	28.0
12	18.0
13	17.0
14	603.0
15	912.0
16	1167.0
17	1630.0
18	2200.0
19	3170.0
20	4271.0
21	5529.0
22	7148.0
23	9239.0
24	11373.0
25	14067.0
26	16837.0
27	19755.0
28	22771.0
29	25574.0
30	28441.0
31	31861.0
32	35285.0
33	36249.0
34	40070.0
35	20468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.618929604059716	18.600342243464922	11.992092995810468	25.788635156664895
2	14.8355538771994	21.310435572758706	40.62710204274301	23.226908507298884
3	19.734124783157696	25.37910525259916	29.116463493786803	25.770306470456344
4	11.755350851828466	16.688402504440603	37.95785411220413	33.5983925315268
5	12.602110680895267	38.47654405515157	34.97644961752762	13.944895646425536
6	30.863632741364704	37.549621767759795	18.340795660142913	13.245949830732586
7	27.560747360061878	31.80118025972799	21.284528796507047	19.353543583703086
8	25.635203486569562	36.19973366482515	20.393538271655974	17.77152457694931
9	27.511302596570392	14.31494895744436	19.16199847034748	39.01174997563777
10	15.056739306368625	28.224629761996184	33.52049526532493	23.198135666310264
11	35.91443837631401	21.784424169209277	23.86704985482761	18.434087599649104
12	23.624187244652283	24.584870712447824	31.537123089784906	20.253818953114983
13	29.976901764563408	21.304430017072608	25.618365164789076	23.100303053574912
14	21.537107274940034	21.738860728084788	26.408729455413372	30.3153025415618
15	23.855050889908853	30.741580865630556	23.33433754511569	22.0690306993449
16	23.020943166077444	27.030890505429984	27.665594551409846	22.282571777082723
17	21.62337796603661	27.372755441733794	27.890861077387967	23.11300551484163
18	21.804002658592424	26.66893139354553	30.543091352189645	20.983974595672404
19	24.715742083760446	25.413499629260915	26.468407417144984	23.402350869833654
20	24.379862592082798	26.669600713623232	27.571081718130635	21.37945497616333
21	24.749799130352585	25.233363266849416	25.13795018432744	24.878887418470555
22	23.644572083650957	27.92344011298433	26.294267463229865	22.137720340134848
23	22.784570798904582	27.4465954310327	26.72252076064768	23.04631300941504
24	23.317390777738716	26.869754539787948	27.2145036882546	22.59835099421873
25	23.310567299625422	26.719859622588	27.382457550012408	22.587115527774166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	37.0
2	131.0
3	225.0
4	225.0
5	225.0
6	507.5
7	790.0
8	790.0
9	790.0
10	932.0
11	1074.0
12	1074.0
13	1074.0
14	1236.0
15	1398.0
16	1398.0
17	1398.0
18	2355.5
19	3313.0
20	3313.0
21	3313.0
22	5492.5
23	7672.0
24	7672.0
25	7672.0
26	12208.5
27	16745.0
28	16745.0
29	16745.0
30	22014.0
31	27283.0
32	27283.0
33	27283.0
34	29266.5
35	31250.0
36	31250.0
37	31250.0
38	32394.5
39	33539.0
40	33539.0
41	33539.0
42	35525.0
43	37511.0
44	37511.0
45	37511.0
46	41203.0
47	44895.0
48	44895.0
49	44895.0
50	41557.0
51	38219.0
52	38219.0
53	38219.0
54	33449.5
55	28680.0
56	28680.0
57	28680.0
58	25429.0
59	22178.0
60	22178.0
61	22178.0
62	19455.0
63	16732.0
64	16732.0
65	16732.0
66	14456.0
67	12180.0
68	12180.0
69	12180.0
70	9647.5
71	7115.0
72	7115.0
73	7115.0
74	5684.0
75	4253.0
76	4253.0
77	4253.0
78	3175.0
79	2097.0
80	2097.0
81	2097.0
82	1578.0
83	1059.0
84	1059.0
85	1059.0
86	759.0
87	459.0
88	459.0
89	459.0
90	284.0
91	109.0
92	109.0
93	109.0
94	60.5
95	12.0
96	12.0
97	12.0
98	76.5
99	141.0
100	141.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007670385820406767
2	0.0029501483924641407
3	0.0029501483924641407
4	0.014160712283827877
5	0.034811751031076865
6	0.045137270404701356
7	0.066968368508936
8	0.08702937757769216
9	0.0964698524335774
10	0.11800593569856563
11	0.11889098021630488
12	0.13482178153561122
13	0.1215461137695226
14	0.12803644023294372
15	0.11564581698459432
16	0.11299068343137661
17	0.125381306679726
18	0.1301015441076686
19	0.1336417221786256
20	0.1215461137695226
21	0.12921649958992937
22	0.15104759769416404
23	0.13718190024958257
24	0.13541181121410406
25	0.13275667766088634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	338966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.200764220988276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7090165728259	48.96436989077678
2	10.824874463719473	12.816816816969068
3	2.6551081904389204	4.715533018901681
4	1.0490492382749401	2.484180664452883
5	0.5696014336352646	1.68604200862891
6	0.35216943427112907	1.2509219786474366
7	0.25215791145408517	1.0449558743715095
8	0.18290907459630507	0.866268559924401
9	0.16862613765274573	0.8984516595008503
>10	1.063782460608035	12.533369108689369
>50	0.11347769111034499	4.757086037168269
>100	0.05375259052595243	5.656939908801053
>500	0.005474800886902562	2.325064473167827
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCAC	953	0.28114914180183265	No Hit
GTACATGGAAGCAGTGGTATCAACG	932	0.27495383017765795	No Hit
GTATCAACGCAGAGTACTTTTTTTT	844	0.24899252432397348	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	825	0.24338724237829165	No Hit
GTCCTACAGTGGACATTTCTAAATT	744	0.2194910403993321	No Hit
CTGTAGGACGTGGAATATGGCAAGA	677	0.19972504616982234	No Hit
TTGTAGAACAGTGTATATCAATGAG	595	0.17553382935161638	No Hit
CTTTAGGACGTGAAATATGGCGAGG	591	0.17435376999463073	No Hit
GATATACACTGTTCTACAAATCCCG	581	0.1714036216021666	No Hit
TATCAACGCAGAGTACTTTTTTTTT	580	0.1711086067629202	No Hit
GTCCTACAGTGTGCATTTCTCATTT	569	0.16786344353120963	No Hit
GTGTATATCAATGAGTTACAATGAA	469	0.13836195960656822	No Hit
CTGTAGGACCTGGAATATGGCGAGA	430	0.12685638087595807	No Hit
GAGTACATGGAAGCAGTGGTATCAA	419	0.12361121764424751	No Hit
CATGTACTCTGCGTTGATACCACTG	400	0.11800593569856563	No Hit
GTGTATATCAATGAGTTACAATGAG	378	0.11151560923514453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.950148392464141E-4	0.0
11	0.0	0.0	0.0	2.950148392464141E-4	0.0
12	0.0	0.0	0.0	2.950148392464141E-4	0.0
13	0.0	0.0	0.0	2.950148392464141E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTG	25	0.0060209255	19.000889	10
CCGGTCT	45	6.740632E-4	14.778467	9
TCAAAGC	45	6.748188E-4	14.776285	3
CAATGAA	110	1.0913936E-11	14.682503	19
AGTTGTC	40	0.0052661924	14.250666	8
AGGCACC	40	0.0052661924	14.250666	6
TATACAG	40	0.0052661924	14.250666	5
GACTAAA	40	0.0052661924	14.250666	14
GTTACAA	180	0.0	14.250665	15
ACTAACC	40	0.005271357	14.248561	3
GTTATAT	40	0.0052765263	14.246456	1
TAGGACC	215	0.0	14.140195	4
ATAGGAC	55	1.9547103E-4	13.816785	3
TATAGAG	50	0.0014947997	13.300622	5
TAACTTT	50	0.0014947997	13.300622	6
CCACCTT	65	5.4255812E-5	13.154459	13
CCTTTAA	65	5.4406475E-5	13.150574	1
TTACAAT	190	0.0	13.000606	16
TTGTAGA	135	2.7284841E-11	12.663516	1
GTCCTAA	280	0.0	12.550448	1
>>END_MODULE