FastQCFastQC Report
Thu 2 Feb 2017
SRR4062647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338966
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5190.15311270156888893No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC4060.11977602473404413No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA3570.10532029761096984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAG301.2991855E-436.66654215
ATTACAC300.00573935229.3332333
TCTAGCG300.00573935229.33323328
ACGTGAT300.00573935229.33323337
CCGACAA300.00573935229.33323314
AGTACGC554.9473656E-627.9999073
CACTCCG407.021762E-427.49990834
CGGTCCA407.021762E-427.49990810
CTAGCAC407.021762E-427.4999083
TGCGTAG655.910206E-727.07683413
TACGCCT508.3242485E-526.3999125
TAGATGC609.726238E-625.66657814
ATATATC801.3040699E-724.7499183
ATAATCT551.5903577E-423.9999227
GTCCTAG651.806384E-523.6957241
CTGTGCG651.8084484E-523.6922310
ATAATAT500.00257669521.9999263
AATATAT1004.5994966E-821.9999262
TACTAGA602.8669412E-421.9999262
TATACTT500.00257669521.9999265
Produced by FastQC (version 0.11.5)