##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338966
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74738469345008	32.0	32.0	32.0	32.0	32.0
2	30.41868210971012	32.0	32.0	32.0	21.0	32.0
3	30.99743927119534	32.0	32.0	32.0	32.0	32.0
4	31.321861189617838	32.0	32.0	32.0	32.0	32.0
5	31.076261335945198	32.0	32.0	32.0	32.0	32.0
6	34.697096463952136	36.0	36.0	36.0	32.0	36.0
7	34.71159939344949	36.0	36.0	36.0	32.0	36.0
8	34.61162476472567	36.0	36.0	36.0	32.0	36.0
9	34.87740953369954	36.0	36.0	36.0	32.0	36.0
10	34.58147719830308	36.0	36.0	36.0	32.0	36.0
11	34.900223031218474	36.0	36.0	36.0	32.0	36.0
12	34.7413073877616	36.0	36.0	36.0	32.0	36.0
13	34.83751172683986	36.0	36.0	36.0	32.0	36.0
14	34.76464896184278	36.0	36.0	36.0	32.0	36.0
15	34.66431441501508	36.0	36.0	36.0	32.0	36.0
16	34.73050689449679	36.0	36.0	36.0	32.0	36.0
17	34.69488680280618	36.0	36.0	36.0	32.0	36.0
18	34.72151484219656	36.0	36.0	36.0	32.0	36.0
19	34.715163172707584	36.0	36.0	36.0	32.0	36.0
20	34.68803950838727	36.0	36.0	36.0	32.0	36.0
21	34.668757338494125	36.0	36.0	36.0	32.0	36.0
22	34.637055043868706	36.0	36.0	36.0	32.0	36.0
23	34.58361605588761	36.0	36.0	36.0	32.0	36.0
24	34.576603553158726	36.0	36.0	36.0	32.0	36.0
25	34.563050571443746	36.0	36.0	36.0	32.0	36.0
26	34.46105214092269	36.0	36.0	36.0	32.0	36.0
27	34.43859266121086	36.0	36.0	36.0	32.0	36.0
28	34.41893287232348	36.0	36.0	36.0	32.0	36.0
29	34.374571490945996	36.0	36.0	36.0	32.0	36.0
30	34.36236377689798	36.0	36.0	36.0	32.0	36.0
31	34.33656177905748	36.0	36.0	36.0	32.0	36.0
32	34.279331850392076	36.0	36.0	36.0	32.0	36.0
33	34.27824324563525	36.0	36.0	36.0	32.0	36.0
34	34.24075866016061	36.0	36.0	36.0	32.0	36.0
35	34.21402736557649	36.0	36.0	36.0	32.0	36.0
36	34.14512664987049	36.0	36.0	36.0	32.0	36.0
37	34.13196013759492	36.0	36.0	36.0	32.0	36.0
38	34.05571945268847	36.0	36.0	36.0	32.0	36.0
39	33.97594449000785	36.0	36.0	36.0	32.0	36.0
40	33.96672822642979	36.0	36.0	36.0	32.0	36.0
41	33.9466406660255	36.0	36.0	36.0	32.0	36.0
42	33.89072650354313	36.0	36.0	36.0	32.0	36.0
43	33.85947558162176	36.0	36.0	36.0	32.0	36.0
44	33.81427045780403	36.0	36.0	36.0	32.0	36.0
45	33.72275980481818	36.0	36.0	36.0	32.0	36.0
46	33.71765604809922	36.0	36.0	36.0	32.0	36.0
47	33.6944885327732	36.0	36.0	36.0	27.0	36.0
48	33.63047621295351	36.0	36.0	36.0	27.0	36.0
49	33.59763221090021	36.0	36.0	36.0	21.0	36.0
50	32.929069582199986	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	4.0
22	27.0
23	99.0
24	253.0
25	650.0
26	1332.0
27	2519.0
28	4512.0
29	7379.0
30	11433.0
31	17720.0
32	27278.0
33	45348.0
34	85783.0
35	134627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95060999896735	19.06412734005045	12.152182571879564	24.83308008910263
2	15.138882742546947	21.6987505716098	40.2563762151318	22.90599047071145
3	20.27223969365659	25.242354690440926	29.784108140639475	24.701297475263008
4	12.73372550639297	17.33035171669135	38.317412365842	31.618510411073675
5	13.886053468489465	37.87282500309765	34.095750016225814	14.145371512187063
6	31.60739309368224	37.790332335196844	17.6029973596094	12.999277211511512
7	27.878017264268394	31.89139913737661	21.186785695320477	19.043797903034523
8	26.09966781329101	36.108046234725606	20.167214410884867	17.625071541098517
9	27.744375542089767	14.29700913955972	18.919006626033287	39.03960869231722
10	15.088238938418602	28.423794716874255	33.40335018851448	23.084616156192656
11	36.64113804924388	21.245198633491263	23.62803349008455	18.485629827180308
12	23.781441206492687	24.51366803750229	31.33057592796918	20.37431482803585
13	30.447891528943906	20.914486998696034	25.505507927048733	23.132113545311327
14	21.88715092369146	21.65526926004378	26.139789831428523	30.317789984836235
15	24.346984653328064	30.385053368184415	23.0194178767192	22.24854410176832
16	23.498521975655375	26.86257618758224	27.250225686352024	22.388676150410365
17	22.083335791790326	27.24786556763805	27.5977531669843	23.071045473587322
18	22.344423924523404	26.643085147182905	30.156121852929203	20.85636907536449
19	25.30725795507514	25.47394133924936	26.077246685508282	23.141554020167217
20	24.567360738245135	26.685862298873637	27.375902007870994	21.370874955010237
21	25.223768755568404	25.247664957547368	25.00309765581209	24.52546863107214
22	24.07881616445307	27.78125239699557	25.96307594271992	22.17685549583144
23	23.415327790987885	27.27707203672345	26.29732775558611	23.01027241670256
24	23.733058772856275	26.97409179681738	26.652820636878033	22.640028793448312
25	24.12749361292873	26.597062832260466	26.78026704743249	22.49517650737832
26	21.197406229533343	27.592147885038614	28.302838632783228	22.90760725264481
27	23.26457520813297	27.17499690234419	27.31453892130774	22.2458889682151
28	22.178330570027672	27.71221892461191	26.708873456334853	23.400577049025568
29	22.861879952561615	26.205873155419717	26.36105096086333	24.571195931155337
30	22.913802564268984	27.058761055681103	26.729524495082103	23.297911884967814
31	23.699722096021432	26.14096989078551	26.663441171090906	23.49586684210216
32	22.557719653298562	27.873887056518942	26.94842550580294	22.619967784379554
33	22.773670515626936	27.134284854528186	27.81399904415192	22.27804558569296
34	22.46567502345368	27.622829428320244	28.100753467899438	21.81074208032664
35	23.078125829729235	27.546125570116175	27.078822064749858	22.296926535404733
36	21.98303074644655	27.375902007870994	27.59922824118053	23.041839004501927
37	23.1014320020297	27.059351085359594	25.97723665500375	23.86198025760696
38	22.998176808293458	27.907223733353785	26.04361499383419	23.050984464518564
39	23.67435081984624	25.9769416401645	26.660786037537687	23.687921502451573
40	24.790981986393916	26.548385383784805	26.628924434899076	22.031708194922203
41	23.458104942678617	25.460370656644027	28.470702076314442	22.610822324362914
42	23.600597110034634	27.883622546214077	26.921874170270765	21.593906173480526
43	22.214617395254983	27.19387785205596	27.656166105155087	22.93533864753397
44	22.05501436722267	27.076461946035884	27.471486815786832	23.39703687095461
45	22.42053775304898	27.203908356590333	27.209218623696774	23.16633526666391
46	22.851849448027238	25.812028345025755	26.841040104317248	24.495082102629762
47	23.731288683820793	26.034764548656796	26.958160995498076	23.27578577202433
48	22.12021264669613	26.86818146952792	26.38730728155626	24.624298602219692
49	22.91055740103727	25.951865378828554	27.704548538791503	23.43302868134267
50	22.025217868458782	28.079512399473693	26.063676002902948	23.831593729164577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	5.0
2	10.0
3	21.0
4	32.0
5	52.0
6	72.0
7	70.0
8	68.0
9	84.5
10	101.0
11	138.5
12	176.0
13	282.0
14	388.0
15	516.0
16	644.0
17	851.5
18	1059.0
19	1245.0
20	1431.0
21	1779.0
22	2127.0
23	2334.0
24	2541.0
25	3300.5
26	4060.0
27	5750.0
28	7440.0
29	8766.0
30	10092.0
31	10832.0
32	11572.0
33	13914.0
34	16256.0
35	18793.5
36	21331.0
37	22274.0
38	23217.0
39	22020.0
40	20823.0
41	20418.0
42	20013.0
43	19087.0
44	18161.0
45	18698.0
46	19235.0
47	19765.5
48	20296.0
49	19853.5
50	19411.0
51	18567.0
52	17723.0
53	16582.5
54	15442.0
55	15179.5
56	14917.0
57	14540.5
58	14164.0
59	12952.5
60	11741.0
61	10406.5
62	9072.0
63	8594.5
64	8117.0
65	7214.0
66	6311.0
67	6144.5
68	5978.0
69	5464.0
70	4950.0
71	4130.0
72	3310.0
73	2850.0
74	2390.0
75	2031.0
76	1672.0
77	1383.0
78	1094.0
79	894.5
80	695.0
81	537.5
82	380.0
83	302.5
84	225.0
85	172.5
86	120.0
87	88.5
88	57.0
89	42.5
90	28.0
91	20.5
92	13.0
93	8.0
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009145460016638837
2	0.003245163231710555
3	0.0
4	0.0
5	0.0
6	2.9501483924641414E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	338966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.68070810960383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.08623775997731	58.437830597373534
2	9.770050461642825	13.42027967944382
3	2.3083815910904586	4.756238467897999
4	0.8529513035543421	2.343251980444874
5	0.44319111497043767	1.521933980202725
6	0.2686376712921568	1.1070135293556194
7	0.18436597527299578	0.8863670013146943
8	0.143236857975579	0.7870087066526015
9	0.09630764527280496	0.5953029546334305
>10	0.7550279550787414	10.007311734937243
>50	0.06580556137314852	3.124412999309987
>100	0.025376000790910156	2.8597373779220345
>500	4.301017083205111E-4	0.15331099051146949
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	519	0.15311270156888893	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	406	0.11977602473404413	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	357	0.10532029761096984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.950148392464141E-4	0.0
16	0.0	0.0	0.0	2.950148392464141E-4	0.0
17	0.0	0.0	0.0	2.950148392464141E-4	0.0
18	0.0	0.0	0.0	2.950148392464141E-4	0.0
19	0.0	0.0	0.0	2.950148392464141E-4	0.0
20	0.0	0.0	0.0	5.900296784928282E-4	0.0
21	0.0	0.0	0.0	8.850445177392423E-4	0.0
22	0.0	0.0	0.0	0.0014750741962320706	0.0
23	0.0	0.0	0.0	0.002065103874724899	0.0
24	0.0	0.0	0.0	0.003245163231710555	0.0
25	0.0	0.0	0.0	0.004720237427942625	0.0
26	0.0	0.0	0.0	0.005605281945681868	0.0
27	0.0	0.0	0.0	0.007080356141913938	0.0
28	0.0	0.0	0.0	0.011800593569856565	0.0
29	0.0	0.0	0.0	0.019175964551016916	0.0
30	0.0	0.0	0.0	0.03451673619183045	0.0
31	0.0	0.0	0.0	0.07463875432934276	0.0
32	0.0	0.0	0.0	0.1259713363582188	0.0
33	0.0	0.0	0.0	0.16638836933497755	0.0
34	0.0	0.0	0.0	0.230111574612203	0.0
35	0.0	0.0	0.0	0.2888195276222394	0.0
36	0.0	0.0	0.0	0.3938448103939628	0.0
37	0.0	0.0	0.0	0.5448924080881268	0.0
38	0.0	0.0	0.0	0.7481576323289062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAG	30	1.2991855E-4	36.666542	15
ATTACAC	30	0.005739352	29.333233	3
TCTAGCG	30	0.005739352	29.333233	28
ACGTGAT	30	0.005739352	29.333233	37
CCGACAA	30	0.005739352	29.333233	14
AGTACGC	55	4.9473656E-6	27.999907	3
CACTCCG	40	7.021762E-4	27.499908	34
CGGTCCA	40	7.021762E-4	27.499908	10
CTAGCAC	40	7.021762E-4	27.499908	3
TGCGTAG	65	5.910206E-7	27.076834	13
TACGCCT	50	8.3242485E-5	26.399912	5
TAGATGC	60	9.726238E-6	25.666578	14
ATATATC	80	1.3040699E-7	24.749918	3
ATAATCT	55	1.5903577E-4	23.99992	27
GTCCTAG	65	1.806384E-5	23.695724	1
CTGTGCG	65	1.8084484E-5	23.69223	10
ATAATAT	50	0.002576695	21.999926	3
AATATAT	100	4.5994966E-8	21.999926	2
TACTAGA	60	2.8669412E-4	21.999926	2
TATACTT	50	0.002576695	21.999926	5
>>END_MODULE