FastQCFastQC Report
Thu 2 Feb 2017
SRR4062646_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062646_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464397
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32800.22398297729372568No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20530.14019422328781062No Hit
TATCAACGCAGAGTACTTTTTTTTT20430.13951134835703705No Hit
GTACATGGAAGCAGTGGTATCAACG19020.12988281183312994No Hit
TCCATGTACTCTGCGTTGATACCAC16550.11301580104302318No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15590.10646020170759707No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15170.10359212699834813No Hit
GCGCAAGACGGACCAGAGCGAAAGC14820.1012020647406407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA1001.00462785E-813.30217910
TCGCGTA1001.00462785E-813.3021799
ACACCGT905.395741E-712.6683086
GCGTAAC1051.9912477E-812.66744111
CGAACGA853.9341394E-612.29655316
CGTTATT1551.8189894E-1212.2500152
GGTAAAT701.09892644E-412.2045961
ACGCGCG1251.4097168E-912.16032913
CGGCTAA550.003064533712.092899
GTGTTAA1103.8549842E-812.0813171
CCTATTC3100.011.9458073
GCTCGTA1459.640644E-1111.7950369
CAAGACG3150.011.75980954
CGCATCG2350.011.723721513
GCGCGCT1302.6066118E-911.69422315
CCGCACA658.034927E-411.6902244
CGCCACT1552.5465852E-1111.64826517
GCGTTAT1800.011.60191
AATCCCG1406.7666406E-1011.53878819
ATCCCCG1406.7848305E-1011.5364212
Produced by FastQC (version 0.11.5)